Enzymatic analysis in Anopheles nuneztovari Gabaldón (Diptera, Culicidae)
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Biologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000400002 |
Resumo: | Enzymatic analysis in Anopheles nuneztovari was made using four populations from the Brazilian Amazon and two from Colombia. The enzymes ME and XDH presented a monomorphic locus in all of the studied populations. EST and LAP presented a higher number of loci. In EST, genetic variation was observed in the five loci; LAP presented four loci, with allec variation in two loci. In IDH, three activity regions were stained, with genetic variation for locus Idh-1 in the Brazilian Amazon populations. A locus for MDH was observed, with genetic variation in the six populations. A region was verified for ACON, with four alleles in Sitronela and three in the other populations. PGM constituted one locus, with a high variability in the Brazilian Amazon populations. A locus was observed for 6-PGD with allelic variation in all of the populations with the exception of Tibú. Enzyme PGI presented two loci, both with genetic variability in the Tucuruí population. The enzyme alpha-GPD showed an activity region with polymorphism in the Tucuruí, Tibú and Sitronela populations. The phenotypic variations detected for these enzymes suggest that four (EST, LAP, ACON and PGM) possess monomeric structures and five (IDH, MDH, 6-PGD, PGI and alpha-GPD) dimeric structures in their proteins. These enzymes constitute in important markers to estimate variability and genetic divergence in natural populations of A. nuneztovari. |
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Enzymatic analysis in Anopheles nuneztovari Gabaldón (Diptera, Culicidae)isozymeselectrophoretic profilesgenetic variationneotropical anophelineAmazonianEnzymatic analysis in Anopheles nuneztovari was made using four populations from the Brazilian Amazon and two from Colombia. The enzymes ME and XDH presented a monomorphic locus in all of the studied populations. EST and LAP presented a higher number of loci. In EST, genetic variation was observed in the five loci; LAP presented four loci, with allec variation in two loci. In IDH, three activity regions were stained, with genetic variation for locus Idh-1 in the Brazilian Amazon populations. A locus for MDH was observed, with genetic variation in the six populations. A region was verified for ACON, with four alleles in Sitronela and three in the other populations. PGM constituted one locus, with a high variability in the Brazilian Amazon populations. A locus was observed for 6-PGD with allelic variation in all of the populations with the exception of Tibú. Enzyme PGI presented two loci, both with genetic variability in the Tucuruí population. The enzyme alpha-GPD showed an activity region with polymorphism in the Tucuruí, Tibú and Sitronela populations. The phenotypic variations detected for these enzymes suggest that four (EST, LAP, ACON and PGM) possess monomeric structures and five (IDH, MDH, 6-PGD, PGI and alpha-GPD) dimeric structures in their proteins. These enzymes constitute in important markers to estimate variability and genetic divergence in natural populations of A. nuneztovari.Instituto Internacional de Ecologia2000-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000400002Revista Brasileira de Biologia v.60 n.4 2000reponame:Revista Brasileira de Biologia (Online)instname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/S0034-71082000000400002info:eu-repo/semantics/openAccessSCARPASSA,V. M.TADEI,W. P.eng2001-02-22T00:00:00Zoai:scielo:S0034-71082000000400002Revistawww.scielo.br/rbbioONGhttps://old.scielo.br/oai/scielo-oai.php||bjb.iie@terra.com.br1806-96060034-7108opendoar:2001-02-22T00:00Revista Brasileira de Biologia (Online) - Instituto Internacional de Ecologia (IIE)false |
dc.title.none.fl_str_mv |
Enzymatic analysis in Anopheles nuneztovari Gabaldón (Diptera, Culicidae) |
title |
Enzymatic analysis in Anopheles nuneztovari Gabaldón (Diptera, Culicidae) |
spellingShingle |
Enzymatic analysis in Anopheles nuneztovari Gabaldón (Diptera, Culicidae) SCARPASSA,V. M. isozymes electrophoretic profiles genetic variation neotropical anopheline Amazonian |
title_short |
Enzymatic analysis in Anopheles nuneztovari Gabaldón (Diptera, Culicidae) |
title_full |
Enzymatic analysis in Anopheles nuneztovari Gabaldón (Diptera, Culicidae) |
title_fullStr |
Enzymatic analysis in Anopheles nuneztovari Gabaldón (Diptera, Culicidae) |
title_full_unstemmed |
Enzymatic analysis in Anopheles nuneztovari Gabaldón (Diptera, Culicidae) |
title_sort |
Enzymatic analysis in Anopheles nuneztovari Gabaldón (Diptera, Culicidae) |
author |
SCARPASSA,V. M. |
author_facet |
SCARPASSA,V. M. TADEI,W. P. |
author_role |
author |
author2 |
TADEI,W. P. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
SCARPASSA,V. M. TADEI,W. P. |
dc.subject.por.fl_str_mv |
isozymes electrophoretic profiles genetic variation neotropical anopheline Amazonian |
topic |
isozymes electrophoretic profiles genetic variation neotropical anopheline Amazonian |
description |
Enzymatic analysis in Anopheles nuneztovari was made using four populations from the Brazilian Amazon and two from Colombia. The enzymes ME and XDH presented a monomorphic locus in all of the studied populations. EST and LAP presented a higher number of loci. In EST, genetic variation was observed in the five loci; LAP presented four loci, with allec variation in two loci. In IDH, three activity regions were stained, with genetic variation for locus Idh-1 in the Brazilian Amazon populations. A locus for MDH was observed, with genetic variation in the six populations. A region was verified for ACON, with four alleles in Sitronela and three in the other populations. PGM constituted one locus, with a high variability in the Brazilian Amazon populations. A locus was observed for 6-PGD with allelic variation in all of the populations with the exception of Tibú. Enzyme PGI presented two loci, both with genetic variability in the Tucuruí population. The enzyme alpha-GPD showed an activity region with polymorphism in the Tucuruí, Tibú and Sitronela populations. The phenotypic variations detected for these enzymes suggest that four (EST, LAP, ACON and PGM) possess monomeric structures and five (IDH, MDH, 6-PGD, PGI and alpha-GPD) dimeric structures in their proteins. These enzymes constitute in important markers to estimate variability and genetic divergence in natural populations of A. nuneztovari. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000400002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000400002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0034-71082000000400002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
publisher.none.fl_str_mv |
Instituto Internacional de Ecologia |
dc.source.none.fl_str_mv |
Revista Brasileira de Biologia v.60 n.4 2000 reponame:Revista Brasileira de Biologia (Online) instname:Instituto Internacional de Ecologia (IIE) instacron:IIE |
instname_str |
Instituto Internacional de Ecologia (IIE) |
instacron_str |
IIE |
institution |
IIE |
reponame_str |
Revista Brasileira de Biologia (Online) |
collection |
Revista Brasileira de Biologia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Biologia (Online) - Instituto Internacional de Ecologia (IIE) |
repository.mail.fl_str_mv |
||bjb.iie@terra.com.br |
_version_ |
1754821093760172033 |