Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs

Detalhes bibliográficos
Autor(a) principal: Kamegasawa, Amélia
Data de Publicação: 1992
Outros Autores: Rezkallah Iwasso, Maria Tereza, Viero, Rosa, Franco, Marcello
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28927
Resumo: The objective of the present study was to develop an efficient and reproducible protocol of immunization of guinea pigs with P. brasiliensis antigens as an animal model for future studies of protective immunity mechanisms. We tested three different antigens (particulate, soluble and combined) and six protocols in the presence and absence of Freund's complete adjuvant and with different numbers of immunizing doses and variable lenght of time between the last immunizing dose and challenge. The efficacy of the immunizing protocol was evaluated by measuring the humoral and cellular anti-P. brasiliensis immune response of the animals, using immuno-diffusion, skin test and macrophage migration inhibition test. It was observed that: 1. Three immunizing doses of the antigens induced a more marked response than two doses; 2. The highest immune response was obtained with the use of Freund's complete adjuvant; 3. Animals challenged a long time (week 6) after the last immunizing dose showed good anti-P. brasiliensis immune response; 4. The particulate antigen induced the lowest immune response. The soluble and the combined antigens were equally efficient in raising good humoral and cellular anti-P. brasiliensis immune response
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spelling Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs Avaliação de diferentes protocolos de imunização em cobaias utilizando antígenos de P. brasiliensis Paracoccidioides brasiliensis antigensGuinea pigsImmunization protocols The objective of the present study was to develop an efficient and reproducible protocol of immunization of guinea pigs with P. brasiliensis antigens as an animal model for future studies of protective immunity mechanisms. We tested three different antigens (particulate, soluble and combined) and six protocols in the presence and absence of Freund's complete adjuvant and with different numbers of immunizing doses and variable lenght of time between the last immunizing dose and challenge. The efficacy of the immunizing protocol was evaluated by measuring the humoral and cellular anti-P. brasiliensis immune response of the animals, using immuno-diffusion, skin test and macrophage migration inhibition test. It was observed that: 1. Three immunizing doses of the antigens induced a more marked response than two doses; 2. The highest immune response was obtained with the use of Freund's complete adjuvant; 3. Animals challenged a long time (week 6) after the last immunizing dose showed good anti-P. brasiliensis immune response; 4. The particulate antigen induced the lowest immune response. The soluble and the combined antigens were equally efficient in raising good humoral and cellular anti-P. brasiliensis immune response O objetivo deste trabalho foi desenvolver protocolo eficiente e reprodutível de imunização em cobaias com antígenos de P. brasiliensis, visando a obtenção de modelo experimental para futuros estudos de mecanismos de proteção imunológica. Testaram-se três diferentes antígenos (particulado, solúvel e composto) e seis protocolos nos quais foram avaliadas as influências dos seguintes fatores: presença ou ausência de adjuvante completo de Freund, número de doses imunizantes e intervalo de tempo entre a última dose imunizante e o desafio. A eficiência do protocolo de imunização foi estudada pela avaliação da resposta imune celular e humoral anti-P. brasiliensis, utilizando teste cutâneo e teste de inibição da migração do macrófago, e imunodifusão, respectivamente. Observou-se que: 1. Três doses imunizantes de antígeno induziram melhor resposta do que duas doses; 2. Maior resposta imune foi conseguida com a utilização de adjuvante completo de Freund; 3. Animais desafiados depois de longo tempo (6 semanas) da última dose imunizante mostraram melhor resposta imune anti-P. brasiliensis; 4. Os antígenos solúvel e composto foram igualmente eficientes induzindo maior resposta imune humoral e celular anti-P. brasiliensis enquanto que o antígeno particulado provocou menor reatividade Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1992-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28927Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 3 (1992); 243-249 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 3 (1992); 243-249 Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 3 (1992); 243-249 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28927/30784Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessKamegasawa, AméliaRezkallah Iwasso, Maria TerezaViero, RosaFranco, Marcello2012-07-02T01:33:03Zoai:revistas.usp.br:article/28927Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:41.533764Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs
Avaliação de diferentes protocolos de imunização em cobaias utilizando antígenos de P. brasiliensis
title Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs
spellingShingle Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs
Kamegasawa, Amélia
Paracoccidioides brasiliensis antigens
Guinea pigs
Immunization protocols
title_short Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs
title_full Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs
title_fullStr Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs
title_full_unstemmed Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs
title_sort Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs
author Kamegasawa, Amélia
author_facet Kamegasawa, Amélia
Rezkallah Iwasso, Maria Tereza
Viero, Rosa
Franco, Marcello
author_role author
author2 Rezkallah Iwasso, Maria Tereza
Viero, Rosa
Franco, Marcello
author2_role author
author
author
dc.contributor.author.fl_str_mv Kamegasawa, Amélia
Rezkallah Iwasso, Maria Tereza
Viero, Rosa
Franco, Marcello
dc.subject.por.fl_str_mv Paracoccidioides brasiliensis antigens
Guinea pigs
Immunization protocols
topic Paracoccidioides brasiliensis antigens
Guinea pigs
Immunization protocols
description The objective of the present study was to develop an efficient and reproducible protocol of immunization of guinea pigs with P. brasiliensis antigens as an animal model for future studies of protective immunity mechanisms. We tested three different antigens (particulate, soluble and combined) and six protocols in the presence and absence of Freund's complete adjuvant and with different numbers of immunizing doses and variable lenght of time between the last immunizing dose and challenge. The efficacy of the immunizing protocol was evaluated by measuring the humoral and cellular anti-P. brasiliensis immune response of the animals, using immuno-diffusion, skin test and macrophage migration inhibition test. It was observed that: 1. Three immunizing doses of the antigens induced a more marked response than two doses; 2. The highest immune response was obtained with the use of Freund's complete adjuvant; 3. Animals challenged a long time (week 6) after the last immunizing dose showed good anti-P. brasiliensis immune response; 4. The particulate antigen induced the lowest immune response. The soluble and the combined antigens were equally efficient in raising good humoral and cellular anti-P. brasiliensis immune response
publishDate 1992
dc.date.none.fl_str_mv 1992-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28927
url https://www.revistas.usp.br/rimtsp/article/view/28927
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28927/30784
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 3 (1992); 243-249
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 3 (1992); 243-249
Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 3 (1992); 243-249
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
_version_ 1798951639445405696

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