Reinfection of mice and cats by Toxoplasma gondii Nicolle & Manceaux, 1909: experimental study
Autor(a) principal: | |
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Data de Publicação: | 1985 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/87428 |
Resumo: | Experimental reinfection by Toxoplasma gondii in 27 albino mice and 25 domestic cats was studied. Tachyzoites and cysts of AS28 and N strains, by peritoneal and oral route were used. Infection and reinfection were demonstrated by clinical signs, by dye-test and by shedding of oocysts (cats). Mortality of mice was 56%. Infection by the AS28 strain, with low virulence, chronic evolution and cysts in brain, gave some protection to reinfection, manifested by a lower mortality (12.5%) and mice survival. The same occurred when a virulent strain was attenuated by administration of Sulfamonometoxine. Domestic cats were reinfected by ingestion of Toxoplasma cysts, in spite of the presence of a first infection with tachyzoites or cysts by peritoneal or oral via. Mortality was 28% between 4 and 7 1/2 months after infection. After reinfection 48% had higher titers, 44% had unchanged titers and 8% had lower titers. Oocyst shedding after the first infection was 60%, oocyst reshedding was 25%. After the third infection there was no elimination. Shedding of oocysts was observed only after administration of cysts by oral route. Presence of previous serum antibodies of maternal or post-infection origin had no influence neither on immunological response nor on oocysts elimination. Parasites were recovered on 88.2% of the cats and were more frequent in mesenteric lymphonodes and in small intestine; in this organ Toxoplasma was present around 14 months after infection. Wellnouri- shed cats presented ascending titers in dye-test, oocysts shedding in 72% of the animals and reshedding in 30%. Malnourished cats presented low or descending titers, oocysts elimination in 28,5% and no reelimination. Experimental reinfection of cats by Toxoplasma gondii was obtained but the reshedding was infrequent and with fewer oocysts. |
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Reinfection of mice and cats by Toxoplasma gondii Nicolle & Manceaux, 1909: experimental study Reinfecção pelo Toxoplasma gondii Nicolle & Manceaux, 1909 em camundongos e gatos: estudo experimental Experimental reinfection by Toxoplasma gondii in 27 albino mice and 25 domestic cats was studied. Tachyzoites and cysts of AS28 and N strains, by peritoneal and oral route were used. Infection and reinfection were demonstrated by clinical signs, by dye-test and by shedding of oocysts (cats). Mortality of mice was 56%. Infection by the AS28 strain, with low virulence, chronic evolution and cysts in brain, gave some protection to reinfection, manifested by a lower mortality (12.5%) and mice survival. The same occurred when a virulent strain was attenuated by administration of Sulfamonometoxine. Domestic cats were reinfected by ingestion of Toxoplasma cysts, in spite of the presence of a first infection with tachyzoites or cysts by peritoneal or oral via. Mortality was 28% between 4 and 7 1/2 months after infection. After reinfection 48% had higher titers, 44% had unchanged titers and 8% had lower titers. Oocyst shedding after the first infection was 60%, oocyst reshedding was 25%. After the third infection there was no elimination. Shedding of oocysts was observed only after administration of cysts by oral route. Presence of previous serum antibodies of maternal or post-infection origin had no influence neither on immunological response nor on oocysts elimination. Parasites were recovered on 88.2% of the cats and were more frequent in mesenteric lymphonodes and in small intestine; in this organ Toxoplasma was present around 14 months after infection. Wellnouri- shed cats presented ascending titers in dye-test, oocysts shedding in 72% of the animals and reshedding in 30%. Malnourished cats presented low or descending titers, oocysts elimination in 28,5% and no reelimination. Experimental reinfection of cats by Toxoplasma gondii was obtained but the reshedding was infrequent and with fewer oocysts. A reinfecção pelo Toxoplasma gondii foi estudada, experimentalmente, em 27 camundongos albinos e 25 gatos domésticos. A infecção e a reinfecção foram comprovadas por sinais clínicos, anticorpos sangüíneos à reação de Sabin & Feldman >; 1/4, recuperação do parasita e, nos gatos, também pela eliminação de oocistos. Nos camundongos a mortalidade foi de 56%. A primo-infecção por cepa de baixa virulência ou a infecção por cepa mais virulenta, atenuada pela sulfamonometoxina, deram proteção à reinfecção por cepa mais virulenta, mortal em poucos dias. Esta proteção foi demonstrada pela sobrevivência de camundongos e menor mortalidade, 12,5'%. Nos gatos a mortalidade foi de 28%, A resposta sorológica à primo-infecção ocorreu em todos. Após a reinfecção houve elevação de títulos em 64% dos gatos, permanência em 32% e queda em 4%. A eliminação de oocistos foi observada em 60% dos gatos e só após inoculação de cistos, por via digestiva. A reeliminação após reinfecção, ocorreu em 25% dos gatos. Não foi observada eliminação após a 3.