Effect of reinfection on the evolution of rats infected with Trypanosoma cruzi
Autor(a) principal: | |
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Data de Publicação: | 1990 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | spa |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28743 |
Resumo: | The present study was undertaken in order to demonstrate that reinfection could modify parasitemia, serum antibodies, electrocardiographic patterns and/or myocardial lesions already observed in a rat model. Experimental groups IG: rats infected at weaning with 1 x 10(6) T. cruzi; RG: same as IG plus reinoculations each 30 days until completion on day 150; IG1: 51 day old infected rats; C: controls. A high parasitemia was detected in IG and RG until day 20 showing a tendency to become negative on day 30. No parasites were observed in RG after the first reinoculation which could not be attributed to the old age of the host since there was no parasitemia in IG1. Xenodiagnoses were negative in all three groups. Serum antibodies were not significantly modified in RG in comparison with IG, except for 7S antibodies, since RG showed higher titres in some days under study. No distinct patterns of basal ECG were observed in infected rats. The ajmalina test reduced the heart rate (HR) showing no treatment dependance. The PR, QaT and QRS were significantly lengthened in all groups regarding the basal one (p < 0.05), except for the QaT in C. Besides, the PR and QaT alterations were greater in the infected rats (p< 0.05). There was also present a wide variety of electric axis orientations, regarding the basal value, accompanied by morphological changes more evident in the RG. The incidence of cardiac lesions histologically detected in the infected group was significantly higher than in C (p < 0.01). It can be concluded that reinfection, neither produce the onset of acute initial disorders nor modify the type or degree of cardiac lesion histologically observed. The ajmaline test showed ECG alterations compatible with a mild myocardial damage in infected rats, whereas a major compromise was assessed in the reinfected group. |
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Effect of reinfection on the evolution of rats infected with Trypanosoma cruzi Efecto de la reinfección sobre la evolución de ratas infectadas con Trypanosoma cruzi T. cruzireinfección en ratasajmalina The present study was undertaken in order to demonstrate that reinfection could modify parasitemia, serum antibodies, electrocardiographic patterns and/or myocardial lesions already observed in a rat model. Experimental groups IG: rats infected at weaning with 1 x 10(6) T. cruzi; RG: same as IG plus reinoculations each 30 days until completion on day 150; IG1: 51 day old infected rats; C: controls. A high parasitemia was detected in IG and RG until day 20 showing a tendency to become negative on day 30. No parasites were observed in RG after the first reinoculation which could not be attributed to the old age of the host since there was no parasitemia in IG1. Xenodiagnoses were negative in all three groups. Serum antibodies were not significantly modified in RG in comparison with IG, except for 7S antibodies, since RG showed higher titres in some days under study. No distinct patterns of basal ECG were observed in infected rats. The ajmalina test reduced the heart rate (HR) showing no treatment dependance. The PR, QaT and QRS were significantly lengthened in all groups regarding the basal one (p < 0.05), except for the QaT in C. Besides, the PR and QaT alterations were greater in the infected rats (p< 0.05). There was also present a wide variety of electric axis orientations, regarding the basal value, accompanied by morphological changes more evident in the RG. The incidence of cardiac lesions histologically detected in the infected group was significantly higher than in C (p < 0.01). It can be concluded that reinfection, neither produce the onset of acute initial disorders nor modify the type or degree of cardiac lesion histologically observed. The ajmaline test showed ECG alterations compatible with a mild myocardial damage in infected rats, whereas a major compromise was assessed in the reinfected group. El objetivo de este trabajo fue comprobar si una de las variables medio-ambientales, la reinfección, puede modificar el comportamiento observado en un modelo de rata a nivel de parasitemia, anticuerpos séricos, manifestaciones electrocardiográficas y/o lesión miocárdica. Los grupos experimentales fueron: GI: ratas infectadas al destete con 1 x 10(6) T. cruzi; GR: igual a GI más reinfecciones cada 30 días hasta los 150 días post-infección inicial (p.i.i.); GI1 ratas de 51 días infectadas; GT: testigos. Se detectó parasitemia alta en GI y GR hasta los 20 días p.i.i. tendiendo a negativizarse al día 30. En GR no se observaron parásitos despúes del primer reinóculo, resistencia que no es debida sólo a la mayor edad del huésped pués hubo parasitemia en GI1. Los xenodiagnósticos fueron negativos en los tres grupos. Los anticuerpos séricos no se modificaron significativamente en GR respecto de GI, salvo en los anticuerpos 7S, pues los del GR presentaron títulos superiores en algunos de los días estudiados. Los ECG basales no mostraron cambios distintivos en las ratas infectadas. La prueba de ajmalina mostró una disminución de la FC independiente del tratamiento; el PR, QaT y QRS se prolongaron significativamente en todos los grupos respecto del basal (p < 0.05), salvo el QaT en el GT; además, el cambio de PR y QaT fue mayor en los infectados (p < 0.05). En los grupos infectados hubo también una amplia variación en la orientación del eje eléctrico respecto del valor basal, acompañado de cambios morfológicos más manifiestos en GR. La proporción de lesión cardíaca detectada histológicamente en los grupos infectados, fue significativamente superior a la del GT (p < 0.01). Se concluye que la reinfección no reproduce el cuadro agudo inicial y no modifica el tipo y grado de lesión cardíaca observada histológicamete. La prueba de ajmalina muestra cambios electrocardiográficos compatibles con daño miocárdico incipiente en las ratas infectadas y sugieren mayor compromiso en el grupo reinfectado. