EFFECTS OF THE INCREASE IN SUBSTRATE LOAD AND HYDROLYSIS TIME IN THE SACCHARIFICATION OF SUGARCANE BAGASSE AND ETHANOL PRODUCTION
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Holos |
Texto Completo: | http://www2.ifrn.edu.br/ojs/index.php/HOLOS/article/view/9485 |
Resumo: | In this study, the enzymatic saccharification of alkaline pretreated sugarcane bagasse (PSB) and the production of second generation bioethanol were investigated under different conditions. The effects of percentage of dry substrate (3 and 10% w/v) and time of hydrolysis (24 and 72 h) were evaluated in order to obtain maximum contents of total reducing sugar (TRS), glucose and xylose. The monosaccharide concentrations were quantified by High Performance Liquid Chromatography (HPLC) and TRS by the 3.5-dinitrosalicylic acid (DNS) method. The saccharification of PSB with the Cellic Ctec3, promoted an efficient release of sugars. Among the four evaluated conditions, the hydrolysis HD (10% PSB and 72h) was selected as the most efficient with the highest concentrations of released sugars: TRS (73.96 g/L), glucose (31.78 g/L) and xylose (10.85 g/L). The alcoholic fermentation of hydrolysate HD with the yeast Saccharomyces cerevisiae produced the maximum concentration of 16.15 g/L of ethanol, with a volumetric productivity (QP) of 0.69 g/Lh and yield of ethanol as a function of the substrate (YP/S) of 0.48 gethanol/gsugar. S. cerevisiae was capable of fermenting 99.96% of glucose from hydrolysate HD. These results suggest that the increase of the substrate load and time in the enzymatic hydrolysis can be favourable alternatives to improve the sugar yields in hydrolysates and, consequently, the bioethanol production. |
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EFFECTS OF THE INCREASE IN SUBSTRATE LOAD AND HYDROLYSIS TIME IN THE SACCHARIFICATION OF SUGARCANE BAGASSE AND ETHANOL PRODUCTIONResíduos agrícolasLignoceluloseBioetanolIn this study, the enzymatic saccharification of alkaline pretreated sugarcane bagasse (PSB) and the production of second generation bioethanol were investigated under different conditions. The effects of percentage of dry substrate (3 and 10% w/v) and time of hydrolysis (24 and 72 h) were evaluated in order to obtain maximum contents of total reducing sugar (TRS), glucose and xylose. The monosaccharide concentrations were quantified by High Performance Liquid Chromatography (HPLC) and TRS by the 3.5-dinitrosalicylic acid (DNS) method. The saccharification of PSB with the Cellic Ctec3, promoted an efficient release of sugars. Among the four evaluated conditions, the hydrolysis HD (10% PSB and 72h) was selected as the most efficient with the highest concentrations of released sugars: TRS (73.96 g/L), glucose (31.78 g/L) and xylose (10.85 g/L). The alcoholic fermentation of hydrolysate HD with the yeast Saccharomyces cerevisiae produced the maximum concentration of 16.15 g/L of ethanol, with a volumetric productivity (QP) of 0.69 g/Lh and yield of ethanol as a function of the substrate (YP/S) of 0.48 gethanol/gsugar. S. cerevisiae was capable of fermenting 99.96% of glucose from hydrolysate HD. These results suggest that the increase of the substrate load and time in the enzymatic hydrolysis can be favourable alternatives to improve the sugar yields in hydrolysates and, consequently, the bioethanol production.Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte2020-12-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www2.ifrn.edu.br/ojs/index.php/HOLOS/article/view/948510.15628/holos.2020.9485HOLOS; v. 6 (2020); 1-131807-1600reponame:Holosinstname:Instituto Federal do Rio Grande do Norte (IFRN)instacron:IFRNenghttp://www2.ifrn.edu.br/ojs/index.php/HOLOS/article/view/9485/pdfCopyright (c) 2020 HOLOSinfo:eu-repo/semantics/openAccessLamounier, Kenia FPasquini, DanielRodrigues, Patrisia OSoares, Alexandre SBaffi, Milla A2022-05-01T19:15:44Zoai:holos.ifrn.edu.br:article/9485Revistahttp://www2.ifrn.edu.br/ojs/index.php/HOLOSPUBhttp://www2.ifrn.edu.br/ojs/index.php/HOLOS/oaiholos@ifrn.edu.br||jyp.leite@ifrn.edu.br||propi@ifrn.edu.br1807-16001518-1634opendoar:2022-05-01T19:15:44Holos - Instituto Federal do Rio Grande do Norte (IFRN)false |
dc.title.none.fl_str_mv |
