Phylogenomic perspective on a unique mycobacterium bovis clade dominating bovine tuberculosis infections among cattle and buffalos in Northern Brazil

• Autor(a) principal: Conceição, Marília Lima da
• Data de Publicação: 2020
• Outros Autores: Conceição, Emilyn Costa, Furlaneto, Ismari Perini, Silva, Sandro Patroca da, Guimarães, Arthur Emil dos Santos, Gomes, Pedro, Boschiroli, María Laura, Michelet, Lorraine, Kohl, Thomas Andreas, Kranzer, Katharina, Francez, Loreno da Costa, Lima, Luana Nepomuceno Gondim Costa, Portugal, Isabel, Perdigão, João, Lima, Karla valéria Batista
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/4072
• topic: Tuberculose Bovina / diagnóstico; Tuberculose Bovina / transmissão; Mycobacterium bovis / isolamento & purificação; Mycobacterium bovis / virologia; Sequenciamento Completo do Genoma / métodos; Genoma Bacteriano / genético; Gado; Ilha de Marajó (PA)

Lack of routine surveillance in countries endemic for bovine tuberculosis (TB) and limited laboratory support contributes to the inability to differentiate the Mycobacterium tuberculosis Complex species, leading to an underestimated burden of the disease. Here, Whole-Genome Sequencing of Mycobacterium bovis isolated from tissues with TB-like lesions obtained from cattle and buffalos at Marajó Island, Brazil, demonstrates that recent transmission of M. bovis is ongoing at distinct sites. Moreover, the M. bovis epidemiology in this setting is herein found to be dominated by an endemic and unique clade composed of strains evolved from a common ancestor that are now genetically differentiated from other M. bovis clades. Additionally, envisioning a rapid strain differentiation and tracing across multiple settings, 28 globally validated strain-specific SNPs were identified, three of which considered as robust markers for the M. bovis Marajó strain. In conclusion, this study contributes with data regarding the identification of a novel M. bovis phylogenetic clade responsible for ongoing transmission events in both cattle and buffalo species in Brazil, provides a framework to investigate the dissemination of this highly prevalent strain and, holds the potential to inform TB control strategies that may help to prevent the spread of bovine and zoonotic TB.