HPV-associated penile cancer: impact of copy number alterations in miRNA/mRNA interactions and potential druggable targets

Detalhes bibliográficos
Autor(a) principal: Silva, Jenilson da
Data de Publicação: 2021
Outros Autores: Nogueira, Leudivan, Coelho, Ronald, Deus, Amanda, Khayat, André, Marchi, Rafael, Oliveira, Edivaldo Herculano Corrêa de, Santos, Ana Paula dos, Cavalli, Luciane, Pereira, Silma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4459
Resumo: BACKGROUND: Penile cancer (PeCa) is a rare disease, but its incidence has increased worldwide, mostly in HPV+ patients. Nevertheless, there is still no targeted treatment for this carcinoma. OBJECTIVE: To predict the main signaling pathways involved in penile tumorigenesis and its potential drug targets. METHODS: Genome-wide copy number profiling was performed in 28 PeCa. Integration analysis of CNAs and miRNAs and mRNA targets was performed by DIANA-TarBase v.8. The potential impact of the miRNAs/target genes on biological pathways was assessed by DIANA-miRPath v.3.0. For each miRNA, KEGG pathways were generated based on the tarbase and microT-CDS algorithms. Pharmaco-miR was used to identify associations between miRNAs and their target genes to predict druggable targets. RESULTS: 269 miRNAs and 2,395 genes were mapped in cytobands with CNAs. The comparison of the miRNAs mapped at these cytobands and the miRNAs that were predicted to regulate the genes also mapped in these regions, resulted in a set of common 35 miRNAs and 292 genes. Enrichment pathway revealed their involvement in five top signaling pathways. EGFR and COX2 were identified as potential druggable targets. CONCLUSION: Our data indicate the potential use of EGFR and COX2 inhibitors as a target treatment for PeCa patients.
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spelling Silva, Jenilson daNogueira, LeudivanCoelho, RonaldDeus, AmandaKhayat, AndréMarchi, RafaelOliveira, Edivaldo Herculano Corrêa deSantos, Ana Paula dosCavalli, LucianePereira, Silma2021-10-19T14:20:58Z2021-10-19T14:20:58Z2021SILVA, Jenilson da et al. HPV-associated penile cancer: impact of copy number alterations in miRNA/mRNA interactions and potential druggable targets. Cancer Biomarkers, v. 32, n. 2, p. 147-160, 2021.1574-0153https://patua.iec.gov.br/handle/iec/445910.3233/CBM-210035.BACKGROUND: Penile cancer (PeCa) is a rare disease, but its incidence has increased worldwide, mostly in HPV+ patients. Nevertheless, there is still no targeted treatment for this carcinoma. OBJECTIVE: To predict the main signaling pathways involved in penile tumorigenesis and its potential drug targets. METHODS: Genome-wide copy number profiling was performed in 28 PeCa. Integration analysis of CNAs and miRNAs and mRNA targets was performed by DIANA-TarBase v.8. The potential impact of the miRNAs/target genes on biological pathways was assessed by DIANA-miRPath v.3.0. For each miRNA, KEGG pathways were generated based on the tarbase and microT-CDS algorithms. Pharmaco-miR was used to identify associations between miRNAs and their target genes to predict druggable targets. RESULTS: 269 miRNAs and 2,395 genes were mapped in cytobands with CNAs. The comparison of the miRNAs mapped at these cytobands and the miRNAs that were predicted to regulate the genes also mapped in these regions, resulted in a set of common 35 miRNAs and 292 genes. Enrichment pathway revealed their involvement in five top signaling pathways. EGFR and COX2 were identified as potential druggable targets. CONCLUSION: Our data indicate the potential use of EGFR and COX2 inhibitors as a target treatment for PeCa patients.Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Federal University of Maranhão. Postgraduate Program in Health Science. São Luís, MA, Brazil.Federal University of Maranhão. Postgraduate Program in Health Science. São Luís, MA, Brazil / Aldenora Bello Cancer Hospital. São Luís, MA, Brazil.Aldenora Bello Cancer Hospital. São Luís, MA, Brazil.Federal University of Maranhão. Postgraduate Program in Health Science. São Luís, MA, Brazil / Aldenora Bello Cancer Hospital. São Luís, MA, Brazil.Federal University of Pará. Oncology Research Center. Belém, PA, Brazil.Faculdades Pequeno Príncipe. Research Institute Pelé Pequeno Príncipe. Curitiba, PR, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil.Federal University of Maranhão. Department of Physiological Sciences. São Luís, MA, Brazil.Faculdades Pequeno Príncipe. Research Institute Pelé Pequeno Príncipe. Curitiba, PR, Brazil.Federal University of Maranhão. Department of Biology. Laboratory of Genetics and Molecular Biology. 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dc.title.pt_BR.fl_str_mv HPV-associated penile cancer: impact of copy number alterations in miRNA/mRNA interactions and potential druggable targets
