Epidemiological and molecular surveillance of norovirus in the Brazilian Amazon: description of recombinant genotypes and improvement of evolutionary analysis
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/224335 |
Resumo: | Noroviruses are highly infectious, genetically diverse viruses. Global outbreaks occur frequently, making molecular surveillance important for infection monitoring. This cross-sectional descriptive study aimed to monitor cases of norovirus gastroenteritis in the Brazilian Amazon. Fecal samples were tested by immunoenzymatic assay, RT-PCR and genetic sequencing for the ORF1/ORF2 and protease regions. Bayesian inference with a molecular clock was employed to construct the phylogeny. The norovirus prevalence was 25.8%, with a higher positivity rate among children aged 0-24 months. Genogroup GII accounted for 98.1% of the sequenced samples, while GI accounted for 1.9% of them. The GII.P16/GII.4 genotype was the most prevalent, with an evolution rate of 2.87x10−3 and TMRCA estimated in 2012. This study demonstrates that norovirus is a primary causative agent of gastroenteritis and provides data on viral genetic diversity that may facilitate infection surveillance and vaccine development. |
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Revista do Instituto de Medicina Tropical de São Paulo |
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Epidemiological and molecular surveillance of norovirus in the Brazilian Amazon: description of recombinant genotypes and improvement of evolutionary analysisNorovirusDiarrheaGastroenteritisAmazon RegionMolecular evolutionNoroviruses are highly infectious, genetically diverse viruses. Global outbreaks occur frequently, making molecular surveillance important for infection monitoring. This cross-sectional descriptive study aimed to monitor cases of norovirus gastroenteritis in the Brazilian Amazon. Fecal samples were tested by immunoenzymatic assay, RT-PCR and genetic sequencing for the ORF1/ORF2 and protease regions. Bayesian inference with a molecular clock was employed to construct the phylogeny. The norovirus prevalence was 25.8%, with a higher positivity rate among children aged 0-24 months. Genogroup GII accounted for 98.1% of the sequenced samples, while GI accounted for 1.9% of them. The GII.P16/GII.4 genotype was the most prevalent, with an evolution rate of 2.87x10−3 and TMRCA estimated in 2012. This study demonstrates that norovirus is a primary causative agent of gastroenteritis and provides data on viral genetic diversity that may facilitate infection surveillance and vaccine development.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2024-04-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/22433510.1590/Revista do Instituto de Medicina Tropical de São Paulo; Vol. 66 (2024); e22Revista do Instituto de Medicina Tropical de São Paulo; v. 66 (2024); e22Revista do Instituto de Medicina Tropical de São Paulo; Vol. 66 (2024); e221678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/224335/203941Copyright (c) 2024 Jonaia Novaes da Costa, Jones Anderson Monteiro Siqueira, Dielle Monteiro Teixeira, Patrícia dos Santos Lobo, Sylvia de Fátima dos Santos Guerra, Isadora Monteiro Souza, Bruna Trindade Moreira Cardoso, Luana Silva Soares Farias, Hugo Reis Resque, Yvone Benchimol Gabbay, Luciana Damascena da Silvahttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccess Costa, Jonaia Novaes daSiqueira, Jones Anderson MonteiroTeixeira, Dielle MonteiroLobo, Patrícia dos SantosGuerra, Sylvia de Fátima dos SantosSouza, Isadora MonteiroCardoso, Bruna Trindade MoreiraFarias, Luana Silva SoaresResque, Hugo ReisGabbay, Yvone BenchimolSilva, Luciana Damascena da2024-05-06T14:04:52Zoai:revistas.usp.br:article/224335Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2024-05-06T14:04:52Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)false |
dc.title.none.fl_str_mv |
Epidemiological and molecular surveillance of norovirus in the Brazilian Amazon: description of recombinant genotypes and improvement of evolutionary analysis |
title |
