Avaliação de genótipos de glicoproteína B e carga de CMV de leucócitos no prognóstico de pacientes de AIDS

Detalhes bibliográficos
Autor(a) principal: Cunha, Aldo Albuquerque
Data de Publicação: 2011
Outros Autores: Aquino, Vitor Hugo, Mariguela, Viviane, Nogueira, Maurício Lacerda, Figueiredo, Luiz Tadeu Moraes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31381
Resumo: BACKGROUND: Cytomegalovirus (CMV) remains an important pathogen to immunocompromised patients even in the era of HAART. The present study aimed at evaluating the influence of CMV viral load and its gB genotypes on AIDS patients' outcome. METHODS: Blood samples of 101 AIDS patients were collected and tested for HIV load, CD4 - cell count and opportunistic pathogens, including CMV. Semi-nested PCRs were run to detect CMV genome and in the positive samples, gB genotyping and CMV load were established using enzymatic restriction and real time PCR, respectively. All patients were clinically followed for four years. RESULTS: In thirty patients (31%) CMV was detected and all fatal cases (n = 5) occurred in this group of patients (p = 0.007), but only two patients had CMV disease (1.9%). However, viral load was not statistically associated with any analyzed parameter. The most frequently observed CMV genotype was gB2 (45.16%) followed by gB3 (35.48%). gB2 genotype was more frequently found in patients with CD4-cell counts under 200 cells/mm³ (p = 0.0017), and almost all fatal cases (80%) had gB2 genotype. CONCLUSIONS: Our study suggests that CMV and its polymorphisms in biologically relevant genes, such as the gB encoding ORF, may still influence the prognosis and outcome of AIDS patients. The gB2 genotype was associated to patient's bad outcome.
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spelling Avaliação de genótipos de glicoproteína B e carga de CMV de leucócitos no prognóstico de pacientes de AIDS Evaluation of glycoprotein B genotypes and load of CMV infecting blood leukocytes on prognosis of AIDS patients CMVCMV loadHIVAIDSCMV genotypes BACKGROUND: Cytomegalovirus (CMV) remains an important pathogen to immunocompromised patients even in the era of HAART. The present study aimed at evaluating the influence of CMV viral load and its gB genotypes on AIDS patients' outcome. METHODS: Blood samples of 101 AIDS patients were collected and tested for HIV load, CD4 - cell count and opportunistic pathogens, including CMV. Semi-nested PCRs were run to detect CMV genome and in the positive samples, gB genotyping and CMV load were established using enzymatic restriction and real time PCR, respectively. All patients were clinically followed for four years. RESULTS: In thirty patients (31%) CMV was detected and all fatal cases (n = 5) occurred in this group of patients (p = 0.007), but only two patients had CMV disease (1.9%). However, viral load was not statistically associated with any analyzed parameter. The most frequently observed CMV genotype was gB2 (45.16%) followed by gB3 (35.48%). gB2 genotype was more frequently found in patients with CD4-cell counts under 200 cells/mm³ (p = 0.0017), and almost all fatal cases (80%) had gB2 genotype. CONCLUSIONS: Our study suggests that CMV and its polymorphisms in biologically relevant genes, such as the gB encoding ORF, may still influence the prognosis and outcome of AIDS patients. The gB2 genotype was associated to patient's bad outcome. ANTECEDENTES: O citomegalovírus (CMV) permanece um importante patógeno para pacientes imunocomprometidos, mesmo na era da HAART. O presente estudo teve como objetivo avaliar a influência da carga viral do CMV e seu genótipo gB sobre a evolução de pacientes com AIDS. MÉTODOS: Amostras de sangue de 101 pacientes com AIDS foram coletadas e testadas para carga viral de HIV, a contagem de células CD4 e patógenos oportunistas, incluindo o CMV. Um sistema de PCRs seminested foi utilizado para detectar o genoma do CMV e em amostras positivas a carga viral de CMV e genotipagem foram estabelecidos por restrição enzimática e PCR em tempo real, respectivamente. Todos os pacientes foram acompanhados clinicamente durante quatro anos. RESULTADOS: Trinta pacientes (31%) tiveram CMV detectado e todos os casos fatais (n = 5) ocorreram em pacientes deste grupo (p = 0,007), porém apenas dois pacientes tinham doença por CMV (1,9%). No entanto, a carga viral não foi associada estatisticamente a nenhum dos parâmetros analisados. O genótipo de CMV mais freqüentemente observado foi gB2 (45,16%), seguido por gB3 (35,48%). O genótipo gB2 foi mais freqüente em pacientes com contagens abaixo de 200 células/mm³ CD4cell (p = 0,0017), e quase todos os casos fatais (80%) tinham o genótipo gB2. CONCLUSÃO: Nosso estudo sugere que CMV e seu polimorfismo em genes relevantes biologicamentes, como a gB, pode ainda influenciar no prognóstico e evolução de pacientes com AIDS. O genótipo gB2 foi associado ao mau prognóstico do paciente. