Manutenção de tatus em cativeiro e resultados de inoculação do Mycobacterium Leprae

Detalhes bibliográficos
Autor(a) principal: OPROMOLLA, Diltor Vladimir Araújo
Data de Publicação: 1980
Outros Autores: ARRUDA, Olavo Speranza de, FLEURY, Raul Negrão
Tipo de documento: Artigo
Idioma: por
Título da fonte: Hansenologia Internationalis (Online)
Texto Completo: https://periodicos.saude.sp.gov.br/hansenologia/article/view/35616
Resumo: The authors report their experiences in raising arm ldillos and the results obtained in inoculating them with M. leprae. They begin by reporting on the origin of the animals, how they were captured, and their efforts until they found the right kind of housing for the armadillos. They also tell of their attempts to develop the correct feeding procedures. The result is the feed used now in their laboratory: chicken feed mixed with bread, eggs, meat and milk. Along with this, they report on the way each animal is registered and the examination it undergoes upon admittance, namely, recording of its weight, rectal temperature, feces examination, hemogram and hemossedimentation. They also tell how animals are immobilized so as to take blood samples for the inoculations. Lastly, they present the results of the inoculations with M. leprae in three armadillos of the Euphractus sexticinctus species and 26 of the Dasypus novemcinctus species, with special reference to an armadillo of the D. novemcinctus species, which had systemic infection 15 months after being inoculated subcutaneously and intravenously. Bacilli were found in large quantities in various organs like the skin, lymph nodes, liver, kidneys and spleen. No bacilli were found in the myocardium, pancreas, thyroid and testicles. The authors are of the opinion that this is the first armadillo of the D. novemcinctus species to present experimental systemic infection with M. leprae in South America. Another armadillo, which has been inoculated with a suspension of 1.2 x 108 bacilli by the intracardiac route already showed nodules with bacilli on the abdominal wall 10 months after being inoculated. They believe that the results already obtained indicate that soon they will be able to provide material for institutions in Brazil as well as other countries that are interested in the study of M. leprae and in the preparation of the Mitsuda antigen which is so vital to the control of Hansen's disease.
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spelling Manutenção de tatus em cativeiro e resultados de inoculação do Mycobacterium LepraeTatuMycobacterium lepraeInoculaçãoArmadilloMycobacterium lepraeInoculationThe authors report their experiences in raising arm ldillos and the results obtained in inoculating them with M. leprae. They begin by reporting on the origin of the animals, how they were captured, and their efforts until they found the right kind of housing for the armadillos. They also tell of their attempts to develop the correct feeding procedures. The result is the feed used now in their laboratory: chicken feed mixed with bread, eggs, meat and milk. Along with this, they report on the way each animal is registered and the examination it undergoes upon admittance, namely, recording of its weight, rectal temperature, feces examination, hemogram and hemossedimentation. They also tell how animals are immobilized so as to take blood samples for the inoculations. Lastly, they present the results of the inoculations with M. leprae in three armadillos of the Euphractus sexticinctus species and 26 of the Dasypus novemcinctus species, with special reference to an armadillo of the D. novemcinctus species, which had systemic infection 15 months after being inoculated subcutaneously and intravenously. Bacilli were found in large quantities in various organs like the skin, lymph nodes, liver, kidneys and spleen. No bacilli were found in the myocardium, pancreas, thyroid and testicles. The authors are of the opinion that this is the first armadillo of the D. novemcinctus species to present experimental systemic infection with M. leprae in South America. Another