Atividade da NADH-redutase de metemoglobina em hemolisado e membranas eritrocitárias de pacientes hansenianos sob tratamento sulfônico
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Hansenologia Internationalis (Online) |
Texto Completo: | https://periodicos.saude.sp.gov.br/hansenologia/article/view/36461 |
Resumo: | We measured in the blood samples of 72, adults leprosy patients, who were ingesting 100 mg of dapsone/day, the levels of erythrocytes, hemoglobin, methemoglobin, sulphone and reticulocytes. NADH-methemoglobin-reductase was measured both in the hemolysate and ghost cells. As a control, identical tests, except for sulphone measure, were applied to blood samples from 72 healthy individuals who did not ingest any oxidant drug. Significant statistical differences regarding the values in erythrocytes, methemoglobin, reticulocytes and activity of NADH- reductase in the erythrocytes ghosts were found among these two groups. The mean enzyme activity in the hemolysate of the leprosy patients and thehealthy individuals has not differed significantly when expressed in UI/g.Hemoglobin/l. The enzyme activity in erythrocytes ghosts from leprosy patients was significantly smaller then that in erythrocytes ghosts from healthy individuals. The mean level of methemoglobin in the leprosy patients was higher than in the control group, probably due to oxidizer action of sulphone. Tends in view we have found smaller levels of enzymatic activity in the erythrocytes membranes of the leprosy patients and coincident levels in the hemolysate compared to controls, we can affirm that probably this is due to an enzymatic displacement from the cellular membrane to the cytoplasm, with the objective of maintaining in the erythrocyte a constant hemoglobinic balance, in spite of the sulphone oxidizer action. |
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Atividade da NADH-redutase de metemoglobina em hemolisado e membranas eritrocitárias de pacientes hansenianos sob tratamento sulfônicoHanseníaseNADH-redutaseDiaforaseNADH-metemoglobina-redutaseLeprosyNADH- reductaseDiaphoraseNADH-methemoglobin-reductaseWe measured in the blood samples of 72, adults leprosy patients, who were ingesting 100 mg of dapsone/day, the levels of erythrocytes, hemoglobin, methemoglobin, sulphone and reticulocytes. NADH-methemoglobin-reductase was measured both in the hemolysate and ghost cells. As a control, identical tests, except for sulphone measure, were applied to blood samples from 72 healthy individuals who did not ingest any oxidant drug. Significant statistical differences regarding the values in erythrocytes, methemoglobin, reticulocytes and activity of NADH- reductase in the erythrocytes ghosts were found among these two groups. The mean enzyme activity in the hemolysate of the leprosy patients and thehealthy individuals has not differed significantly when expressed in UI/g.Hemoglobin/l. The enzyme activity in erythrocytes ghosts from leprosy patients was significantly smaller then that in erythrocytes ghosts from healthy individuals. The mean level of methemoglobin in the leprosy patients was higher than in the control group, probably due to oxidizer action of sulphone. Tends in view we have found smaller levels of enzymatic activity in the erythrocytes membranes of the leprosy patients and coincident levels in the hemolysate compared to controls, we can affirm that probably this is due to an enzymatic displacement from the cellular membrane to the cytoplasm, with the objective of maintaining in the erythrocyte a constant hemoglobinic balance, in spite of the sulphone oxidizer action.Nós efetuamos determinações da série vermelha, dosagem da sulfonemia, dosagem de metemoglobina, contagem de reticulócitos e dosagem da atividade enzimática da NADHdiaforase no hemolisado e nas membranas dos eritrócitos livres de hemoglobina em 72 pacientes portadores de hanseníase, todos ingerindo doses diárias de 100 mg de diaminodifenil-sulfona. Idênticos testes, exceto dosagem de sulfona, foram efetuados no sangue de 72 pessoas normais, não ingerindo medicamentos oxidantes. Foram encontradas diferenças estatísticas significativas nas variáveis da série vermelha, metemoglobina, reticulócitos e na atividade enzimática da NADH-redutase nas membranas eritrocitárias entre os dois grupos. A atividade enzimática nas membranas eritrocitárias foi inferior e estatisticamente significativa com relação ao grupo-controle. A atividade enzimática da NADH-redutase no hemolisado não apresentou diferença estatística significativa quando feita a correção pela taxa de hemoglobina. O nível de metemoglobina foi superior nos portadores de hanseníase em relação ao grupocontrole, provavelmente devido à ação oxidante da sulfona. Tendo em vista termos encontrado níveis inferiores de atividade enzimática nas membranas eritrocitárias dos hansenianos e níveiscoincidentes no hemolisado, em comparação com um grupo-controle, podemos afirmar que, provavelmente, isto se deve ao deslocamento enzimático da membrana celular para o citoplasma, com o objetivo de manter o eritrócito em equilíbrio hemoglobínico constante, apesar da ação oxidante da sulfona.Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo1998-11-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/hansenologia/article/view/3646110.47878/hi.1998.v23.36461Hansenologia Internationalis: leprosy and other infectious diseases; Vol. 23 No. 1/2 (1998); 14-26Hansenologia Internationalis: hanseníase e outras doenças infecciosas; v. 23 n. 1/2 (1998); 14-261982-5161reponame:Hansenologia Internationalis (Online)instname:Instituto Lauro de Souza Lima (ILSL)instacron:ILSLporhttps://periodicos.saude.sp.gov.br/hansenologia/article/view/36461/34740https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDalpino, DirceuDiltor, Luís Alberto MagnaOpromolla, Vladimir Araújo2023-10-10T13:34:11Zoai:ojs.periodicos.saude.sp.gov.br:article/36461Revistahttps://periodicos.saude.sp.gov.br/hansenologiaPRIhttps://periodicos.saude.sp.gov.br/hansenologia/oaihansen_int@ilsl.br || hansenologia.internationalis@gmail.com || periodicossp@saude.sp.gov.br1982-51610100-3283opendoar:2023-10-10T13:34:11Hansenologia Internationalis (Online) - Instituto Lauro de Souza Lima (ILSL)false |