Epidemiological and molecular surveillance of norovirus in the Brazilian Amazon: description of recombinant genotypes and improvement of evolutionary analysis |
spellingShingle |
Epidemiological and molecular surveillance of norovirus in the Brazilian Amazon: description of recombinant genotypes and improvement of evolutionary analysis Costa, Jonaia Novaes da Norovirus Diarrhea Gastroenteritis Amazon Region Molecular evolution |
title_short |
Epidemiological and molecular surveillance of norovirus in the Brazilian Amazon: description of recombinant genotypes and improvement of evolutionary analysis |
title_full |
Epidemiological and molecular surveillance of norovirus in the Brazilian Amazon: description of recombinant genotypes and improvement of evolutionary analysis |
title_fullStr |
Epidemiological and molecular surveillance of norovirus in the Brazilian Amazon: description of recombinant genotypes and improvement of evolutionary analysis |
title_full_unstemmed |
Epidemiological and molecular surveillance of norovirus in the Brazilian Amazon: description of recombinant genotypes and improvement of evolutionary analysis |
title_sort |
Epidemiological and molecular surveillance of norovirus in the Brazilian Amazon: description of recombinant genotypes and improvement of evolutionary analysis |
author |
Costa, Jonaia Novaes da |
author_facet |
Costa, Jonaia Novaes da Siqueira, Jones Anderson Monteiro Teixeira, Dielle Monteiro Lobo, Patrícia dos Santos Guerra, Sylvia de Fátima dos Santos Souza, Isadora Monteiro Cardoso, Bruna Trindade Moreira Farias, Luana Silva Soares Resque, Hugo Reis Gabbay, Yvone Benchimol Silva, Luciana Damascena da |
author_role |
author |
author2 |
Siqueira, Jones Anderson Monteiro Teixeira, Dielle Monteiro Lobo, Patrícia dos Santos Guerra, Sylvia de Fátima dos Santos Souza, Isadora Monteiro Cardoso, Bruna Trindade Moreira Farias, Luana Silva Soares Resque, Hugo Reis Gabbay, Yvone Benchimol Silva, Luciana Damascena da |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Jonaia Novaes da Siqueira, Jones Anderson Monteiro Teixeira, Dielle Monteiro Lobo, Patrícia dos Santos Guerra, Sylvia de Fátima dos Santos Souza, Isadora Monteiro Cardoso, Bruna Trindade Moreira Farias, Luana Silva Soares Resque, Hugo Reis Gabbay, Yvone Benchimol Silva, Luciana Damascena da |
dc.subject.por.fl_str_mv |
Norovirus Diarrhea Gastroenteritis Amazon Region Molecular evolution |
topic |
Norovirus Diarrhea Gastroenteritis Amazon Region Molecular evolution |
description |
Noroviruses are highly infectious, genetically diverse viruses. Global outbreaks occur frequently, making molecular surveillance important for infection monitoring. This cross-sectional descriptive study aimed to monitor cases of norovirus gastroenteritis in the Brazilian Amazon. Fecal samples were tested by immunoenzymatic assay, RT-PCR and genetic sequencing for the ORF1/ORF2 and protease regions. Bayesian inference with a molecular clock was employed to construct the phylogeny. The norovirus prevalence was 25.8%, with a higher positivity rate among children aged 0-24 months. Genogroup GII accounted for 98.1% of the sequenced samples, while GI accounted for 1.9% of them. The GII.P16/GII.4 genotype was the most prevalent, with an evolution rate of 2.87x10−3 and TMRCA estimated in 2012. This study demonstrates that norovirus is a primary causative agent of gastroenteritis and provides data on viral genetic diversity that may facilitate infection surveillance and vaccine development. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-04-25 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/224335 10.1590/ |
url |
https://www.revistas.usp.br/rimtsp/article/view/224335 |
identifier_str_mv |
10.1590/ |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/224335/203941 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 66 (2024); e22 Revista do Instituto de Medicina Tropical de São Paulo; v. 66 (2024); e22 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 66 (2024); e22 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951636666679296 |