Fractionation of Crotalus durissus terrificus of chromatography technique by molecular exclusion
Autor(a) principal: | |
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Data de Publicação: | 1985 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/87369 |
Resumo: | By means of chromatography technique by molecular exclusion using Sephadex G-75 were studied different fractions of venom Crotalus durissus terrificus, one the most common poisonous snakes in Brazil. It were obtained four peaks related to fractions 32, 60, 86 and 103, which molecular weight varied from 4.000 to 150.000. The fractions of all gel filtration diagram were screened through immunoelectrophoresis, in order to verify its charges and migration speed. The precipitation lines were compared with 11 lines obtained through immunoelectrophoresis of total venom against anti-Crotalus durissus terrificus serum. It was observed that fractions of same peak presented peculiar characteristics except fraction 54 (corresponding to the ascending of peak II), which showed significant differences when compared with fraction 60. After screening, fractions of each peak were chosen in order to identify means of double radial diffusion test and the letality was compared with 0,0625 mg/ml concentration of total venom, which corresponds to DL50 in mice by SPEARM & KÄRBER method. Fractions 32, 86 and 103 corresponding to peaks I, III and IV respectively, presented null or negligibe lethality, and fraction II was the most toxic. |
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Fractionation of Crotalus durissus terrificus of chromatography technique by molecular exclusion Fracionamento do veneno Crotalus durissus terrificus por cromatografia de exclusão molecular By means of chromatography technique by molecular exclusion using Sephadex G-75 were studied different fractions of venom Crotalus durissus terrificus, one the most common poisonous snakes in Brazil. It were obtained four peaks related to fractions 32, 60, 86 and 103, which molecular weight varied from 4.000 to 150.000. The fractions of all gel filtration diagram were screened through immunoelectrophoresis, in order to verify its charges and migration speed. The precipitation lines were compared with 11 lines obtained through immunoelectrophoresis of total venom against anti-Crotalus durissus terrificus serum. It was observed that fractions of same peak presented peculiar characteristics except fraction 54 (corresponding to the ascending of peak II), which showed significant differences when compared with fraction 60. After screening, fractions of each peak were chosen in order to identify means of double radial diffusion test and the letality was compared with 0,0625 mg/ml concentration of total venom, which corresponds to DL50 in mice by SPEARM & KÄRBER method. Fractions 32, 86 and 103 corresponding to peaks I, III and IV respectively, presented null or negligibe lethality, and fraction II was the most toxic. Através da técnica de cromatografia de exclusão molecular utilizando Sephadex G-75 foram estudadas as diferentes frações do veneno de Crotalus durissus terrificus, uma das serpentes peçonhentas mais comuns no Brasil. Foram obtidos 4 picos (correspondentes às frações 32, 60, 86 e 103) com peso molecular variando de 4.000 a 150.000. As frações de todo o diagrama de gel filtração foram triadas através de reação de imunoeletroforese a fim de se verificar suas cargas e velocidade de migração. As linhas de precipitação encontradas foram comparadas às 11 linhas apresentadas pela reação de imunoeletroforese do veneno total contra o soro anti-crotálico. Constatou-se que as frações de um mesmo pico apresentavam características próprias com exceção da fração 54 (subida do pico II) que mostrou diferenças significativas em relação à fração 60. Após a triagem foram escolhidas as frações de cada pico onde as linhas de precipitação foram mais nítidas e intensas, para estudo de identidade através da reação de difusão radial dupla e letalidade comparada a concentração de 0,0625 mg/ml do veneno total que corresponde a DL50 em camundongo pelo método de SPEARM & KÄRBER. As frações 32, 86 e 103 correspondentes respectivamente aos picos I, III e IV apresentaram letalidade nula ou negligenciada e a fração II foi a mais tóxica. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1985-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/87369Revista do Instituto de Medicina Tropical de São Paulo; Vol. 27 No. 3 (1985); 115-122Revista do Instituto de Medicina Tropical de São Paulo; Vol. 27 Núm. 3 (1985); 115-122Revista do Instituto de Medicina Tropical de São Paulo; v. 27 n. 3 (1985); 115-1221678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/87369/90328Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessTakeda, Augusta KiyomiBarbosa, Sônia França CorreiaCosta, Lucinda Maria daAdelino, Maria das Graças Fernandes2014-11-07T16:45:36Zoai:revistas.usp.br:article/87369Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:23.417501Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Fractionation of Crotalus durissus terrificus of chromatography technique by molecular exclusion Fracionamento do veneno Crotalus durissus terrificus por cromatografia de exclusão molecular |
title |
Fractionation of Crotalus durissus terrificus of chromatography technique by molecular exclusion |
spellingShingle |
Fractionation of Crotalus durissus terrificus of chromatography technique by molecular exclusion Takeda, Augusta Kiyomi |
title_short |
Fractionation of Crotalus durissus terrificus of chromatography technique by molecular exclusion |
title_full |
Fractionation of Crotalus durissus terrificus of chromatography technique by molecular exclusion |
title_fullStr |
Fractionation of Crotalus durissus terrificus of chromatography technique by molecular exclusion |
title_full_unstemmed |
Fractionation of Crotalus durissus terrificus of chromatography technique by molecular exclusion |
title_sort |
Fractionation of Crotalus durissus terrificus of chromatography technique by molecular exclusion |
author |
Takeda, Augusta Kiyomi |
author_facet |
Takeda, Augusta Kiyomi Barbosa, Sônia França Correia Costa, Lucinda Maria da Adelino, Maria das Graças Fernandes |
author_role |
author |
author2 |
Barbosa, Sônia França Correia Costa, Lucinda Maria da Adelino, Maria das Graças Fernandes |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Takeda, Augusta Kiyomi Barbosa, Sônia França Correia Costa, Lucinda Maria da Adelino, Maria das Graças Fernandes |
description |
By means of chromatography technique by molecular exclusion using Sephadex G-75 were studied different fractions of venom Crotalus durissus terrificus, one the most common poisonous snakes in Brazil. It were obtained four peaks related to fractions 32, 60, 86 and 103, which molecular weight varied from 4.000 to 150.000. The fractions of all gel filtration diagram were screened through immunoelectrophoresis, in order to verify its charges and migration speed. The precipitation lines were compared with 11 lines obtained through immunoelectrophoresis of total venom against anti-Crotalus durissus terrificus serum. It was observed that fractions of same peak presented peculiar characteristics except fraction 54 (corresponding to the ascending of peak II), which showed significant differences when compared with fraction 60. After screening, fractions of each peak were chosen in order to identify means of double radial diffusion test and the letality was compared with 0,0625 mg/ml concentration of total venom, which corresponds to DL50 in mice by SPEARM & KÄRBER method. Fractions 32, 86 and 103 corresponding to peaks I, III and IV respectively, presented null or negligibe lethality, and fraction II was the most toxic. |
publishDate |
1985 |
dc.date.none.fl_str_mv |
1985-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/87369 |
url |
https://www.revistas.usp.br/rimtsp/article/view/87369 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/87369/90328 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 27 No. 3 (1985); 115-122 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 27 Núm. 3 (1985); 115-122 Revista do Instituto de Medicina Tropical de São Paulo; v. 27 n. 3 (1985); 115-122 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951650081112064 |