Coaglutination test (COA) for Cryptococcus neoformans circulating antigen detection in cerebral spinal fluid (CSF)
Autor(a) principal: | |
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Data de Publicação: | 1990 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28778 |
Resumo: | We tested 82 CSF of 24 renal transplanted patients (RT) with cerebral cryptococcosis, 8 CSF of asymptomatic RT patients, 43 CSF of proven cryptococcosis cases (positive control) and 35 CSF of patients with other diseases (histoplasmosis, paracoccidioidomycosis and bacterial infections) as negative control. The RT CSF were cultured in Sabouraud agar slant added with sunflower seeds and both control and RT CSF were qualitatively examined by cryptococcosis latex test (Crypto-LA test). The COA test was developed both qualitatively and quantitatively. The highest titre encountered was 1:2048. No false reactions appeared among the controls. The diagnostic value demonstrated by Galen and Gambino's method was: sensitivity - 92.1%; specificity - 92.6% and efficiency - 92.3%. Besides that, the COA proved to be quick, exact and cheap, but it depends on CSF and sera pre-treatment, in order to avoid autoagglutination and increase its sensibility. |
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Revista do Instituto de Medicina Tropical de São Paulo |
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Coaglutination test (COA) for Cryptococcus neoformans circulating antigen detection in cerebral spinal fluid (CSF) Pesquisa do antígeno circulante de Cryptococcus neoformans em líquido cefalorraqueano pelo teste de coaglutinação Coagglutination TestCryptococcus neoformansAntigen detection We tested 82 CSF of 24 renal transplanted patients (RT) with cerebral cryptococcosis, 8 CSF of asymptomatic RT patients, 43 CSF of proven cryptococcosis cases (positive control) and 35 CSF of patients with other diseases (histoplasmosis, paracoccidioidomycosis and bacterial infections) as negative control. The RT CSF were cultured in Sabouraud agar slant added with sunflower seeds and both control and RT CSF were qualitatively examined by cryptococcosis latex test (Crypto-LA test). The COA test was developed both qualitatively and quantitatively. The highest titre encountered was 1:2048. No false reactions appeared among the controls. The diagnostic value demonstrated by Galen and Gambino's method was: sensitivity - 92.1%; specificity - 92.6% and efficiency - 92.3%. Besides that, the COA proved to be quick, exact and cheap, but it depends on CSF and sera pre-treatment, in order to avoid autoagglutination and increase its sensibility. Foram utilizadas 82 LCR de transplantados renais (24 pacientes), 43 LCR de pacientes com críptococose comprovada (controles positivos), 35 LCR de pacientes com outras doenças (histoplasmose, paracoccidioidomicose e infecções bacterianas) como controles negativos. Os primeiros foram cultivados em ágar Sabouraud com sementes de girassol e juntamente com os demais examinado pelo teste de látex para pesquisa de antígeno circulante de C. neoformans, qualitativamente. O teste de Coaglutinação foi realizado qualitativamente e quantitativamente, encontrando-se títulos até a diluição 1:2048. Não foram detectadas reações falso-positivas ou falso-negativas entre os controles. Como prova de valor diagnóstico demonstrou: sensibilidade - 92,1%; especificidade - 92,6% e eficiência - 92,3%. Provou também ser um teste rápido, exato e econômico, embora sua escolha dependa do pré-tratamento de LCR (80ºC por 3 a 5 minutos) e soros (diluição ou álcali-precipitação) para evitar autoaglutinação e aumentar a sensibilidade da reação. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1990-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28778Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 No. 6 (1990); 456-460 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 Núm. 6 (1990); 456-460 Revista do Instituto de Medicina Tropical de São Paulo; v. 32 n. 6 (1990); 456-460 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/28778/30631Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCano, Maria Isabel NogueiraLivramento, José Antonio2012-07-02T01:28:58Zoai:revistas.usp.br:article/28778Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:32.312906Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Coaglutination test (COA) for Cryptococcus neoformans circulating antigen detection in cerebral spinal fluid (CSF) Pesquisa do antígeno circulante de Cryptococcus neoformans em líquido cefalorraqueano pelo teste de coaglutinação |
title |
Coaglutination test (COA) for Cryptococcus neoformans circulating antigen detection in cerebral spinal fluid (CSF) |
spellingShingle |
Coaglutination test (COA) for Cryptococcus neoformans circulating antigen detection in cerebral spinal fluid (CSF) Cano, Maria Isabel Nogueira Coagglutination Test Cryptococcus neoformans Antigen detection |
title_short |
Coaglutination test (COA) for Cryptococcus neoformans circulating antigen detection in cerebral spinal fluid (CSF) |
title_full |
Coaglutination test (COA) for Cryptococcus neoformans circulating antigen detection in cerebral spinal fluid (CSF) |
title_fullStr |
Coaglutination test (COA) for Cryptococcus neoformans circulating antigen detection in cerebral spinal fluid (CSF) |
title_full_unstemmed |
Coaglutination test (COA) for Cryptococcus neoformans circulating antigen detection in cerebral spinal fluid (CSF) |
title_sort |
Coaglutination test (COA) for Cryptococcus neoformans circulating antigen detection in cerebral spinal fluid (CSF) |
author |
Cano, Maria Isabel Nogueira |
author_facet |
Cano, Maria Isabel Nogueira Livramento, José Antonio |
author_role |
author |
author2 |
Livramento, José Antonio |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Cano, Maria Isabel Nogueira Livramento, José Antonio |
dc.subject.por.fl_str_mv |
Coagglutination Test Cryptococcus neoformans Antigen detection |
topic |
Coagglutination Test Cryptococcus neoformans Antigen detection |
description |
We tested 82 CSF of 24 renal transplanted patients (RT) with cerebral cryptococcosis, 8 CSF of asymptomatic RT patients, 43 CSF of proven cryptococcosis cases (positive control) and 35 CSF of patients with other diseases (histoplasmosis, paracoccidioidomycosis and bacterial infections) as negative control. The RT CSF were cultured in Sabouraud agar slant added with sunflower seeds and both control and RT CSF were qualitatively examined by cryptococcosis latex test (Crypto-LA test). The COA test was developed both qualitatively and quantitatively. The highest titre encountered was 1:2048. No false reactions appeared among the controls. The diagnostic value demonstrated by Galen and Gambino's method was: sensitivity - 92.1%; specificity - 92.6% and efficiency - 92.3%. Besides that, the COA proved to be quick, exact and cheap, but it depends on CSF and sera pre-treatment, in order to avoid autoagglutination and increase its sensibility. |
publishDate |
1990 |
dc.date.none.fl_str_mv |
1990-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28778 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28778 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28778/30631 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 No. 6 (1990); 456-460 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 Núm. 6 (1990); 456-460 Revista do Instituto de Medicina Tropical de São Paulo; v. 32 n. 6 (1990); 456-460 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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