EFFECTS OF THE INCREASE IN SUBSTRATE LOAD AND HYDROLYSIS TIME IN THE SACCHARIFICATION OF SUGARCANE BAGASSE AND ETHANOL PRODUCTION |
title |
EFFECTS OF THE INCREASE IN SUBSTRATE LOAD AND HYDROLYSIS TIME IN THE SACCHARIFICATION OF SUGARCANE BAGASSE AND ETHANOL PRODUCTION |
spellingShingle |
EFFECTS OF THE INCREASE IN SUBSTRATE LOAD AND HYDROLYSIS TIME IN THE SACCHARIFICATION OF SUGARCANE BAGASSE AND ETHANOL PRODUCTION Lamounier, Kenia F Resíduos agrícolas Lignocelulose Bioetanol |
title_short |
EFFECTS OF THE INCREASE IN SUBSTRATE LOAD AND HYDROLYSIS TIME IN THE SACCHARIFICATION OF SUGARCANE BAGASSE AND ETHANOL PRODUCTION |
title_full |
EFFECTS OF THE INCREASE IN SUBSTRATE LOAD AND HYDROLYSIS TIME IN THE SACCHARIFICATION OF SUGARCANE BAGASSE AND ETHANOL PRODUCTION |
title_fullStr |
EFFECTS OF THE INCREASE IN SUBSTRATE LOAD AND HYDROLYSIS TIME IN THE SACCHARIFICATION OF SUGARCANE BAGASSE AND ETHANOL PRODUCTION |
title_full_unstemmed |
EFFECTS OF THE INCREASE IN SUBSTRATE LOAD AND HYDROLYSIS TIME IN THE SACCHARIFICATION OF SUGARCANE BAGASSE AND ETHANOL PRODUCTION |
title_sort |
EFFECTS OF THE INCREASE IN SUBSTRATE LOAD AND HYDROLYSIS TIME IN THE SACCHARIFICATION OF SUGARCANE BAGASSE AND ETHANOL PRODUCTION |
author |
Lamounier, Kenia F |
author_facet |
Lamounier, Kenia F Pasquini, Daniel Rodrigues, Patrisia O Soares, Alexandre S Baffi, Milla A |
author_role |
author |
author2 |
Pasquini, Daniel Rodrigues, Patrisia O Soares, Alexandre S Baffi, Milla A |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Lamounier, Kenia F Pasquini, Daniel Rodrigues, Patrisia O Soares, Alexandre S Baffi, Milla A |
dc.subject.por.fl_str_mv |
Resíduos agrícolas Lignocelulose Bioetanol |
topic |
Resíduos agrícolas Lignocelulose Bioetanol |
description |
In this study, the enzymatic saccharification of alkaline pretreated sugarcane bagasse (PSB) and the production of second generation bioethanol were investigated under different conditions. The effects of percentage of dry substrate (3 and 10% w/v) and time of hydrolysis (24 and 72 h) were evaluated in order to obtain maximum contents of total reducing sugar (TRS), glucose and xylose. The monosaccharide concentrations were quantified by High Performance Liquid Chromatography (HPLC) and TRS by the 3.5-dinitrosalicylic acid (DNS) method. The saccharification of PSB with the Cellic Ctec3, promoted an efficient release of sugars. Among the four evaluated conditions, the hydrolysis HD (10% PSB and 72h) was selected as the most efficient with the highest concentrations of released sugars: TRS (73.96 g/L), glucose (31.78 g/L) and xylose (10.85 g/L). The alcoholic fermentation of hydrolysate HD with the yeast Saccharomyces cerevisiae produced the maximum concentration of 16.15 g/L of ethanol, with a volumetric productivity (QP) of 0.69 g/Lh and yield of ethanol as a function of the substrate (YP/S) of 0.48 gethanol/gsugar. S. cerevisiae was capable of fermenting 99.96% of glucose from hydrolysate HD. These results suggest that the increase of the substrate load and time in the enzymatic hydrolysis can be favourable alternatives to improve the sugar yields in hydrolysates and, consequently, the bioethanol production. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www2.ifrn.edu.br/ojs/index.php/HOLOS/article/view/9485 10.15628/holos.2020.9485 |
url |
http://www2.ifrn.edu.br/ojs/index.php/HOLOS/article/view/9485 |
identifier_str_mv |
10.15628/holos.2020.9485 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www2.ifrn.edu.br/ojs/index.php/HOLOS/article/view/9485/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 HOLOS info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 HOLOS |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte |
publisher.none.fl_str_mv |
Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Norte |
dc.source.none.fl_str_mv |
HOLOS; v. 6 (2020); 1-13 1807-1600 reponame:Holos instname:Instituto Federal do Rio Grande do Norte (IFRN) instacron:IFRN |
instname_str |
Instituto Federal do Rio Grande do Norte (IFRN) |
instacron_str |
IFRN |
institution |
IFRN |
reponame_str |
Holos |
collection |
Holos |
repository.name.fl_str_mv |
Holos - Instituto Federal do Rio Grande do Norte (IFRN) |
repository.mail.fl_str_mv |
holos@ifrn.edu.br||jyp.leite@ifrn.edu.br||propi@ifrn.edu.br |
_version_ |
1798951625128148992 |