Regulatory T cells isolated from endometriotic peritoneal fluid express a different number of Toll-like receptors

• Autor(a) principal: Hernandes,Camila
• Data de Publicação: 2020
• Outros Autores: Gueuvoghlanian-Silva,Bárbara Yasmin, Monnaka,Vitor Ulisses, Ribeiro,Natalia Mazini, Pereira,Welbert de Oliveira, Podgaec,Sérgio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Einstein (São Paulo)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082020000100247
• topic: T-lymphocytes, regulatory; Toll-like receptors; Immune system; Endometriosis

ABSTRACT Objective To analyze and compare the expression of Toll-like receptors by regulatory T cells present in the peritoneal fluid of patients with and without endometriosis. Methods Regulatory T cells were isolated from peritoneal fluid of women with and without endometriosis, collected during surgery, and mRNA was extracted for analysis of Toll-like receptors expression by reverse-transcriptase polymerase chain reaction. Results Patients with endometriosis presented regulatory T cells expressing a larger number and variety of Toll-like receptors when compared to regulatory T cells from patients in the Control Group. Toll-like receptor-1 and Toll-like receptor-2 in regulatory T cells were expressed in both groups. All other expressed Toll-like receptors types were only found in regulatory T cells from the Endometriosis Group. Conclusion Patients with endometriosis had peritoneal regulatory T cells expressing various Toll-like receptors types.