title HPV-associated penile cancer: impact of copy number alterations in miRNA/mRNA interactions and potential druggable targets
spellingShingle HPV-associated penile cancer: impact of copy number alterations in miRNA/mRNA interactions and potential druggable targets
Silva, Jenilson da
Pênis / anatomia & histologia
Neoplasias Penianas / patologia
Biomarcadores Tumorais
Papillomaviridae / patogenicidade
Carcinogênese / genética
title_short HPV-associated penile cancer: impact of copy number alterations in miRNA/mRNA interactions and potential druggable targets
title_full HPV-associated penile cancer: impact of copy number alterations in miRNA/mRNA interactions and potential druggable targets
title_fullStr HPV-associated penile cancer: impact of copy number alterations in miRNA/mRNA interactions and potential druggable targets
title_full_unstemmed HPV-associated penile cancer: impact of copy number alterations in miRNA/mRNA interactions and potential druggable targets
title_sort HPV-associated penile cancer: impact of copy number alterations in miRNA/mRNA interactions and potential druggable targets
author Silva, Jenilson da
author_facet Silva, Jenilson da
Nogueira, Leudivan
Coelho, Ronald
Deus, Amanda
Khayat, André
Marchi, Rafael
Oliveira, Edivaldo Herculano Corrêa de
Santos, Ana Paula dos
Cavalli, Luciane
Pereira, Silma
author_role author
author2 Nogueira, Leudivan
Coelho, Ronald
Deus, Amanda
Khayat, André
Marchi, Rafael
Oliveira, Edivaldo Herculano Corrêa de
Santos, Ana Paula dos
Cavalli, Luciane
Pereira, Silma
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Jenilson da
Nogueira, Leudivan
Coelho, Ronald
Deus, Amanda
Khayat, André
Marchi, Rafael
Oliveira, Edivaldo Herculano Corrêa de
Santos, Ana Paula dos
Cavalli, Luciane
Pereira, Silma
dc.subject.decsPrimary.pt_BR.fl_str_mv Pênis / anatomia & histologia
Neoplasias Penianas / patologia
Biomarcadores Tumorais
Papillomaviridae / patogenicidade
Carcinogênese / genética
topic Pênis / anatomia & histologia
Neoplasias Penianas / patologia
Biomarcadores Tumorais
Papillomaviridae / patogenicidade
Carcinogênese / genética
description BACKGROUND: Penile cancer (PeCa) is a rare disease, but its incidence has increased worldwide, mostly in HPV+ patients. Nevertheless, there is still no targeted treatment for this carcinoma. OBJECTIVE: To predict the main signaling pathways involved in penile tumorigenesis and its potential drug targets. METHODS: Genome-wide copy number profiling was performed in 28 PeCa. Integration analysis of CNAs and miRNAs and mRNA targets was performed by DIANA-TarBase v.8. The potential impact of the miRNAs/target genes on biological pathways was assessed by DIANA-miRPath v.3.0. For each miRNA, KEGG pathways were generated based on the tarbase and microT-CDS algorithms. Pharmaco-miR was used to identify associations between miRNAs and their target genes to predict druggable targets. RESULTS: 269 miRNAs and 2,395 genes were mapped in cytobands with CNAs. The comparison of the miRNAs mapped at these cytobands and the miRNAs that were predicted to regulate the genes also mapped in these regions, resulted in a set of common 35 miRNAs and 292 genes. Enrichment pathway revealed their involvement in five top signaling pathways. EGFR and COX2 were identified as potential druggable targets. CONCLUSION: Our data indicate the potential use of EGFR and COX2 inhibitors as a target treatment for PeCa patients.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-10-19T14:20:58Z
dc.date.available.fl_str_mv 2021-10-19T14:20:58Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv SILVA, Jenilson da et al. HPV-associated penile cancer: impact of copy number alterations in miRNA/mRNA interactions and potential druggable targets. Cancer Biomarkers, v. 32, n. 2, p. 147-160, 2021.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4459
dc.identifier.issn.-.fl_str_mv 1574-0153
dc.identifier.doi.-.fl_str_mv 10.3233/CBM-210035.
identifier_str_mv SILVA, Jenilson da et al. HPV-associated penile cancer: impact of copy number alterations in miRNA/mRNA interactions and potential druggable targets. Cancer Biomarkers, v. 32, n. 2, p. 147-160, 2021.
1574-0153
10.3233/CBM-210035.
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