EVALUATION OF RESISTANCE TO BETA-LACTAM ANTIMICROBIALS IN STRAINS OF WILD ESCHERICHIA COLI, BW535 MUTANT OF CLINICAL ORIGIN CHALLENGED WITH MENADIONE
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Health and Society |
Texto Completo: | https://www.periodicojs.com.br/index.php/hs/article/view/1288 |
Resumo: | The Enterobacteriaceae family is one of the main groups of infectious agents that presents mechanisms of resistance to antimicrobials, including broad-spectrum ones such as carbapenem beta-lactams. Previous studies have reported the possibility that oxidative stress, which means the imbalance between the oxidative challenge and the body’s antioxidant defense capacity, is aligned with antimicrobial resistance. The body has a complex system of antioxidant protection, as a defense mechanism against free radicals, which are constantly formed in normal cellular metabolism and in various pathogenic events and, when in excess, can cause the oxidation of biological molecules. Free radicals whose unpaired electron is centered on oxygen or nitrogen atoms are called ROS (reactive oxygen species and ERNs (reactive nitrogen species) that can originate from exogenous oxidizing agents such as menadione. This quinone increases in cells the levels of ROS inducing the production of superoxide (SOD) and hydrogen peroxide (H2O2) which can cause cell death.However, bacteria have several defense mechanisms against ROS that play an important role in maintaining physiology. bacteria respond to oxidative stress with expression coordinated by multiple genes. In particular, the SoxRS regulons of Escherichia coli which are composed of genes that are encoded by products that provide resistance to stress to superoxide. In this work we will evaluate in strains of wild E.coli AB1157 , Base Excision Repair (BER) deficient mutant, and samples isolated from clinical material to possible resistance to antimicrobials and menadione. |
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EVALUATION OF RESISTANCE TO BETA-LACTAM ANTIMICROBIALS IN STRAINS OF WILD ESCHERICHIA COLI, BW535 MUTANT OF CLINICAL ORIGIN CHALLENGED WITH MENADIONEenterobacteria, oxidative stress, genesThe Enterobacteriaceae family is one of the main groups of infectious agents that presents mechanisms of resistance to antimicrobials, including broad-spectrum ones such as carbapenem beta-lactams. Previous studies have reported the possibility that oxidative stress, which means the imbalance between the oxidative challenge and the body’s antioxidant defense capacity, is aligned with antimicrobial resistance. The body has a complex system of antioxidant protection, as a defense mechanism against free radicals, which are constantly formed in normal cellular metabolism and in various pathogenic events and, when in excess, can cause the oxidation of biological molecules. Free radicals whose unpaired electron is centered on oxygen or nitrogen atoms are called ROS (reactive oxygen species and ERNs (reactive nitrogen species) that can originate from exogenous oxidizing agents such as menadione. This quinone increases in cells the levels of ROS inducing the production of superoxide (SOD) and hydrogen peroxide (H2O2) which can cause cell death.However, bacteria have several defense mechanisms against ROS that play an important role in maintaining physiology. bacteria respond to oxidative stress with expression coordinated by multiple genes. In particular, the SoxRS regulons of Escherichia coli which are composed of genes that are encoded by products that provide resistance to stress to superoxide. In this work we will evaluate in strains of wild E.coli AB1157 , Base Excision Repair (BER) deficient mutant, and samples isolated from clinical material to possible resistance to antimicrobials and menadione.Editora Acadêmica Periodicojs2023-05-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.periodicojs.com.br/index.php/hs/article/view/128810.51249/hs.v3i02.1288Health and Society; Vol. 3 No. 02 (2023); 69 - 91Health and Society; v. 3 n. 02 (2023); 69 - 912763-572410.51249/hs.v3i02reponame:Health and Societyinstname:Instituto de Ensino e Pesquisa Periodicojsinstacron:IEPPporhttps://www.periodicojs.com.br/index.php/hs/article/view/1288/1128Copyright (c) 2023 Juliana Graça dos Santos, Alexandre Ribeiro Bello, Ana Cláudia de Paula Ignáciohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGraça dos Santos, Juliana Ribeiro Bello, Alexandre Cláudia de Paula Ignácio, Ana 2023-05-05T11:42:13Zoai:ojs.pkp.sfu.ca:article/1288Revistahttps://www.periodicojs.com.br/index.php/hs/PRIhttps://www.periodicojs.com.br/index.php/hs/oaijournalofhealthandsociety@gmail.com || periodicojs@gmail.com2763-57242763-5724opendoar:2023-05-05T11:42:13Health and Society - Instituto de Ensino e Pesquisa Periodicojsfalse |
