Genetic characterization of the peptidases of Polistes versicolor (Hymenoptera: Vespidae)

Detalhes bibliográficos
Autor(a) principal: Del Lama,M. A.
Data de Publicação: 2003
Outros Autores: Ferreira,K. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842003000200014
Resumo: Starch gel electrophoresis with L-leucyl-beta-naphthylamide as substrate revealed five aminopeptidases in extracts of Polistes versicolor. These enzymes are presumably products of five structural gene loci. All but Lap¹ aminopeptidases exhibited differential distribution in the developmental stages and in the tissues. Five dipeptidases were revealed with different dipeptides. These enzymes exhibited significant differences in their substrate preferences, but a more homogeneous distribution throughout ontogenetic developmental stages than did aminopeptidases. Electrophoretic variants of Lap4 and PepA² were detected and although a low intralocus heterozygosity was found due to the low frequency of these variants, phenotypical segregation observed at these loci in pupae extracts of some colonies points to the occurrence of more than one egg-laying female. Otherwise, the detection of Lap4 allozyme restricted to nests of one area suggests low dispersion ability of the adults of Polistes versicolor.
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spelling Genetic characterization of the peptidases of Polistes versicolor (Hymenoptera: Vespidae)peptidasessubstrate preferencedevelopmental distributiongenetic variationPolistes versicolorStarch gel electrophoresis with L-leucyl-beta-naphthylamide as substrate revealed five aminopeptidases in extracts of Polistes versicolor. These enzymes are presumably products of five structural gene loci. All but Lap¹ aminopeptidases exhibited differential distribution in the developmental stages and in the tissues. Five dipeptidases were revealed with different dipeptides. These enzymes exhibited significant differences in their substrate preferences, but a more homogeneous distribution throughout ontogenetic developmental stages than did aminopeptidases. Electrophoretic variants of Lap4 and PepA² were detected and although a low intralocus heterozygosity was found due to the low frequency of these variants, phenotypical segregation observed at these loci in pupae extracts of some colonies points to the occurrence of more than one egg-laying female. Otherwise, the detection of Lap4 allozyme restricted to nests of one area suggests low dispersion ability of the adults of Polistes versicolor.Instituto Internacional de Ecologia2003-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842003000200014Brazilian Journal of Biology v.63 n.2 2003reponame:Brazilian Journal of Biologyinstname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/S1519-69842003000200014info:eu-repo/semantics/openAccessDel Lama,M. A.Ferreira,K. M.eng2003-08-15T00:00:00Zoai:scielo:S1519-69842003000200014Revistahttps://www.scielo.br/j/bjb/https://old.scielo.br/oai/scielo-oai.phpbjb@bjb.com.br||bjb@bjb.com.br1678-43751519-6984opendoar:2003-08-15T00:00Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)false
dc.title.none.fl_str_mv Genetic characterization of the peptidases of Polistes versicolor (Hymenoptera: Vespidae)
title Genetic characterization of the peptidases of Polistes versicolor (Hymenoptera: Vespidae)
spellingShingle Genetic characterization of the peptidases of Polistes versicolor (Hymenoptera: Vespidae)
Del Lama,M. A.
peptidases
substrate preference
developmental distribution
genetic variation
Polistes versicolor
title_short Genetic characterization of the peptidases of Polistes versicolor (Hymenoptera: Vespidae)
title_full Genetic characterization of the peptidases of Polistes versicolor (Hymenoptera: Vespidae)
title_fullStr Genetic characterization of the peptidases of Polistes versicolor (Hymenoptera: Vespidae)
title_full_unstemmed Genetic characterization of the peptidases of Polistes versicolor (Hymenoptera: Vespidae)
title_sort Genetic characterization of the peptidases of Polistes versicolor (Hymenoptera: Vespidae)
author Del Lama,M. A.
author_facet Del Lama,M. A.
Ferreira,K. M.
author_role author
author2 Ferreira,K. M.
author2_role author
dc.contributor.author.fl_str_mv Del Lama,M. A.
Ferreira,K. M.
dc.subject.por.fl_str_mv peptidases
substrate preference
developmental distribution
genetic variation
Polistes versicolor
topic peptidases
substrate preference
developmental distribution
genetic variation
Polistes versicolor
description Starch gel electrophoresis with L-leucyl-beta-naphthylamide as substrate revealed five aminopeptidases in extracts of Polistes versicolor. These enzymes are presumably products of five structural gene loci. All but Lap¹ aminopeptidases exhibited differential distribution in the developmental stages and in the tissues. Five dipeptidases were revealed with different dipeptides. These enzymes exhibited significant differences in their substrate preferences, but a more homogeneous distribution throughout ontogenetic developmental stages than did aminopeptidases. Electrophoretic variants of Lap4 and PepA² were detected and although a low intralocus heterozygosity was found due to the low frequency of these variants, phenotypical segregation observed at these loci in pupae extracts of some colonies points to the occurrence of more than one egg-laying female. Otherwise, the detection of Lap4 allozyme restricted to nests of one area suggests low dispersion ability of the adults of Polistes versicolor.
publishDate 2003
dc.date.none.fl_str_mv 2003-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842003000200014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842003000200014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1519-69842003000200014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Internacional de Ecologia
publisher.none.fl_str_mv Instituto Internacional de Ecologia
dc.source.none.fl_str_mv Brazilian Journal of Biology v.63 n.2 2003
reponame:Brazilian Journal of Biology
instname:Instituto Internacional de Ecologia (IIE)
instacron:IIE
instname_str Instituto Internacional de Ecologia (IIE)
instacron_str IIE
institution IIE
reponame_str Brazilian Journal of Biology
collection Brazilian Journal of Biology
repository.name.fl_str_mv Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)
repository.mail.fl_str_mv bjb@bjb.com.br||bjb@bjb.com.br
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