Murine experimental Hansen's disease: intranasal inoculation of the Mycobacterium leprae: inoculação do Mycobaterium leprae via intranasal

Detalhes bibliográficos
Autor(a) principal: Vilani-Moreno, Fatima Regina
Data de Publicação: 1999
Outros Autores: Arruda, Maria Sueli Parreira de, Nogueira, Maria Esther Salles, Baptista, Ida Maria Foschiani Dias
Tipo de documento: Artigo
Idioma: por
Título da fonte: Hansenologia Internationalis (Online)
Texto Completo: https://periodicos.saude.sp.gov.br/hansenologia/article/view/36424
Resumo: Considering the upper airways the most likely infective route for the M. leprae, the present study evaluated the behavior of the bacilli experimentally inoculated via intranasal. Thus, Swiss mice were inoculatedwith M. leprae by nasal instillation and then sacrificed in different periods of time. Lungs were removed and submitted to bronchoalveolar lavage (BAL) and histopathological exam in each sacrifice. The number ofbacilli recovered and the total and differential number of inflammatory cells were evaluated in the BAL. The BAL was also cultured in Lowenstein-Jensen and Middlebrook 7H9 and 7H11 media. Bacilli were found in the BAL until the 6th month after inoculation. Cultures for mycobacteria were all negative. The total number of cells, lymphocytes and neutrophyls in the BAL of infected mice was higher than in the control group until the 6th month of experimentation. The histopathological analy-sis showed small cellular infiltrates distributed throughout the pulmonary parenchyma and around vessels, up to the 6th month of sacrifice. Lesions of dissemination were. not found. The results obtained revealed that inoculation of M. leprae via intranasal in Swiss mice did not result in development of disease and neither in multiplication of the bacilli. Therefore, additional studies involving other strains of mice would be useful to determine the importance of the intranasal via in leprosy infection.
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spelling Murine experimental Hansen's disease: intranasal inoculation of the Mycobacterium leprae: inoculação do Mycobaterium leprae via intranasalHanseníase experimental murina: : inoculação do Mycobaterium leprae via intranasalHanseniaseCamundongoVia intranasalLavado broncoalveolarLeprosyMiceIntranasal viaBronchoalveolar lavageConsidering the upper airways the most likely infective route for the M. leprae, the present study evaluated the behavior of the bacilli experimentally inoculated via intranasal. Thus, Swiss mice were inoculatedwith M. leprae by nasal instillation and then sacrificed in different periods of time. Lungs were removed and submitted to bronchoalveolar lavage (BAL) and histopathological exam in each sacrifice. The number ofbacilli recovered and the total and differential number of inflammatory cells were evaluated in the BAL. The BAL was also cultured in Lowenstein-Jensen and Middlebrook 7H9 and 7H11 media. Bacilli were found in the BAL until the 6th month after inoculation. Cultures for mycobacteria were all negative. The total number of cells, lymphocytes and neutrophyls in the BAL of infected mice was higher than in the control group until the 6th month of experimentation. The histopathological analy-sis showed small cellular infiltrates distributed throughout the pulmonary parenchyma and around vessels, up to the 6th month of sacrifice. Lesions of dissemination were. not found. The results obtained revealed that inoculation of M. leprae via intranasal in Swiss mice did not result in development of disease and neither in multiplication of the bacilli. Therefore, additional studies involving other strains of mice would be useful to determine the importance of the intranasal via in leprosy infection.Considerando as vias aereas superiores como a mais provavel porta de entrada do M. teprae no organismo, no presente estudo avaliou-se 0 comportamento do bacilo inoculado experimentalmente via intranasal. Assim, camundongos Suiços foram inoculados por instilação nasal com M. teprae e sacrificados em diferentes periodos de tempo. Em cada sacrificio os pulmoes foram removidos e submetidos ao lavado broncoalveolar (LBA) e analise histopatológica. 0 LBA foi avaliado quanto ao numero de bacilos recuperados e numero total e diferencial de celulas inflamatórias; foi ainda semeado em meios de cultivo Lowenstein-Jensen e Middlebrook 7H9 e 7Hll. No LBA foram encontrados bacilos ate 0 6Q mes de inoculação; nao houve crescimento de micobacterias nas culturas. 0 numero total de celulas, linfócitos e neutrófilos do LBA obtido de camundongos infectados foi maior que o do grupo controle ate 0 6Q mes da experimentação. Aanalise histopatológica revelou pequenos infiltrados celulares distribuidos pelo parenquima pulmonar e ao redor de vasos ate 0 62 mes de sacrificio; nao foram observadas lesoes de disseminação. Os resultados obtidos revelam que a inoculação de M. leprae, via intranasal, em camundongos Suiços, nao levou ao desenvolvimento da doença nem a multiplicação dos bacilos; assim estudos adicionais envolvendo outras linhagens de camundongos poderao ser uteis para determinar a importancia da via intranasal na infecção hansenica.Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo1999-11-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/hansenologia/article/view/3642410.47878/hi.1999.v24.36424Hansenologia Internationalis: leprosy and other infectious diseases; Vol. 24 No. 2 (1999); 115-120Hansenologia Internationalis: hanseníase e outras doenças infecciosas; v. 24 n. 2 (1999); 115-1201982-5161reponame:Hansenologia Internationalis (Online)instname:Instituto Lauro de Souza Lima (ILSL)instacron:ILSLporhttps://periodicos.saude.sp.gov.br/hansenologia/article/view/36424/34701https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessVilani-Moreno, Fatima ReginaArruda, Maria Sueli Parreira deNogueira, Maria Esther SallesBaptista, Ida Maria Foschiani Dias2023-09-27T14:31:49Zoai:ojs.periodicos.saude.sp.gov.br:article/36424Revistahttps://periodicos.saude.sp.gov.br/hansenologiaPRIhttps://periodicos.saude.sp.gov.br/hansenologia/oaihansen_int@ilsl.br || hansenologia.internationalis@gmail.com || periodicossp@saude.sp.gov.br1982-51610100-3283opendoar:2023-09-27T14:31:49Hansenologia Internationalis (Online) - Instituto Lauro de Souza Lima (ILSL)false
