Antifungal susceptibilities of clinical and environmental isolates of Cryptococcus neoformans in goiânia city, Goiás, Brasil

Detalhes bibliográficos
Autor(a) principal: Souza, Lúcia Kioko Hasimoto
Data de Publicação: 2005
Outros Autores: Fernandes, Orionalda de Fátima Lisboa, Kobayashi, Cláudia Castelo Branco Artiaga, Passos, Xisto Sena, Costa, Carolina Rodrigues, Lemos, Janine Aquino, Souza-Júnior, Ary Henrique, Silva, Maria do Rosário Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30934
Resumo: We evaluated the antifungal activities of amphotericin B, fluconazole, itraconazole and voriconazole in 70 Cryptococcus neoformans strains obtained from cerebrospinal fluid from AIDS patients and 40 C. neoformans strains isolated from the environment. Four clinical isolates were identified as C. neoformans var. gattii. The susceptibility test was done using a broth microdilution method according to NCCLS M27-A2. Range minimal inhibitory concentrations (MICs) for C. neoformans clinical isolates were 0.06-1.0 µg/mL for amphotericin B, 0.125-8 µg/mL for fluconazole, 0.03-0.5 µg/mL for itraconazole and 0.03-0.25 µg/mL for voriconazole. C. neoformans environmental isolates showed range MICs 0.015-0.125 µg/mL, 0.25-2.0 µg/mL, 0.007-0.125 µg/mL and 0.03-0.25 µg/mL for amphotericin B, fluconazole, itraconazole and voriconazole respectively. The MICs results obtained from clinical and environmental isolates showed similar pattern of susceptibility and no resistance has been found in our isolates.
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spelling Antifungal susceptibilities of clinical and environmental isolates of Cryptococcus neoformans in goiânia city, Goiás, Brasil Suscetibilidade antifúngica de isolados clínicos e ambientais de Cryptococcus neoformans na cidade de Goiânia, Goiás, Brasil Cryptococcus neoformansAntifungal agentsCerebrospinal fluidEnvironment We evaluated the antifungal activities of amphotericin B, fluconazole, itraconazole and voriconazole in 70 Cryptococcus neoformans strains obtained from cerebrospinal fluid from AIDS patients and 40 C. neoformans strains isolated from the environment. Four clinical isolates were identified as C. neoformans var. gattii. The susceptibility test was done using a broth microdilution method according to NCCLS M27-A2. Range minimal inhibitory concentrations (MICs) for C. neoformans clinical isolates were 0.06-1.0 µg/mL for amphotericin B, 0.125-8 µg/mL for fluconazole, 0.03-0.5 µg/mL for itraconazole and 0.03-0.25 µg/mL for voriconazole. C. neoformans environmental isolates showed range MICs 0.015-0.125 µg/mL, 0.25-2.0 µg/mL, 0.007-0.125 µg/mL and 0.03-0.25 µg/mL for amphotericin B, fluconazole, itraconazole and voriconazole respectively. The MICs results obtained from clinical and environmental isolates showed similar pattern of susceptibility and no resistance has been found in our isolates. A atividade antifúngica de anfotericina B, fluconazol, itraconazol e voriconazol foi avaliada em 70 amostras de Cryptococcus neoformans isoladas de liquido céfalo raquidiano (LCR) de pacientes com AIDS e em 40 amostras de C. neoformans obtidas do meio ambiente. Dentre os isolados 66 foram identificados como C. neoformans var. neoformans e quatro isolados clínicos, como C. neoformans var. gattii. Para a realização dos testes de suscetibilidade foi utilizado o método de microdiluição em meio líquido segundo o NCCLS M27-A2. As concentrações inibitórias mínimas (CIMs) para os isolados clínicos variaram de 0,06-1,0 µg/mL para anfotericina B, 0,125-8 µg/mL para fluconazol, 0,03-0,5 µg/mL para itraconazol e 0,03-0,25 µg/mL para voriconazol, enquanto que para as amostras ambientais de C. neoformans as concentrações inibitórias variaram de 0,015-0,125 µg/mL, 0,25-2,0 µg/mL, 0,007-0,125 µg/mL e 0,03-0,25 µg/mL para anfotericina B, fluconazol, itraconazol e voriconazol, respectivamente. Os resultados das concentrações inibitórias mínimas obtidas para os isolados clínicos e ambientais mostraram semelhança com relação ao perfil de suscetibilidade, não tendo sido encontrados isolados resistentes a nenhum dos antifúngicos, levando-se em consideração a metodologia e critério de interpretação estudados. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2005-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30934Revista do Instituto de Medicina Tropical de São Paulo; Vol. 47 No. 5 (2005); 253-256 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 47 Núm. 5 (2005); 253-256 Revista do Instituto de Medicina Tropical de São Paulo; v. 47 n. 5 (2005); 253-256 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30934/32818Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessSouza, Lúcia Kioko HasimotoFernandes, Orionalda de Fátima LisboaKobayashi, Cláudia Castelo Branco ArtiagaPassos, Xisto SenaCosta, Carolina RodriguesLemos, Janine AquinoSouza-Júnior, Ary HenriqueSilva, Maria do Rosário Rodrigues2012-07-07T18:38:05Zoai:revistas.usp.br:article/30934Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2012-07-07T18:38:05Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)false
