Reduction of oxidative stress improves insulin signaling in cardiac tissue of obese mice

• Autor(a) principal: Rodrigues,Matheus Scarpatto
• Data de Publicação: 2020
• Outros Autores: Pieri,Bruno Luiz da Silva, Silveira,Gustavo de Bem, Zaccaron,Rubya Pereira, Venturini,Ligia Milanez, Comin,Vitor Hugo, Luiz,Karine Damian, Silveira,Paulo Cesar Lock
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Einstein (São Paulo)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082020000100246
• topic: Obesity; Insulin resistance; Oxidative stress; Myocardium; Mice

ABSTRACT Objective To evaluate the effects of oxidative stress on insulin signaling in cardiac tissue of obese mice. Methods Thirty Swiss mice were equally divided (n=10) into three groups: Control Group, Obese Group, and Obese Group Treated with N-acetylcysteine. After obesity and insulin resistance were established, the obese mice were treated with N-acetylcysteine at a dose of 50mg/kg daily for 15 days via oral gavage. Results Higher blood glucose levels and nitrite and carbonyl contents, and lower protein levels of glutathione peroxidase and phosphorylated protein kinase B were observed in the obese group when compared with their respective control. On the other hand, treatment with N-acetylcysteine was effective in reducing blood glucose levels and nitrite and carbonyl contents, and significantly increased protein levels of glutathione peroxidase and phosphorylated protein kinase B compared to the Obese Group. Conclusion Obesity and/or a high-lipid diet may result in oxidative stress and insulin resistance in the heart tissue of obese mice, and the use of N-acetylcysteine as a methodological and therapeutic strategy suggested there is a relation between them.