Molecular characterization of TP53 gene in human populations exposed to low-dose Ionizing radiation

• Autor(a) principal: Costa, Igor Brasil
• Data de Publicação: 2013
• Outros Autores: Alencar, Dayse Oliveira de, Moraes, Milene Raiol, Pessoa, Igor A, Brito, Alexandre W. M, Jati, Schneyder R, Santos, Sidney E. B, Burbano, Rommel M. R, Santos, Andrea Kely Campos Ribeiro dos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/979
• topic: Genes p53; Radiação Ionizante; Mutação / efeitos de radiação; Genômica; Genes Neoplásicos / efeitos de radiação

Ionizing radiation, such as that emitted by uranium, may cause mutations and consequently lead to neoplasia in human cells. The TP53 gene acts to maintain genomic integrity and constitutes an important biomarker of susceptibility. The present study investigated themain alterations observed in exons 4, 5, 6, 7, and 8 of the TP53 gene and adjacent introns in Amazonian populations exposed to radioactivity. Samples were collected from 163 individuals. Occurrence of the following alterations was observed: (i) a missense exchange in exon 4 (Arg72Pro); (ii) 2 synonymous exchanges, 1 in exon 5 (His179His), and another in exon 6 (Arg213Arg); (iii) 4 intronic exchanges, 3 in intron 7 (C - T at position 13.436; C - T at position 13.491; T - G at position 13.511) and 1in intron 8 (T - G at position 13.958). Alteration of codon 72 was found to be an important risk factor for cancer development (P = 0.024; OR = 6.48; CI: 1.29¨C32.64) when adjusted for age and smoking.Thus, TP53 gene may be an important biomarker for carcinogenesis susceptibility in human populations exposed to ionizing radiation.