dc.title.none.fl_str_mv Antifungal susceptibilities of clinical and environmental isolates of Cryptococcus neoformans in goiânia city, Goiás, Brasil
Suscetibilidade antifúngica de isolados clínicos e ambientais de Cryptococcus neoformans na cidade de Goiânia, Goiás, Brasil
title Antifungal susceptibilities of clinical and environmental isolates of Cryptococcus neoformans in goiânia city, Goiás, Brasil
spellingShingle Antifungal susceptibilities of clinical and environmental isolates of Cryptococcus neoformans in goiânia city, Goiás, Brasil
Souza, Lúcia Kioko Hasimoto
Cryptococcus neoformans
Antifungal agents
Cerebrospinal fluid
Environment
title_short Antifungal susceptibilities of clinical and environmental isolates of Cryptococcus neoformans in goiânia city, Goiás, Brasil
title_full Antifungal susceptibilities of clinical and environmental isolates of Cryptococcus neoformans in goiânia city, Goiás, Brasil
title_fullStr Antifungal susceptibilities of clinical and environmental isolates of Cryptococcus neoformans in goiânia city, Goiás, Brasil
title_full_unstemmed Antifungal susceptibilities of clinical and environmental isolates of Cryptococcus neoformans in goiânia city, Goiás, Brasil
title_sort Antifungal susceptibilities of clinical and environmental isolates of Cryptococcus neoformans in goiânia city, Goiás, Brasil
author Souza, Lúcia Kioko Hasimoto
author_facet Souza, Lúcia Kioko Hasimoto
Fernandes, Orionalda de Fátima Lisboa
Kobayashi, Cláudia Castelo Branco Artiaga
Passos, Xisto Sena
Costa, Carolina Rodrigues
Lemos, Janine Aquino
Souza-Júnior, Ary Henrique
Silva, Maria do Rosário Rodrigues
author_role author
author2 Fernandes, Orionalda de Fátima Lisboa
Kobayashi, Cláudia Castelo Branco Artiaga
Passos, Xisto Sena
Costa, Carolina Rodrigues
Lemos, Janine Aquino
Souza-Júnior, Ary Henrique
Silva, Maria do Rosário Rodrigues
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Lúcia Kioko Hasimoto
Fernandes, Orionalda de Fátima Lisboa
Kobayashi, Cláudia Castelo Branco Artiaga
Passos, Xisto Sena
Costa, Carolina Rodrigues
Lemos, Janine Aquino
Souza-Júnior, Ary Henrique
Silva, Maria do Rosário Rodrigues
dc.subject.por.fl_str_mv Cryptococcus neoformans
Antifungal agents
Cerebrospinal fluid
Environment
topic Cryptococcus neoformans
Antifungal agents
Cerebrospinal fluid
Environment
description We evaluated the antifungal activities of amphotericin B, fluconazole, itraconazole and voriconazole in 70 Cryptococcus neoformans strains obtained from cerebrospinal fluid from AIDS patients and 40 C. neoformans strains isolated from the environment. Four clinical isolates were identified as C. neoformans var. gattii. The susceptibility test was done using a broth microdilution method according to NCCLS M27-A2. Range minimal inhibitory concentrations (MICs) for C. neoformans clinical isolates were 0.06-1.0 µg/mL for amphotericin B, 0.125-8 µg/mL for fluconazole, 0.03-0.5 µg/mL for itraconazole and 0.03-0.25 µg/mL for voriconazole. C. neoformans environmental isolates showed range MICs 0.015-0.125 µg/mL, 0.25-2.0 µg/mL, 0.007-0.125 µg/mL and 0.03-0.25 µg/mL for amphotericin B, fluconazole, itraconazole and voriconazole respectively. The MICs results obtained from clinical and environmental isolates showed similar pattern of susceptibility and no resistance has been found in our isolates.
publishDate 2005
dc.date.none.fl_str_mv 2005-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30934
url https://www.revistas.usp.br/rimtsp/article/view/30934
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30934/32818
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 47 No. 5 (2005); 253-256
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 47 Núm. 5 (2005); 253-256
Revista do Instituto de Medicina Tropical de São Paulo; v. 47 n. 5 (2005); 253-256
1678-9946
0036-4665
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reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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