armadillo, which has been inoculated with a suspension of 1.2 x 108 bacilli by the intracardiac route already showed nodules with bacilli on the abdominal wall 10 months after being inoculated. They believe that the results already obtained indicate that soon they will be able to provide material for institutions in Brazil as well as other countries that are interested in the study of M. leprae and in the preparation of the Mitsuda antigen which is so vital to the control of Hansen's disease.Os autores relatam sua experiência na manutenção de tatus em cativeiro e apresentam os resultados da inoculação do M. leprae. Inicialmente fazem considerações sobre a proveniência dos animais, como é processada sua captura e as tentativas que realizaram até conseguirem as instalações atuais para albergá-los. Relatam ainda as experiências feitas para a padronização de uma ração que fosse adequada e o resultado a que chegaram, que consiste de uma ração para frangos misturada a pão, ovos, carne e leite. Além disso, discorrem sobre o registro e o exame dos animais, que se constitui principalmente em um exame físico do animal na entrada, verificação do peso e temperatura retal e a realização de exame de fezes, hemograma e homossedimentação, e sobre como os animais são imobilizados para a colheita do sangue e para as inoculações. Finalmente, apresentam os seus resultados da inoculação do M. leprae em 3 animais da espécie Euphractus sexticinctus e em 26 animais da espécie Dasypus novemcinctus com referência especial a um D. novemcinctus (n.° 19) que apresentou infecção sistêmica após 15 meses de inoculação por via subcutânea e intravenosa. Bacilos foram encontrados em grande quantidade em vários órgãos como pele, linfonodos, fígado, supra-renais e baço. Não foram encontrados bacilos no miocárdio, pâncreas, tireóide e testículos. Ao que parece, este constitui o primeiro animal da espécie D. novemcinctus que apresentou infecção experimental sistêmica com o M. leprae na América do Sul. Um outro tatu (n.° 11), inoculado com suspensão de 1,2 x 10e bacilos por via intracardíaca, já apresenta nódulos com bacilos na parede abdominal após 10 meses de inoculação. Concluem considerando que os resultados já conseguidos darão em breve condições para fornecerem material para as instituições existentes no País ou outras que se interessarem no estudo do M. leprae e para o preparo de antígeno de Missuda tão carente nos programas de controle de hanseníase.Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo1980-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/hansenologia/article/view/3561610.47878/hi.1980.v5.35616Hansenologia Internationalis: leprosy and other infectious diseases; Vol. 5 No. 1 (1980); 28-36Hansenologia Internationalis: hanseníase e outras doenças infecciosas; v. 5 n. 1 (1980); 28-361982-5161reponame:Hansenologia Internationalis (Online)instname:Instituto Lauro de Souza Lima (ILSL)instacron:ILSLporhttps://periodicos.saude.sp.gov.br/hansenologia/article/view/35616/34018OPROMOLLA, Diltor Vladimir AraújoARRUDA, Olavo Speranza deFLEURY, Raul Negrãoinfo:eu-repo/semantics/openAccess2021-06-06T05:18:51Zoai:ojs.periodicos.saude.sp.gov.br:article/35616Revistahttps://periodicos.saude.sp.gov.br/hansenologiaPRIhttps://periodicos.saude.sp.gov.br/hansenologia/oaihansen_int@ilsl.br || hansenologia.internationalis@gmail.com || periodicossp@saude.sp.gov.br1982-51610100-3283opendoar:2021-06-06T05:18:51Hansenologia Internationalis (Online) - Instituto Lauro de Souza Lima (ILSL)false
dc.title.none.fl_str_mv Manutenção de tatus em cativeiro e resultados de inoculação do Mycobacterium Leprae
title Manutenção de tatus em cativeiro e resultados de inoculação do Mycobacterium Leprae
spellingShingle Manutenção de tatus em cativeiro e resultados de inoculação do Mycobacterium Leprae
OPROMOLLA, Diltor Vladimir Araújo
Tatu
Mycobacterium leprae
Inoculação
Armadillo
Mycobacterium leprae
Inoculation
title_short Manutenção de tatus em cativeiro e resultados de inoculação do Mycobacterium Leprae
title_full Manutenção de tatus em cativeiro e resultados de inoculação do Mycobacterium Leprae