dc.title.none.fl_str_mv |
Atividade da NADH-redutase de metemoglobina em hemolisado e membranas eritrocitárias de pacientes hansenianos sob tratamento sulfônico |
title |
Atividade da NADH-redutase de metemoglobina em hemolisado e membranas eritrocitárias de pacientes hansenianos sob tratamento sulfônico |
spellingShingle |
Atividade da NADH-redutase de metemoglobina em hemolisado e membranas eritrocitárias de pacientes hansenianos sob tratamento sulfônico Dalpino, Dirceu Hanseníase NADH-redutase Diaforase NADH-metemoglobina-redutase Leprosy NADH- reductase Diaphorase NADH-methemoglobin-reductase |
title_short |
Atividade da NADH-redutase de metemoglobina em hemolisado e membranas eritrocitárias de pacientes hansenianos sob tratamento sulfônico |
title_full |
Atividade da NADH-redutase de metemoglobina em hemolisado e membranas eritrocitárias de pacientes hansenianos sob tratamento sulfônico |
title_fullStr |
Atividade da NADH-redutase de metemoglobina em hemolisado e membranas eritrocitárias de pacientes hansenianos sob tratamento sulfônico |
title_full_unstemmed |
Atividade da NADH-redutase de metemoglobina em hemolisado e membranas eritrocitárias de pacientes hansenianos sob tratamento sulfônico |
title_sort |
Atividade da NADH-redutase de metemoglobina em hemolisado e membranas eritrocitárias de pacientes hansenianos sob tratamento sulfônico |
author |
Dalpino, Dirceu |
author_facet |
Dalpino, Dirceu Diltor, Luís Alberto Magna Opromolla, Vladimir Araújo |
author_role |
author |
author2 |
Diltor, Luís Alberto Magna Opromolla, Vladimir Araújo |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Dalpino, Dirceu Diltor, Luís Alberto Magna Opromolla, Vladimir Araújo |
dc.subject.por.fl_str_mv |
Hanseníase NADH-redutase Diaforase NADH-metemoglobina-redutase Leprosy NADH- reductase Diaphorase NADH-methemoglobin-reductase |
topic |
Hanseníase NADH-redutase Diaforase NADH-metemoglobina-redutase Leprosy NADH- reductase Diaphorase NADH-methemoglobin-reductase |
description |
We measured in the blood samples of 72, adults leprosy patients, who were ingesting 100 mg of dapsone/day, the levels of erythrocytes, hemoglobin, methemoglobin, sulphone and reticulocytes. NADH-methemoglobin-reductase was measured both in the hemolysate and ghost cells. As a control, identical tests, except for sulphone measure, were applied to blood samples from 72 healthy individuals who did not ingest any oxidant drug. Significant statistical differences regarding the values in erythrocytes, methemoglobin, reticulocytes and activity of NADH- reductase in the erythrocytes ghosts were found among these two groups. The mean enzyme activity in the hemolysate of the leprosy patients and thehealthy individuals has not differed significantly when expressed in UI/g.Hemoglobin/l. The enzyme activity in erythrocytes ghosts from leprosy patients was significantly smaller then that in erythrocytes ghosts from healthy individuals. The mean level of methemoglobin in the leprosy patients was higher than in the control group, probably due to oxidizer action of sulphone. Tends in view we have found smaller levels of enzymatic activity in the erythrocytes membranes of the leprosy patients and coincident levels in the hemolysate compared to controls, we can affirm that probably this is due to an enzymatic displacement from the cellular membrane to the cytoplasm, with the objective of maintaining in the erythrocyte a constant hemoglobinic balance, in spite of the sulphone oxidizer action. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-11-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.saude.sp.gov.br/hansenologia/article/view/36461 10.47878/hi.1998.v23.36461 |
url |
https://periodicos.saude.sp.gov.br/hansenologia/article/view/36461 |
identifier_str_mv |
10.47878/hi.1998.v23.36461 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.saude.sp.gov.br/hansenologia/article/view/36461/34740 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo |
publisher.none.fl_str_mv |
Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo |
dc.source.none.fl_str_mv |
Hansenologia Internationalis: leprosy and other infectious diseases; Vol. 23 No. 1/2 (1998); 14-26 Hansenologia Internationalis: hanseníase e outras doenças infecciosas; v. 23 n. 1/2 (1998); 14-26 1982-5161 reponame:Hansenologia Internationalis (Online) instname:Instituto Lauro de Souza Lima (ILSL) instacron:ILSL |
instname_str |
Instituto Lauro de Souza Lima (ILSL) |
instacron_str |
ILSL |
institution |
ILSL |
reponame_str |
Hansenologia Internationalis (Online) |
collection |
Hansenologia Internationalis (Online) |
repository.name.fl_str_mv |
Hansenologia Internationalis (Online) - Instituto Lauro de Souza Lima (ILSL) |
repository.mail.fl_str_mv |
hansen_int@ilsl.br || hansenologia.internationalis@gmail.com || periodicossp@saude.sp.gov.br |
_version_ |
1796797581463912448 |