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1990-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28743Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 No. 4 (1990); 260-268 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 Núm. 4 (1990); 260-268 Revista do Instituto de Medicina Tropical de São Paulo; v. 32 n. 4 (1990); 260-268 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTspahttps://www.revistas.usp.br/rimtsp/article/view/28743/30596Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessRevelli, SilviaBerra, HéctorValenti, JoséMoreno, HildaBernasconi, MaríaPoli, HugoMorini, Julio2012-07-02T01:28:13Zoai:revistas.usp.br:article/28743Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:29.789019Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Effect of reinfection on the evolution of rats infected with Trypanosoma cruzi Efecto de la reinfección sobre la evolución de ratas infectadas con Trypanosoma cruzi |
title |
Effect of reinfection on the evolution of rats infected with Trypanosoma cruzi |
spellingShingle |
Effect of reinfection on the evolution of rats infected with Trypanosoma cruzi Revelli, Silvia T. cruzi reinfección en ratas ajmalina |
title_short |
Effect of reinfection on the evolution of rats infected with Trypanosoma cruzi |
title_full |
Effect of reinfection on the evolution of rats infected with Trypanosoma cruzi |
title_fullStr |
Effect of reinfection on the evolution of rats infected with Trypanosoma cruzi |
title_full_unstemmed |
Effect of reinfection on the evolution of rats infected with Trypanosoma cruzi |
title_sort |
Effect of reinfection on the evolution of rats infected with Trypanosoma cruzi |
author |
Revelli, Silvia |
author_facet |
Revelli, Silvia Berra, Héctor Valenti, José Moreno, Hilda Bernasconi, María Poli, Hugo Morini, Julio |
author_role |
author |
author2 |
Berra, Héctor Valenti, José Moreno, Hilda Bernasconi, María Poli, Hugo Morini, Julio |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Revelli, Silvia Berra, Héctor Valenti, José Moreno, Hilda Bernasconi, María Poli, Hugo Morini, Julio |
dc.subject.por.fl_str_mv |
T. cruzi reinfección en ratas ajmalina |
topic |
T. cruzi reinfección en ratas ajmalina |
description |
The present study was undertaken in order to demonstrate that reinfection could modify parasitemia, serum antibodies, electrocardiographic patterns and/or myocardial lesions already observed in a rat model. Experimental groups IG: rats infected at weaning with 1 x 10(6) T. cruzi; RG: same as IG plus reinoculations each 30 days until completion on day 150; IG1: 51 day old infected rats; C: controls. A high parasitemia was detected in IG and RG until day 20 showing a tendency to become negative on day 30. No parasites were observed in RG after the first reinoculation which could not be attributed to the old age of the host since there was no parasitemia in IG1. Xenodiagnoses were negative in all three groups. Serum antibodies were not significantly modified in RG in comparison with IG, except for 7S antibodies, since RG showed higher titres in some days under study. No distinct patterns of basal ECG were observed in infected rats. The ajmalina test reduced the heart rate (HR) showing no treatment dependance. The PR, QaT and QRS were significantly lengthened in all groups regarding the basal one (p < 0.05), except for the QaT in C. Besides, the PR and QaT alterations were greater in the infected rats (p< 0.05). There was also present a wide variety of electric axis orientations, regarding the basal value, accompanied by morphological changes more evident in the RG. The incidence of cardiac lesions histologically detected in the infected group was significantly higher than in C (p < 0.01). It can be concluded that reinfection, neither produce the onset of acute initial disorders nor modify the type or degree of cardiac lesion histologically observed. The ajmaline test showed ECG alterations compatible with a mild myocardial damage in infected rats, whereas a major compromise was assessed in the reinfected group. |
publishDate |
1990 |
dc.date.none.fl_str_mv |
1990-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28743 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28743 |
dc.language.iso.fl_str_mv |
spa |
language |
spa |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28743/30596 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 No. 4 (1990); 260-268 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 Núm. 4 (1990); 260-268 Revista do Instituto de Medicina Tropical de São Paulo; v. 32 n. 4 (1990); 260-268 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951638355935232 |