Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods

Detalhes bibliográficos
Autor(a) principal: Figuerêdo-Silva, José
Data de Publicação: 1991
Outros Autores: Kaneda, Yoshimasa, Tachibana, Hiroshi, Furushima, Rieko, Tateno, Seiki, Correia-Lima, Fernando Gomes, Bento, Dalva Neves da Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28818
Resumo: A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hémoculture, indirect immunofluorescence (IFA), enzyme-linked immunosorbent assay (ELISA), and competitive enzyme-linked immunosorbent assay (C-ELISA) using the monoclonal antibody TCF87 against to a 25kd T. cruzi antigen. Seropositivity was 14.3% by the IFA test, 14.7% by ELISA, and 13.2% by C-ELISA. The C-ELISA using the TCF87 monoclonal antibody seems to be applicable in serodiagnosis of Chagas' disease.
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spelling Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods Pesquisa epidemiologica da infecção por Trypanosoma cruzi no nordeste brasileiro utilizando-se diferentes métodos diagnósticos Trypanosoma cruziEpidemiological surveySerodiagnosisIndirect immunofluorescenceEnzyme-linked immunosorbent assayCompetitive enzyme-linked immunosorbent assay A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hémoculture, indirect immunofluorescence (IFA), enzyme-linked immunosorbent assay (ELISA), and competitive enzyme-linked immunosorbent assay (C-ELISA) using the monoclonal antibody TCF87 against to a 25kd T. cruzi antigen. Seropositivity was 14.3% by the IFA test, 14.7% by ELISA, and 13.2% by C-ELISA. The C-ELISA using the TCF87 monoclonal antibody seems to be applicable in serodiagnosis of Chagas' disease. A prevalência da infecção pelo Trypanosoma cruzi foi pesquisada em Oitis, uma pequena comunidade do Estado do Piauí. Duzentos e sessenta e cinco indivíduos foram investigados pelos seguintes métodos: pesquisa direta, hemocultura, imunofluorescência indireta (IFA), teste imunoabsorvente ligado a enzima (ELISA), e ELISA de competição (C-ELISA) com a utilização do anticorpo monoclonal TCF87 contra um antígeno do T. cruzi com 25kd. A IFA foi positiva em 14,3% dos indivíduos, ELISA em 14,7% e C-ELISA em 13,2%. Este último teste mostrou-se aplicável no diagnóstico sorológico da Doença de Chagas. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1991-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28818Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 3 (1991); 193-198 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 3 (1991); 193-198 Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 3 (1991); 193-198 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28818/30671Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessFiguerêdo-Silva, JoséKaneda, YoshimasaTachibana, HiroshiFurushima, RiekoTateno, SeikiCorreia-Lima, Fernando GomesBento, Dalva Neves da Costa2012-07-02T01:30:23Zoai:revistas.usp.br:article/28818Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:34.878324Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods
Pesquisa epidemiologica da infecção por Trypanosoma cruzi no nordeste brasileiro utilizando-se diferentes métodos diagnósticos
title Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods
spellingShingle Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods
Figuerêdo-Silva, José
Trypanosoma cruzi
Epidemiological survey
Serodiagnosis
Indirect immunofluorescence
Enzyme-linked immunosorbent assay
Competitive enzyme-linked immunosorbent assay
title_short Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods
title_full Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods
title_fullStr Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods
title_full_unstemmed Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods
title_sort Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods
author Figuerêdo-Silva, José
author_facet Figuerêdo-Silva, José
Kaneda, Yoshimasa
Tachibana, Hiroshi
Furushima, Rieko
Tateno, Seiki
Correia-Lima, Fernando Gomes
Bento, Dalva Neves da Costa
author_role author
author2 Kaneda, Yoshimasa
Tachibana, Hiroshi
Furushima, Rieko
Tateno, Seiki
Correia-Lima, Fernando Gomes
Bento, Dalva Neves da Costa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Figuerêdo-Silva, José
Kaneda, Yoshimasa
Tachibana, Hiroshi
Furushima, Rieko
Tateno, Seiki
Correia-Lima, Fernando Gomes
Bento, Dalva Neves da Costa
dc.subject.por.fl_str_mv Trypanosoma cruzi
Epidemiological survey
Serodiagnosis
Indirect immunofluorescence
Enzyme-linked immunosorbent assay
Competitive enzyme-linked immunosorbent assay
topic Trypanosoma cruzi
Epidemiological survey
Serodiagnosis
Indirect immunofluorescence
Enzyme-linked immunosorbent assay
Competitive enzyme-linked immunosorbent assay
description A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hémoculture, indirect immunofluorescence (IFA), enzyme-linked immunosorbent assay (ELISA), and competitive enzyme-linked immunosorbent assay (C-ELISA) using the monoclonal antibody TCF87 against to a 25kd T. cruzi antigen. Seropositivity was 14.3% by the IFA test, 14.7% by ELISA, and 13.2% by C-ELISA. The C-ELISA using the TCF87 monoclonal antibody seems to be applicable in serodiagnosis of Chagas' disease.
publishDate 1991
dc.date.none.fl_str_mv 1991-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28818
url https://www.revistas.usp.br/rimtsp/article/view/28818
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28818/30671
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 3 (1991); 193-198
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 3 (1991); 193-198
Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 3 (1991); 193-198
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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