Influenza virus and proteolytic bacteria co-infection in respiratory tract from individuals presenting respiratory manifestations

Detalhes bibliográficos
Autor(a) principal: Mancini, Dalva Assunção Portari
Data de Publicação: 2008
Outros Autores: Alves, Rosely C. Barbosa, Mendonça, Rita Maria Zucatelli, Bellei, Nancy J., Carraro, Emerson, Machado, Antonia M.O., Pinto, José Ricardo, Mancini Filho, Jorge
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31146
Resumo: A role for proteolytic bacteria in the exacerbation of influenza virus has been shown in natural hosts such as pigs and humans. Four hundred seven samples were collected from the respiratory tract of individuals presenting clinical manifestations, during influenza season (2003-2005) in São Paulo City. The aim of this study was to evaluate the incidence of determined bacteria co-infecting virus in human respiratory tract. Tests, such as bacteriological, immunofluorescence (IF), RT/PCR and hemagglutination (HA) were used for bacterial and viral investigation. Thirty seven (9.09%) positive for influenza virus were screened by IF. The RT/PCR confirmed the presence of influenza virus in these samples. Bacterial and agar casein tests demonstrated that 18 (48.64%) individuals were infected with proteolytic bacteria such as Staphylococcus spp., Streptococcus spp. and Pseudomonas spp. Among these samples, 13 (35.13%) were co-infected with influenza A virus. Influenza type B, co-infecting bacteria were found in five (13.51%) samples. In vitro the S. aureus protease increased the influenza HA titer after contact for 30 min at 25 ºC. Results revealed the occurrence of co-infection with proteolytic bacteria and influenza in the evaluated individuals. This finding corroborates that virus versus bacteria synergism could be able to potentiate respiratory infection, increasing damage to hosts.
id IMT-1_25eddf45b8b84192da8b2690376b2fd8
oai_identifier_str oai:revistas.usp.br:article/31146
network_acronym_str IMT-1
network_name_str Revista do Instituto de Medicina Tropical de São Paulo
repository_id_str
spelling Influenza virus and proteolytic bacteria co-infection in respiratory tract from individuals presenting respiratory manifestations Vírus influenza e bactéria proteolítica co-infectantes em trato respiratório de indivíduos com manifestações respiratórias InfluenzaProteolytic bacteriaCo-infectionRespiratory tract A role for proteolytic bacteria in the exacerbation of influenza virus has been shown in natural hosts such as pigs and humans. Four hundred seven samples were collected from the respiratory tract of individuals presenting clinical manifestations, during influenza season (2003-2005) in São Paulo City. The aim of this study was to evaluate the incidence of determined bacteria co-infecting virus in human respiratory tract. Tests, such as bacteriological, immunofluorescence (IF), RT/PCR and hemagglutination (HA) were used for bacterial and viral investigation. Thirty seven (9.09%) positive for influenza virus were screened by IF. The RT/PCR confirmed the presence of influenza virus in these samples. Bacterial and agar casein tests demonstrated that 18 (48.64%) individuals were infected with proteolytic bacteria such as Staphylococcus spp., Streptococcus spp. and Pseudomonas spp. Among these samples, 13 (35.13%) were co-infected with influenza A virus. Influenza type B, co-infecting bacteria were found in five (13.51%) samples. In vitro the S. aureus protease increased the influenza HA titer after contact for 30 min at 25 ºC. Results revealed the occurrence of co-infection with proteolytic bacteria and influenza in the evaluated individuals. This finding corroborates that virus versus bacteria synergism could be able to potentiate respiratory infection, increasing damage to hosts. O papel da bactéria proteolítica na exacerbação do vírus influenza tem sido demonstrado em hospedeiros naturais como porcos e humanos. Foram coletadas 407 amostras do trato respiratório de indivíduos apresentando manifestações clínicas, durante a estação da influenza (2003-2005) na cidade de São Paulo. Este trabalho teve como objetivo avaliar a incidência de determinadas bactérias que junto com vírus co-infectarem o trato respiratório humano. Testes bacteriológicos, e virológicos como imunofluorescência (IF), RT/PCR e hemaglutinação (HA) foram usados nas investigações viral e bacteriana. Pelo teste de IF foram selecionadas trinta e sete (9,09%) amostras positivas para o vírus influenza. A presença do vírus influenza foi confirmada pela técnica de RT/PCR. Pelos testes bacteriológicos e do agar caseina, verificou-se que 18 (48,64%) dos indivíduos foram infectados com bactérias proteolíticas tais como Staphylococcus spp., Streptococcus spp. e Pseudomonas spp. Destas amostras, 13 (35,13%) foram co-infectadas com vírus influenza tipo A, e 5 (13,51%) com influenza tipo B. No experimento in vitro com influenza e S. aureus, detectou-se aumento do título hemaglutinante deste vírus, após contacto de 30 min a 25 ºC. Os resultados obtidos revelaram a ocorrência de co-infecção com bactéria proteolítica e vírus influenza nos indivíduos avaliados. Estes achados corroboram com a investigação do sinergismo, entre bactéria e vírus, que poderia ser capaz de potencializar infecção respiratória, aumentando os riscos aos hospedeiros. