The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes

Detalhes bibliográficos
Autor(a) principal: Fernandes,I. M.
Data de Publicação: 2017
Outros Autores: Bastos,Y. F., Barreto,D. S., Lourenço,L. S., Penha,J. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842017000300444
Resumo: Abstract Clove oil is used as a fish anesthetic because it is a natural and inexpensive product with low toxicity risks. The goal of the present study was to determine the appropriate concentration of clove oil for small-sized tropical fish to be used in mark-recapture studies or when individuals are to be sacrificed. We applied three different clove oil concentrations (D1=0.05 mL, D2=0.10 mL and D3=0.20 mL per 500 mL of water) on three small-sized fish species. We found a negative relationship between induction time and treatment for two species (Hyphessobrycon sp.1 and Hemigrammus sp.), while concentration was unrelated to recovery time. Fish body length was positively related to induction time in the D2 treatment for Hemigrammus sp., and negatively for Hyphessobrycon sp.1 in the D1 treatment, but was unrelated to recovery time for three species and treatments. Mortality rates varied across treatments, but higher rates were observed with higher clove oil concentrations. We conclude that 0.05 mL of clove oil per 500 mL of water is the most efficient dose for studies where fish will be released back to their natural habitats, while 0.20 mL of clove oil is recommended for studies that require fish euthanization for further laboratory analyses.
id IIE-1_0cadb6617149d2007bd9bc207cd1c615
oai_identifier_str oai:scielo:S1519-69842017000300444
network_acronym_str IIE-1
network_name_str Brazilian Journal of Biology
repository_id_str
spelling The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishesEugenolanesthesiaeuthanasiafield experimentfish manipulationAbstract Clove oil is used as a fish anesthetic because it is a natural and inexpensive product with low toxicity risks. The goal of the present study was to determine the appropriate concentration of clove oil for small-sized tropical fish to be used in mark-recapture studies or when individuals are to be sacrificed. We applied three different clove oil concentrations (D1=0.05 mL, D2=0.10 mL and D3=0.20 mL per 500 mL of water) on three small-sized fish species. We found a negative relationship between induction time and treatment for two species (Hyphessobrycon sp.1 and Hemigrammus sp.), while concentration was unrelated to recovery time. Fish body length was positively related to induction time in the D2 treatment for Hemigrammus sp., and negatively for Hyphessobrycon sp.1 in the D1 treatment, but was unrelated to recovery time for three species and treatments. Mortality rates varied across treatments, but higher rates were observed with higher clove oil concentrations. We conclude that 0.05 mL of clove oil per 500 mL of water is the most efficient dose for studies where fish will be released back to their natural habitats, while 0.20 mL of clove oil is recommended for studies that require fish euthanization for further laboratory analyses.Instituto Internacional de Ecologia2017-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842017000300444Brazilian Journal of Biology v.77 n.3 2017reponame:Brazilian Journal of Biologyinstname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/1519-6984.15015info:eu-repo/semantics/openAccessFernandes,I. M.Bastos,Y. F.Barreto,D. S.Lourenço,L. S.Penha,J. M.eng2017-07-18T00:00:00Zoai:scielo:S1519-69842017000300444Revistahttps://www.scielo.br/j/bjb/https://old.scielo.br/oai/scielo-oai.phpbjb@bjb.com.br||bjb@bjb.com.br1678-43751519-6984opendoar:2017-07-18T00:00Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)false
dc.title.none.fl_str_mv The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes
title The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes
spellingShingle The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes
Fernandes,I. M.
Eugenol
anesthesia
euthanasia
field experiment
fish manipulation
title_short The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes
title_full The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes
title_fullStr The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes
title_full_unstemmed The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes
title_sort The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes
author Fernandes,I. M.
author_facet Fernandes,I. M.
Bastos,Y. F.
Barreto,D. S.
Lourenço,L. S.
Penha,J. M.
author_role author
author2 Bastos,Y. F.
Barreto,D. S.
Lourenço,L. S.
Penha,J. M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Fernandes,I. M.
Bastos,Y. F.
Barreto,D. S.
Lourenço,L. S.
Penha,J. M.
dc.subject.por.fl_str_mv Eugenol
anesthesia
euthanasia
field experiment
fish manipulation
topic Eugenol
anesthesia
euthanasia
field experiment
fish manipulation
description Abstract Clove oil is used as a fish anesthetic because it is a natural and inexpensive product with low toxicity risks. The goal of the present study was to determine the appropriate concentration of clove oil for small-sized tropical fish to be used in mark-recapture studies or when individuals are to be sacrificed. We applied three different clove oil concentrations (D1=0.05 mL, D2=0.10 mL and D3=0.20 mL per 500 mL of water) on three small-sized fish species. We found a negative relationship between induction time and treatment for two species (Hyphessobrycon sp.1 and Hemigrammus sp.), while concentration was unrelated to recovery time. Fish body length was positively related to induction time in the D2 treatment for Hemigrammus sp., and negatively for Hyphessobrycon sp.1 in the D1 treatment, but was unrelated to recovery time for three species and treatments. Mortality rates varied across treatments, but higher rates were observed with higher clove oil concentrations. We conclude that 0.05 mL of clove oil per 500 mL of water is the most efficient dose for studies where fish will be released back to their natural habitats, while 0.20 mL of clove oil is recommended for studies that require fish euthanization for further laboratory analyses.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842017000300444
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842017000300444
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1519-6984.15015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Internacional de Ecologia
publisher.none.fl_str_mv Instituto Internacional de Ecologia
dc.source.none.fl_str_mv Brazilian Journal of Biology v.77 n.3 2017
reponame:Brazilian Journal of Biology
instname:Instituto Internacional de Ecologia (IIE)
instacron:IIE
instname_str Instituto Internacional de Ecologia (IIE)
instacron_str IIE
institution IIE
reponame_str Brazilian Journal of Biology
collection Brazilian Journal of Biology
repository.name.fl_str_mv Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)
repository.mail.fl_str_mv bjb@bjb.com.br||bjb@bjb.com.br
_version_ 1752129883789590528