title_fullStr Manutenção de tatus em cativeiro e resultados de inoculação do Mycobacterium Leprae
title_full_unstemmed Manutenção de tatus em cativeiro e resultados de inoculação do Mycobacterium Leprae
title_sort Manutenção de tatus em cativeiro e resultados de inoculação do Mycobacterium Leprae
author OPROMOLLA, Diltor Vladimir Araújo
author_facet OPROMOLLA, Diltor Vladimir Araújo
ARRUDA, Olavo Speranza de
FLEURY, Raul Negrão
author_role author
author2 ARRUDA, Olavo Speranza de
FLEURY, Raul Negrão
author2_role author
author
dc.contributor.author.fl_str_mv OPROMOLLA, Diltor Vladimir Araújo
ARRUDA, Olavo Speranza de
FLEURY, Raul Negrão
dc.subject.por.fl_str_mv Tatu
Mycobacterium leprae
Inoculação
Armadillo
Mycobacterium leprae
Inoculation
topic Tatu
Mycobacterium leprae
Inoculação
Armadillo
Mycobacterium leprae
Inoculation
description The authors report their experiences in raising arm ldillos and the results obtained in inoculating them with M. leprae. They begin by reporting on the origin of the animals, how they were captured, and their efforts until they found the right kind of housing for the armadillos. They also tell of their attempts to develop the correct feeding procedures. The result is the feed used now in their laboratory: chicken feed mixed with bread, eggs, meat and milk. Along with this, they report on the way each animal is registered and the examination it undergoes upon admittance, namely, recording of its weight, rectal temperature, feces examination, hemogram and hemossedimentation. They also tell how animals are immobilized so as to take blood samples for the inoculations. Lastly, they present the results of the inoculations with M. leprae in three armadillos of the Euphractus sexticinctus species and 26 of the Dasypus novemcinctus species, with special reference to an armadillo of the D. novemcinctus species, which had systemic infection 15 months after being inoculated subcutaneously and intravenously. Bacilli were found in large quantities in various organs like the skin, lymph nodes, liver, kidneys and spleen. No bacilli were found in the myocardium, pancreas, thyroid and testicles. The authors are of the opinion that this is the first armadillo of the D. novemcinctus species to present experimental systemic infection with M. leprae in South America. Another armadillo, which has been inoculated with a suspension of 1.2 x 108 bacilli by the intracardiac route already showed nodules with bacilli on the abdominal wall 10 months after being inoculated. They believe that the results already obtained indicate that soon they will be able to provide material for institutions in Brazil as well as other countries that are interested in the study of M. leprae and in the preparation of the Mitsuda antigen which is so vital to the control of Hansen's disease.
publishDate 1980
dc.date.none.fl_str_mv 1980-06-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/hansenologia/article/view/35616
10.47878/hi.1980.v5.35616
url https://periodicos.saude.sp.gov.br/hansenologia/article/view/35616
identifier_str_mv 10.47878/hi.1980.v5.35616
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/hansenologia/article/view/35616/34018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo
publisher.none.fl_str_mv Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo
dc.source.none.fl_str_mv Hansenologia Internationalis: leprosy and other infectious diseases; Vol. 5 No. 1 (1980); 28-36
Hansenologia Internationalis: hanseníase e outras doenças infecciosas; v. 5 n. 1 (1980); 28-36
1982-5161
reponame:Hansenologia Internationalis (Online)
instname:Instituto Lauro de Souza Lima (ILSL)
instacron:ILSL
instname_str Instituto Lauro de Souza Lima (ILSL)
instacron_str ILSL
institution ILSL
reponame_str Hansenologia Internationalis (Online)
collection Hansenologia Internationalis (Online)
repository.name.fl_str_mv Hansenologia Internationalis (Online) - Instituto Lauro de Souza Lima (ILSL)
repository.mail.fl_str_mv hansen_int@ilsl.br || hansenologia.internationalis@gmail.com || periodicossp@saude.sp.gov.br
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