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2008-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31146Revista do Instituto de Medicina Tropical de São Paulo; Vol. 50 No. 1 (2008); 41-46 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 50 Núm. 1 (2008); 41-46 Revista do Instituto de Medicina Tropical de São Paulo; v. 50 n. 1 (2008); 41-46 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31146/33030Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessMancini, Dalva Assunção PortariAlves, Rosely C. BarbosaMendonça, Rita Maria ZucatelliBellei, Nancy J.Carraro, EmersonMachado, Antonia M.O.Pinto, José RicardoMancini Filho, Jorge2012-07-07T19:06:57Zoai:revistas.usp.br:article/31146Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:50.403743Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Influenza virus and proteolytic bacteria co-infection in respiratory tract from individuals presenting respiratory manifestations
Vírus influenza e bactéria proteolítica co-infectantes em trato respiratório de indivíduos com manifestações respiratórias
title Influenza virus and proteolytic bacteria co-infection in respiratory tract from individuals presenting respiratory manifestations
spellingShingle Influenza virus and proteolytic bacteria co-infection in respiratory tract from individuals presenting respiratory manifestations
Mancini, Dalva Assunção Portari
Influenza
Proteolytic bacteria
Co-infection
Respiratory tract
title_short Influenza virus and proteolytic bacteria co-infection in respiratory tract from individuals presenting respiratory manifestations
title_full Influenza virus and proteolytic bacteria co-infection in respiratory tract from individuals presenting respiratory manifestations
title_fullStr Influenza virus and proteolytic bacteria co-infection in respiratory tract from individuals presenting respiratory manifestations
title_full_unstemmed Influenza virus and proteolytic bacteria co-infection in respiratory tract from individuals presenting respiratory manifestations
title_sort Influenza virus and proteolytic bacteria co-infection in respiratory tract from individuals presenting respiratory manifestations
author Mancini, Dalva Assunção Portari
author_facet Mancini, Dalva Assunção Portari
Alves, Rosely C. Barbosa
Mendonça, Rita Maria Zucatelli
Bellei, Nancy J.
Carraro, Emerson
Machado, Antonia M.O.
Pinto, José Ricardo
Mancini Filho, Jorge
author_role author
author2 Alves, Rosely C. Barbosa
Mendonça, Rita Maria Zucatelli
Bellei, Nancy J.
Carraro, Emerson
Machado, Antonia M.O.
Pinto, José Ricardo
Mancini Filho, Jorge
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mancini, Dalva Assunção Portari
Alves, Rosely C. Barbosa
Mendonça, Rita Maria Zucatelli
Bellei, Nancy J.
Carraro, Emerson
Machado, Antonia M.O.
Pinto, José Ricardo
Mancini Filho, Jorge
dc.subject.por.fl_str_mv Influenza
Proteolytic bacteria
Co-infection
Respiratory tract
topic Influenza
Proteolytic bacteria
Co-infection
Respiratory tract
description A role for proteolytic bacteria in the exacerbation of influenza virus has been shown in natural hosts such as pigs and humans. Four hundred seven samples were collected from the respiratory tract of individuals presenting clinical manifestations, during influenza season (2003-2005) in São Paulo City. The aim of this study was to evaluate the incidence of determined bacteria co-infecting virus in human respiratory tract. Tests, such as bacteriological, immunofluorescence (IF), RT/PCR and hemagglutination (HA) were used for bacterial and viral investigation. Thirty seven (9.09%) positive for influenza virus were screened by IF. The RT/PCR confirmed the presence of influenza virus in these samples. Bacterial and agar casein tests demonstrated that 18 (48.64%) individuals were infected with proteolytic bacteria such as Staphylococcus spp., Streptococcus spp. and Pseudomonas spp. Among these samples, 13 (35.13%) were co-infected with influenza A virus. Influenza type B, co-infecting bacteria were found in five (13.51%) samples. In vitro the S. aureus protease increased the influenza HA titer after contact for 30 min at 25 ºC. Results revealed the occurrence of co-infection with proteolytic bacteria and influenza in the evaluated individuals. This finding corroborates that virus versus bacteria synergism could be able to potentiate respiratory infection, increasing damage to hosts.
publishDate 2008
dc.date.none.fl_str_mv 2008-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31146
url https://www.revistas.usp.br/rimtsp/article/view/31146
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31146/33030
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 50 No. 1 (2008); 41-46
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 50 Núm. 1 (2008); 41-46
Revista do Instituto de Medicina Tropical de São Paulo; v. 50 n. 1 (2008); 41-46
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
_version_ 1798951646808506368

Registros relacionados