ª inoculação. Em 88,2% dos gatos houve recuperação do parasita em camundongo, mais freqüente nos linfonodos mesentéricos e no intestino delgado, persistindo neste cerca de 14 meses. Foi constatada certa relação entre o bom estado físico dos gatos e a resposta sorológica, a eliminação e a reeliminação de oocistos. Apesar da persistência de anticorpos sangüíneos a reinfecção experimental dos gatos foi observada, porém a reeliminação de oocistos foi pouco freqüente e em pequena quantidade. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1985-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/87428Revista do Instituto de Medicina Tropical de São Paulo; Vol. 27 No. 6 (1985); 318-327Revista do Instituto de Medicina Tropical de São Paulo; Vol. 27 Núm. 6 (1985); 318-327Revista do Instituto de Medicina Tropical de São Paulo; v. 27 n. 6 (1985); 318-3271678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/87428/90387Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessJamra, Ligia M. FerreiraSanchis, Fernando SogorbGuimarães, Eny Câmara2015-07-29T17:02:00Zoai:revistas.usp.br:article/87428Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:25.233714Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Reinfection of mice and cats by Toxoplasma gondii Nicolle & Manceaux, 1909: experimental study Reinfecção pelo Toxoplasma gondii Nicolle & Manceaux, 1909 em camundongos e gatos: estudo experimental |
title |
Reinfection of mice and cats by Toxoplasma gondii Nicolle & Manceaux, 1909: experimental study |
spellingShingle |
Reinfection of mice and cats by Toxoplasma gondii Nicolle & Manceaux, 1909: experimental study Jamra, Ligia M. Ferreira |
title_short |
Reinfection of mice and cats by Toxoplasma gondii Nicolle & Manceaux, 1909: experimental study |
title_full |
Reinfection of mice and cats by Toxoplasma gondii Nicolle & Manceaux, 1909: experimental study |
title_fullStr |
Reinfection of mice and cats by Toxoplasma gondii Nicolle & Manceaux, 1909: experimental study |
title_full_unstemmed |
Reinfection of mice and cats by Toxoplasma gondii Nicolle & Manceaux, 1909: experimental study |
title_sort |
Reinfection of mice and cats by Toxoplasma gondii Nicolle & Manceaux, 1909: experimental study |
author |
Jamra, Ligia M. Ferreira |
author_facet |
Jamra, Ligia M. Ferreira Sanchis, Fernando Sogorb Guimarães, Eny Câmara |
author_role |
author |
author2 |
Sanchis, Fernando Sogorb Guimarães, Eny Câmara |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Jamra, Ligia M. Ferreira Sanchis, Fernando Sogorb Guimarães, Eny Câmara |
description |
Experimental reinfection by Toxoplasma gondii in 27 albino mice and 25 domestic cats was studied. Tachyzoites and cysts of AS28 and N strains, by peritoneal and oral route were used. Infection and reinfection were demonstrated by clinical signs, by dye-test and by shedding of oocysts (cats). Mortality of mice was 56%. Infection by the AS28 strain, with low virulence, chronic evolution and cysts in brain, gave some protection to reinfection, manifested by a lower mortality (12.5%) and mice survival. The same occurred when a virulent strain was attenuated by administration of Sulfamonometoxine. Domestic cats were reinfected by ingestion of Toxoplasma cysts, in spite of the presence of a first infection with tachyzoites or cysts by peritoneal or oral via. Mortality was 28% between 4 and 7 1/2 months after infection. After reinfection 48% had higher titers, 44% had unchanged titers and 8% had lower titers. Oocyst shedding after the first infection was 60%, oocyst reshedding was 25%. After the third infection there was no elimination. Shedding of oocysts was observed only after administration of cysts by oral route. Presence of previous serum antibodies of maternal or post-infection origin had no influence neither on immunological response nor on oocysts elimination. Parasites were recovered on 88.2% of the cats and were more frequent in mesenteric lymphonodes and in small intestine; in this organ Toxoplasma was present around 14 months after infection. Wellnouri- shed cats presented ascending titers in dye-test, oocysts shedding in 72% of the animals and reshedding in 30%. Malnourished cats presented low or descending titers, oocysts elimination in 28,5% and no reelimination. Experimental reinfection of cats by Toxoplasma gondii was obtained but the reshedding was infrequent and with fewer oocysts. |
publishDate |
1985 |
dc.date.none.fl_str_mv |
1985-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/87428 |
url |
https://www.revistas.usp.br/rimtsp/article/view/87428 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/87428/90387 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 27 No. 6 (1985); 318-327 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 27 Núm. 6 (1985); 318-327 Revista do Instituto de Medicina Tropical de São Paulo; v. 27 n. 6 (1985); 318-327 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951650136686592 |