Genetic relationships among serogroup B: serotype 4 Neisseria meningitidis strains
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30506 |
Resumo: | We compared the results obtained by serotyping of PorB epitopes using an expanded panel of monoclonal antibodies (mAb) including mAb 7 and mAb 10, with results obtained by RFLP of rRNA genes (ribotyping). The purpose of this study was to assess the correlation between phenotypic- and genotypic- methods for typing N. meningitidis. The ribotypes obtained using ClaI or EcoRV endonucleases grouped the strains in seven and two different patterns, respectively. This additional characterization of PorB epitopes improved the correlation between these two methods of typing N. meningitidis. |
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Revista do Instituto de Medicina Tropical de São Paulo |
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Genetic relationships among serogroup B: serotype 4 Neisseria meningitidis strains Relação genética entre cepas de Neisseria meningitidis sorogrupo B sorotipo 4 Neisseria meningitidisSerogroup BSerotype 4RibotypingStrain differentiation We compared the results obtained by serotyping of PorB epitopes using an expanded panel of monoclonal antibodies (mAb) including mAb 7 and mAb 10, with results obtained by RFLP of rRNA genes (ribotyping). The purpose of this study was to assess the correlation between phenotypic- and genotypic- methods for typing N. meningitidis. The ribotypes obtained using ClaI or EcoRV endonucleases grouped the strains in seven and two different patterns, respectively. This additional characterization of PorB epitopes improved the correlation between these two methods of typing N. meningitidis. Nós comparamos os resultados obtidos pela sorotipagem usando um painel de anticorpos monoclonais (mAb) ao qual se incluíam os mAbs 7 e 10, com os resultados obtidos pelo RFLP do gene rRNA (ribotipagem). O propósito deste estudo foi avaliar a correlação entre os métodos fenotípico e genotípico de tipagem de N. meningitidis. Os ribotipos obtidos usando as endonucleases de restrição ClaI e EcoRV foram hábeis em dividir as cepas em sete e dois diferentes perfis de restrição, respectivamente. A caracterização adicional de epítopos na proteína PorB melhorou a correlação entre os dois métodos de tipagem de N. meningitidis. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2001-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30506Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 3 (2001); 119-124 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 3 (2001); 119-124 Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 3 (2001); 119-124 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30506/32390Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessLEMOS, Ana Paula S.SACCHI, Claudio T.PAIVA, Maria V.YARA, Teresa I.MELLES, Carmo Elias A.MAYER, Leonard W.2012-07-07T13:33:13Zoai:revistas.usp.br:article/30506Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:17.388307Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Genetic relationships among serogroup B: serotype 4 Neisseria meningitidis strains Relação genética entre cepas de Neisseria meningitidis sorogrupo B sorotipo 4 |
title |
Genetic relationships among serogroup B: serotype 4 Neisseria meningitidis strains |
spellingShingle |
Genetic relationships among serogroup B: serotype 4 Neisseria meningitidis strains LEMOS, Ana Paula S. Neisseria meningitidis Serogroup B Serotype 4 Ribotyping Strain differentiation |
title_short |
Genetic relationships among serogroup B: serotype 4 Neisseria meningitidis strains |
title_full |
Genetic relationships among serogroup B: serotype 4 Neisseria meningitidis strains |
title_fullStr |
Genetic relationships among serogroup B: serotype 4 Neisseria meningitidis strains |
title_full_unstemmed |
Genetic relationships among serogroup B: serotype 4 Neisseria meningitidis strains |
title_sort |
Genetic relationships among serogroup B: serotype 4 Neisseria meningitidis strains |
author |
LEMOS, Ana Paula S. |
author_facet |
LEMOS, Ana Paula S. SACCHI, Claudio T. PAIVA, Maria V. YARA, Teresa I. MELLES, Carmo Elias A. MAYER, Leonard W. |
author_role |
author |
author2 |
SACCHI, Claudio T. PAIVA, Maria V. YARA, Teresa I. MELLES, Carmo Elias A. MAYER, Leonard W. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
LEMOS, Ana Paula S. SACCHI, Claudio T. PAIVA, Maria V. YARA, Teresa I. MELLES, Carmo Elias A. MAYER, Leonard W. |
dc.subject.por.fl_str_mv |
Neisseria meningitidis Serogroup B Serotype 4 Ribotyping Strain differentiation |
topic |
Neisseria meningitidis Serogroup B Serotype 4 Ribotyping Strain differentiation |
description |
We compared the results obtained by serotyping of PorB epitopes using an expanded panel of monoclonal antibodies (mAb) including mAb 7 and mAb 10, with results obtained by RFLP of rRNA genes (ribotyping). The purpose of this study was to assess the correlation between phenotypic- and genotypic- methods for typing N. meningitidis. The ribotypes obtained using ClaI or EcoRV endonucleases grouped the strains in seven and two different patterns, respectively. This additional characterization of PorB epitopes improved the correlation between these two methods of typing N. meningitidis. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30506 |
url |
https://www.revistas.usp.br/rimtsp/article/view/30506 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30506/32390 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 3 (2001); 119-124 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 3 (2001); 119-124 Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 3 (2001); 119-124 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951642900463617 |