Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria

Detalhes bibliográficos
Autor(a) principal: Correia,Rodolfo Patussi
Data de Publicação: 2016
Outros Autores: Bento,Laiz Cameirão, Bortolucci,Ana Carolina Apelle, Alexandre,Anderson Marega, Vaz,Andressa da Costa, Schimidell,Daniela, Pedro,Eduardo de Carvalho, Perin,Fabricio Simões, Nozawa,Sonia Tsukasa, Mendes,Cláudio Ernesto Albers, Barroso,Rodrigo de Souza, Bacal,Nydia Strachman
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Einstein (São Paulo)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082016000300366
Resumo: ABSTRACT Objective: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. Methods: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. Results: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. Conclusion: High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones.
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spelling Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuriaHemoglobinuriaparoxysmal/diagnosisFlow cytometry/methodsTechnological developmentABSTRACT Objective: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. Methods: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. Results: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. Conclusion: High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones.Instituto Israelita de Ensino e Pesquisa Albert Einstein2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082016000300366einstein (São Paulo) v.14 n.3 2016reponame:Einstein (São Paulo)instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)instacron:IIEPAE10.1590/S1679-45082016AO3641info:eu-repo/semantics/openAccessCorreia,Rodolfo PatussiBento,Laiz CameirãoBortolucci,Ana Carolina ApelleAlexandre,Anderson MaregaVaz,Andressa da CostaSchimidell,DanielaPedro,Eduardo de CarvalhoPerin,Fabricio SimõesNozawa,Sonia TsukasaMendes,Cláudio Ernesto AlbersBarroso,Rodrigo de SouzaBacal,Nydia Strachmaneng2016-10-14T00:00:00Zoai:scielo:S1679-45082016000300366Revistahttps://journal.einstein.br/pt-br/ONGhttps://old.scielo.br/oai/scielo-oai.php||revista@einstein.br2317-63851679-4508opendoar:2016-10-14T00:00Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)false
dc.title.none.fl_str_mv Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria
title Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria
spellingShingle Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria
Correia,Rodolfo Patussi
Hemoglobinuria
paroxysmal/diagnosis
Flow cytometry/methods
Technological development
title_short Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria
title_full Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria
title_fullStr Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria
title_full_unstemmed Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria
title_sort Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria
author Correia,Rodolfo Patussi
author_facet Correia,Rodolfo Patussi
Bento,Laiz Cameirão
Bortolucci,Ana Carolina Apelle
Alexandre,Anderson Marega
Vaz,Andressa da Costa
Schimidell,Daniela
Pedro,Eduardo de Carvalho
Perin,Fabricio Simões
Nozawa,Sonia Tsukasa
Mendes,Cláudio Ernesto Albers
Barroso,Rodrigo de Souza
Bacal,Nydia Strachman
author_role author
author2 Bento,Laiz Cameirão
Bortolucci,Ana Carolina Apelle
Alexandre,Anderson Marega
Vaz,Andressa da Costa
Schimidell,Daniela
Pedro,Eduardo de Carvalho
Perin,Fabricio Simões
Nozawa,Sonia Tsukasa
Mendes,Cláudio Ernesto Albers
Barroso,Rodrigo de Souza
Bacal,Nydia Strachman
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Correia,Rodolfo Patussi
Bento,Laiz Cameirão
Bortolucci,Ana Carolina Apelle
Alexandre,Anderson Marega
Vaz,Andressa da Costa
Schimidell,Daniela
Pedro,Eduardo de Carvalho
Perin,Fabricio Simões
Nozawa,Sonia Tsukasa
Mendes,Cláudio Ernesto Albers
Barroso,Rodrigo de Souza
Bacal,Nydia Strachman
dc.subject.por.fl_str_mv Hemoglobinuria
paroxysmal/diagnosis
Flow cytometry/methods
Technological development
topic Hemoglobinuria
paroxysmal/diagnosis
Flow cytometry/methods
Technological development
description ABSTRACT Objective: To discuss the implementation of technical advances in laboratory diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria for validation of high-sensitivity flow cytometry protocols. Methods: A retrospective study based on analysis of laboratory data from 745 patient samples submitted to flow cytometry for diagnosis and/or monitoring of paroxysmal nocturnal hemoglobinuria. Results: Implementation of technical advances reduced test costs and improved flow cytometry resolution for paroxysmal nocturnal hemoglobinuria clone detection. Conclusion: High-sensitivity flow cytometry allowed more sensitive determination of paroxysmal nocturnal hemoglobinuria clone type and size, particularly in samples with small clones.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082016000300366
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082016000300366
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1679-45082016AO3641
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Israelita de Ensino e Pesquisa Albert Einstein
publisher.none.fl_str_mv Instituto Israelita de Ensino e Pesquisa Albert Einstein
dc.source.none.fl_str_mv einstein (São Paulo) v.14 n.3 2016
reponame:Einstein (São Paulo)
instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)
instacron:IIEPAE
instname_str Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)
instacron_str IIEPAE
institution IIEPAE
reponame_str Einstein (São Paulo)
collection Einstein (São Paulo)
repository.name.fl_str_mv Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)
repository.mail.fl_str_mv ||revista@einstein.br
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