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2011-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31381Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 No. 2 (2011); 82-88 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 Núm. 2 (2011); 82-88 Revista do Instituto de Medicina Tropical de São Paulo; v. 53 n. 2 (2011); 82-88 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31381/33266Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCunha, Aldo AlbuquerqueAquino, Vitor HugoMariguela, VivianeNogueira, Maurício LacerdaFigueiredo, Luiz Tadeu Moraes2012-07-07T19:38:35Zoai:revistas.usp.br:article/31381Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:03.270694Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Avaliação de genótipos de glicoproteína B e carga de CMV de leucócitos no prognóstico de pacientes de AIDS
Evaluation of glycoprotein B genotypes and load of CMV infecting blood leukocytes on prognosis of AIDS patients
title Avaliação de genótipos de glicoproteína B e carga de CMV de leucócitos no prognóstico de pacientes de AIDS
spellingShingle Avaliação de genótipos de glicoproteína B e carga de CMV de leucócitos no prognóstico de pacientes de AIDS
Cunha, Aldo Albuquerque
CMV
CMV load
HIV
AIDS
CMV genotypes
title_short Avaliação de genótipos de glicoproteína B e carga de CMV de leucócitos no prognóstico de pacientes de AIDS
title_full Avaliação de genótipos de glicoproteína B e carga de CMV de leucócitos no prognóstico de pacientes de AIDS
title_fullStr Avaliação de genótipos de glicoproteína B e carga de CMV de leucócitos no prognóstico de pacientes de AIDS
title_full_unstemmed Avaliação de genótipos de glicoproteína B e carga de CMV de leucócitos no prognóstico de pacientes de AIDS
title_sort Avaliação de genótipos de glicoproteína B e carga de CMV de leucócitos no prognóstico de pacientes de AIDS
author Cunha, Aldo Albuquerque
author_facet Cunha, Aldo Albuquerque
Aquino, Vitor Hugo
Mariguela, Viviane
Nogueira, Maurício Lacerda
Figueiredo, Luiz Tadeu Moraes
author_role author
author2 Aquino, Vitor Hugo
Mariguela, Viviane
Nogueira, Maurício Lacerda
Figueiredo, Luiz Tadeu Moraes
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cunha, Aldo Albuquerque
Aquino, Vitor Hugo
Mariguela, Viviane
Nogueira, Maurício Lacerda
Figueiredo, Luiz Tadeu Moraes
dc.subject.por.fl_str_mv CMV
CMV load
HIV
AIDS
CMV genotypes
topic CMV
CMV load
HIV
AIDS
CMV genotypes
description BACKGROUND: Cytomegalovirus (CMV) remains an important pathogen to immunocompromised patients even in the era of HAART. The present study aimed at evaluating the influence of CMV viral load and its gB genotypes on AIDS patients' outcome. METHODS: Blood samples of 101 AIDS patients were collected and tested for HIV load, CD4 - cell count and opportunistic pathogens, including CMV. Semi-nested PCRs were run to detect CMV genome and in the positive samples, gB genotyping and CMV load were established using enzymatic restriction and real time PCR, respectively. All patients were clinically followed for four years. RESULTS: In thirty patients (31%) CMV was detected and all fatal cases (n = 5) occurred in this group of patients (p = 0.007), but only two patients had CMV disease (1.9%). However, viral load was not statistically associated with any analyzed parameter. The most frequently observed CMV genotype was gB2 (45.16%) followed by gB3 (35.48%). gB2 genotype was more frequently found in patients with CD4-cell counts under 200 cells/mm³ (p = 0.0017), and almost all fatal cases (80%) had gB2 genotype. CONCLUSIONS: Our study suggests that CMV and its polymorphisms in biologically relevant genes, such as the gB encoding ORF, may still influence the prognosis and outcome of AIDS patients. The gB2 genotype was associated to patient's bad outcome.
publishDate 2011
dc.date.none.fl_str_mv 2011-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31381
url https://www.revistas.usp.br/rimtsp/article/view/31381
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31381/33266
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 No. 2 (2011); 82-88
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 53 Núm. 2 (2011); 82-88
Revista do Instituto de Medicina Tropical de São Paulo; v. 53 n. 2 (2011); 82-88
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
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reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
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