A simple PCR-based procedure for plaque diagnosis

Detalhes bibliográficos
Autor(a) principal: Leal, Nilma Cintra
Data de Publicação: 1996
Outros Autores: Abath, Frederico Guilherme Coutinho, Alves, Luis Carlos, Almeida, Alzira Maria Paiva de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/29403
Resumo: Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals.
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spelling A simple PCR-based procedure for plaque diagnosis Um método simples para o diagnóstico de peste por PCR PlagueYersinia pestisRodentsPCRDiagnosis Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals. Triturados de baços de animais infectados experimentalmente com Y. pestis, suspensos em salina foram fervidos e os sobrenadantes usados diretamente para amplificação do PCR sem prévia extração do DNA. O limiar de detecção poda ser aumentado por uma segunda etapa de amplificação (Nested-PCR). Não houve amplificação a partir das amostras dos animais não infectados usados como controle. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1996-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29403Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 No. 5 (1996); 371-374 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 Núm. 5 (1996); 371-374 Revista do Instituto de Medicina Tropical de São Paulo; v. 38 n. 5 (1996); 371-374 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29403/31263Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessLeal, Nilma CintraAbath, Frederico Guilherme CoutinhoAlves, Luis CarlosAlmeida, Alzira Maria Paiva de2012-07-02T01:44:20Zoai:revistas.usp.br:article/29403Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:09.105221Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv A simple PCR-based procedure for plaque diagnosis
Um método simples para o diagnóstico de peste por PCR
title A simple PCR-based procedure for plaque diagnosis
spellingShingle A simple PCR-based procedure for plaque diagnosis
Leal, Nilma Cintra
Plague
Yersinia pestis
Rodents
PCR
Diagnosis
title_short A simple PCR-based procedure for plaque diagnosis
title_full A simple PCR-based procedure for plaque diagnosis
title_fullStr A simple PCR-based procedure for plaque diagnosis
title_full_unstemmed A simple PCR-based procedure for plaque diagnosis
title_sort A simple PCR-based procedure for plaque diagnosis
author Leal, Nilma Cintra
author_facet Leal, Nilma Cintra
Abath, Frederico Guilherme Coutinho
Alves, Luis Carlos
Almeida, Alzira Maria Paiva de
author_role author
author2 Abath, Frederico Guilherme Coutinho
Alves, Luis Carlos
Almeida, Alzira Maria Paiva de
author2_role author
author
author
dc.contributor.author.fl_str_mv Leal, Nilma Cintra
Abath, Frederico Guilherme Coutinho
Alves, Luis Carlos
Almeida, Alzira Maria Paiva de
dc.subject.por.fl_str_mv Plague
Yersinia pestis
Rodents
PCR
Diagnosis
topic Plague
Yersinia pestis
Rodents
PCR
Diagnosis
description Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals.
publishDate 1996
dc.date.none.fl_str_mv 1996-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29403
url https://www.revistas.usp.br/rimtsp/article/view/29403
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29403/31263
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 No. 5 (1996); 371-374
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 Núm. 5 (1996); 371-374
Revista do Instituto de Medicina Tropical de São Paulo; v. 38 n. 5 (1996); 371-374
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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