dc.title.none.fl_str_mv Murine experimental Hansen's disease: intranasal inoculation of the Mycobacterium leprae: inoculação do Mycobaterium leprae via intranasal
Hanseníase experimental murina: : inoculação do Mycobaterium leprae via intranasal
title Murine experimental Hansen's disease: intranasal inoculation of the Mycobacterium leprae: inoculação do Mycobaterium leprae via intranasal
spellingShingle Murine experimental Hansen's disease: intranasal inoculation of the Mycobacterium leprae: inoculação do Mycobaterium leprae via intranasal
Vilani-Moreno, Fatima Regina
Hanseniase
Camundongo
Via intranasal
Lavado broncoalveolar
Leprosy
Mice
Intranasal via
Bronchoalveolar lavage
title_short Murine experimental Hansen's disease: intranasal inoculation of the Mycobacterium leprae: inoculação do Mycobaterium leprae via intranasal
title_full Murine experimental Hansen's disease: intranasal inoculation of the Mycobacterium leprae: inoculação do Mycobaterium leprae via intranasal
title_fullStr Murine experimental Hansen's disease: intranasal inoculation of the Mycobacterium leprae: inoculação do Mycobaterium leprae via intranasal
title_full_unstemmed Murine experimental Hansen's disease: intranasal inoculation of the Mycobacterium leprae: inoculação do Mycobaterium leprae via intranasal
title_sort Murine experimental Hansen's disease: intranasal inoculation of the Mycobacterium leprae: inoculação do Mycobaterium leprae via intranasal
author Vilani-Moreno, Fatima Regina
author_facet Vilani-Moreno, Fatima Regina
Arruda, Maria Sueli Parreira de
Nogueira, Maria Esther Salles
Baptista, Ida Maria Foschiani Dias
author_role author
author2 Arruda, Maria Sueli Parreira de
Nogueira, Maria Esther Salles
Baptista, Ida Maria Foschiani Dias
author2_role author
author
author
dc.contributor.author.fl_str_mv Vilani-Moreno, Fatima Regina
Arruda, Maria Sueli Parreira de
Nogueira, Maria Esther Salles
Baptista, Ida Maria Foschiani Dias
dc.subject.por.fl_str_mv Hanseniase
Camundongo
Via intranasal
Lavado broncoalveolar
Leprosy
Mice
Intranasal via
Bronchoalveolar lavage
topic Hanseniase
Camundongo
Via intranasal
Lavado broncoalveolar
Leprosy
Mice
Intranasal via
Bronchoalveolar lavage
description Considering the upper airways the most likely infective route for the M. leprae, the present study evaluated the behavior of the bacilli experimentally inoculated via intranasal. Thus, Swiss mice were inoculatedwith M. leprae by nasal instillation and then sacrificed in different periods of time. Lungs were removed and submitted to bronchoalveolar lavage (BAL) and histopathological exam in each sacrifice. The number ofbacilli recovered and the total and differential number of inflammatory cells were evaluated in the BAL. The BAL was also cultured in Lowenstein-Jensen and Middlebrook 7H9 and 7H11 media. Bacilli were found in the BAL until the 6th month after inoculation. Cultures for mycobacteria were all negative. The total number of cells, lymphocytes and neutrophyls in the BAL of infected mice was higher than in the control group until the 6th month of experimentation. The histopathological analy-sis showed small cellular infiltrates distributed throughout the pulmonary parenchyma and around vessels, up to the 6th month of sacrifice. Lesions of dissemination were. not found. The results obtained revealed that inoculation of M. leprae via intranasal in Swiss mice did not result in development of disease and neither in multiplication of the bacilli. Therefore, additional studies involving other strains of mice would be useful to determine the importance of the intranasal via in leprosy infection.
publishDate 1999
dc.date.none.fl_str_mv 1999-11-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/hansenologia/article/view/36424
10.47878/hi.1999.v24.36424
url https://periodicos.saude.sp.gov.br/hansenologia/article/view/36424
identifier_str_mv 10.47878/hi.1999.v24.36424
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/hansenologia/article/view/36424/34701
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo
publisher.none.fl_str_mv Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo
dc.source.none.fl_str_mv Hansenologia Internationalis: leprosy and other infectious diseases; Vol. 24 No. 2 (1999); 115-120
Hansenologia Internationalis: hanseníase e outras doenças infecciosas; v. 24 n. 2 (1999); 115-120
1982-5161
reponame:Hansenologia Internationalis (Online)
instname:Instituto Lauro de Souza Lima (ILSL)
instacron:ILSL
instname_str Instituto Lauro de Souza Lima (ILSL)
instacron_str ILSL
institution ILSL
reponame_str Hansenologia Internationalis (Online)
collection Hansenologia Internationalis (Online)
repository.name.fl_str_mv Hansenologia Internationalis (Online) - Instituto Lauro de Souza Lima (ILSL)
repository.mail.fl_str_mv hansen_int@ilsl.br || hansenologia.internationalis@gmail.com || periodicossp@saude.sp.gov.br
_version_ 1796797581404143616

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