Characterization and selection of ISSR molecular markers in species of Passiflora spp.

• Autor(a) principal: Dias, Nátilla Deyse Souza Costa
• Data de Publicação: 2020
• Outros Autores: Souza, Larissa Neres Barbosa de, Santana, Vandrick de Oliveira, Silveira, Lucas Amorim, Meira, Messulan Rodrigues, Santos, Elisa Susilene Lisboa dos, Faleiro, Fábio Gelape, Silva, Carlos Bernard Moreno Cerqueira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Multi-Science Journal
• Texto Completo: https://periodicos.ifgoiano.edu.br/multiscience/article/view/1290
• topic: Diversity; passion fruit; genetic improvement; marker selection

The objective to be achieved with the search for molecular markers of low cost and good reproducibility for the selection and genetic characterization of passion fruit was to select ISSR (Inter Simple Sequence Repeat) primers for different unimproved varieties and cultivars of Passiflora, in order to subsidize molecular genetic studies. To achieve this goal, 23 ISSR starters were tested for eight unimproved varieties and 12 cultivars of Passiflora spp. The amplifications were obtained by electrophoresis on 2% agarose gel, photocumented under transilluminator with ultraviolet light. The ISSR primers showed satisfactory results. Among the 23 primers, 16 obtained values above 60% of efficiency when classified as good (presence of two marks or more). As a consequence of the results presented, it can be attested that such primers are suitable for studies of polymorphism, structure and genetic diversity in species of the Passiflora genus, in addition to assisting conservation and breeding programs.