dc.title.none.fl_str_mv |
EVALUATION OF RESISTANCE TO BETA-LACTAM ANTIMICROBIALS IN STRAINS OF WILD ESCHERICHIA COLI, BW535 MUTANT OF CLINICAL ORIGIN CHALLENGED WITH MENADIONE |
title |
EVALUATION OF RESISTANCE TO BETA-LACTAM ANTIMICROBIALS IN STRAINS OF WILD ESCHERICHIA COLI, BW535 MUTANT OF CLINICAL ORIGIN CHALLENGED WITH MENADIONE |
spellingShingle |
EVALUATION OF RESISTANCE TO BETA-LACTAM ANTIMICROBIALS IN STRAINS OF WILD ESCHERICHIA COLI, BW535 MUTANT OF CLINICAL ORIGIN CHALLENGED WITH MENADIONE Graça dos Santos, Juliana enterobacteria, oxidative stress, genes |
title_short |
EVALUATION OF RESISTANCE TO BETA-LACTAM ANTIMICROBIALS IN STRAINS OF WILD ESCHERICHIA COLI, BW535 MUTANT OF CLINICAL ORIGIN CHALLENGED WITH MENADIONE |
title_full |
EVALUATION OF RESISTANCE TO BETA-LACTAM ANTIMICROBIALS IN STRAINS OF WILD ESCHERICHIA COLI, BW535 MUTANT OF CLINICAL ORIGIN CHALLENGED WITH MENADIONE |
title_fullStr |
EVALUATION OF RESISTANCE TO BETA-LACTAM ANTIMICROBIALS IN STRAINS OF WILD ESCHERICHIA COLI, BW535 MUTANT OF CLINICAL ORIGIN CHALLENGED WITH MENADIONE |
title_full_unstemmed |
EVALUATION OF RESISTANCE TO BETA-LACTAM ANTIMICROBIALS IN STRAINS OF WILD ESCHERICHIA COLI, BW535 MUTANT OF CLINICAL ORIGIN CHALLENGED WITH MENADIONE |
title_sort |
EVALUATION OF RESISTANCE TO BETA-LACTAM ANTIMICROBIALS IN STRAINS OF WILD ESCHERICHIA COLI, BW535 MUTANT OF CLINICAL ORIGIN CHALLENGED WITH MENADIONE |
author |
Graça dos Santos, Juliana |
author_facet |
Graça dos Santos, Juliana Ribeiro Bello, Alexandre Cláudia de Paula Ignácio, Ana |
author_role |
author |
author2 |
Ribeiro Bello, Alexandre Cláudia de Paula Ignácio, Ana |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Graça dos Santos, Juliana Ribeiro Bello, Alexandre Cláudia de Paula Ignácio, Ana |
dc.subject.por.fl_str_mv |
enterobacteria, oxidative stress, genes |
topic |
enterobacteria, oxidative stress, genes |
description |
The Enterobacteriaceae family is one of the main groups of infectious agents that presents mechanisms of resistance to antimicrobials, including broad-spectrum ones such as carbapenem beta-lactams. Previous studies have reported the possibility that oxidative stress, which means the imbalance between the oxidative challenge and the body’s antioxidant defense capacity, is aligned with antimicrobial resistance. The body has a complex system of antioxidant protection, as a defense mechanism against free radicals, which are constantly formed in normal cellular metabolism and in various pathogenic events and, when in excess, can cause the oxidation of biological molecules. Free radicals whose unpaired electron is centered on oxygen or nitrogen atoms are called ROS (reactive oxygen species and ERNs (reactive nitrogen species) that can originate from exogenous oxidizing agents such as menadione. This quinone increases in cells the levels of ROS inducing the production of superoxide (SOD) and hydrogen peroxide (H2O2) which can cause cell death.However, bacteria have several defense mechanisms against ROS that play an important role in maintaining physiology. bacteria respond to oxidative stress with expression coordinated by multiple genes. In particular, the SoxRS regulons of Escherichia coli which are composed of genes that are encoded by products that provide resistance to stress to superoxide. In this work we will evaluate in strains of wild E.coli AB1157 , Base Excision Repair (BER) deficient mutant, and samples isolated from clinical material to possible resistance to antimicrobials and menadione. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.periodicojs.com.br/index.php/hs/article/view/1288 10.51249/hs.v3i02.1288 |
url |
https://www.periodicojs.com.br/index.php/hs/article/view/1288 |
identifier_str_mv |
10.51249/hs.v3i02.1288 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.periodicojs.com.br/index.php/hs/article/view/1288/1128 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Editora Acadêmica Periodicojs |
publisher.none.fl_str_mv |
Editora Acadêmica Periodicojs |
dc.source.none.fl_str_mv |
Health and Society; Vol. 3 No. 02 (2023); 69 - 91 Health and Society; v. 3 n. 02 (2023); 69 - 91 2763-5724 10.51249/hs.v3i02 reponame:Health and Society instname:Instituto de Ensino e Pesquisa Periodicojs instacron:IEPP |
instname_str |
Instituto de Ensino e Pesquisa Periodicojs |
instacron_str |
IEPP |
institution |
IEPP |
reponame_str |
Health and Society |
collection |
Health and Society |
repository.name.fl_str_mv |
Health and Society - Instituto de Ensino e Pesquisa Periodicojs |
repository.mail.fl_str_mv |
journalofhealthandsociety@gmail.com || periodicojs@gmail.com |
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1797047894527705088 |