Níveis plasmáticos do fator de necrose tumoral-alfa (TNF-alfa) em pacientes com leishmaniose visceral (Calazar). Associação com atividade da doença e remissão clínica com terapia antimonial
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/29348 |
Resumo: | Evaluation of TNF-alpha in patients with Kala-azar has drawn increasing interest due to its regulatory role on the immune system, in addition to its cachetizing activity. The objective of this study was to examine the association between plasma levels of TNF-alpha, measured by immunore-activity (ELISA) and bioactivity (cytotoxicity assay with L-929 cells), and clinical manifestations of visceral leishmaniasis. Plasma samples from 19 patients with Kala-azar were obtained before, during and at the end of antimonial therapy. TNF-alpha determinations was done by using the cytotoxicity assay (all patients) and the enzyme-linked immunoassay (ELISA - 14 patients). A discrepancy between results obtained by ELISA and cytotoxicity assay was observed. Levels of circulating TNF-alpha, assessed by ELISA, were higher in patients than in healthy controls, and declined significantly with improvement in clinical and laboratory parameters. Plasma levels before treatment were 124.7 ± 93.3 pg/ml (mean ± SD) and were higher than at the end of therapy 13.9 ± 25.1 pg/ml (mean ± SD) (p = 0.001). In contrast, plasma levels of TNF-alpha evaluated by cytotoxicity assay did not follow a predicted course during follow-up. Lysis, in this case, might be not totally attributed to TNF-alpha. The discrepancy might be attributed to the presence of factor(s) known to influence the release and activity of TNF-alpha. |
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Níveis plasmáticos do fator de necrose tumoral-alfa (TNF-alfa) em pacientes com leishmaniose visceral (Calazar). Associação com atividade da doença e remissão clínica com terapia antimonial Plasma levels of tumor necrosis factor-alpha in patients with visceral leishmaniasis (Kala-Azar). Association with activity of the disease and clinical remisson following antimonial therapy CytokinesTNF-alphaVisceral Leishmaniasis (Kala-Azar) Evaluation of TNF-alpha in patients with Kala-azar has drawn increasing interest due to its regulatory role on the immune system, in addition to its cachetizing activity. The objective of this study was to examine the association between plasma levels of TNF-alpha, measured by immunore-activity (ELISA) and bioactivity (cytotoxicity assay with L-929 cells), and clinical manifestations of visceral leishmaniasis. Plasma samples from 19 patients with Kala-azar were obtained before, during and at the end of antimonial therapy. TNF-alpha determinations was done by using the cytotoxicity assay (all patients) and the enzyme-linked immunoassay (ELISA - 14 patients). A discrepancy between results obtained by ELISA and cytotoxicity assay was observed. Levels of circulating TNF-alpha, assessed by ELISA, were higher in patients than in healthy controls, and declined significantly with improvement in clinical and laboratory parameters. Plasma levels before treatment were 124.7 ± 93.3 pg/ml (mean ± SD) and were higher than at the end of therapy 13.9 ± 25.1 pg/ml (mean ± SD) (p = 0.001). In contrast, plasma levels of TNF-alpha evaluated by cytotoxicity assay did not follow a predicted course during follow-up. Lysis, in this case, might be not totally attributed to TNF-alpha. The discrepancy might be attributed to the presence of factor(s) known to influence the release and activity of TNF-alpha. Avaliação de TNF-alfa em pacientes com calazar tem despertado grande interesse devido ao seu papel no sistema imunológico e à sua atividade caquetizante. O objetivo deste estudo foi examinar a associação entre os níveis plasmáticos de TNF-alfa, medidos através de sua imunorreatividade (ELISA) e bioatividade (ensaio citotóxico sobre as células L-929), e as manifestações clínicas da leishmaniose visceral. Amostras de 19 pacientes foram obtidas para determinação do TNF-alfa antes, durante e após a terapia antimonial, utilizando o ensaio de citotoxicidade (todos os pacientes) e o ELISA (14 pacientes). Resultados discrepantes entre os ensaios de citotoxicidade e o ELISA foram observados. Níveis circulantes de TNF-alfa, medidos pelo ELISA, foram mais altos nos pacientes que nos controles e declinaram significantemente com a melhora clínica e laboratorial. Níveis plasmáticos antes do tratamento (média = 124,7 pg/ml; DP = 93,3) foram mais elevados que ao final da terapêutica (13,9 pg/ml; DP = 25,1; p = 0,001). Por outro lado, níveis plasmáticos de TNF-alfa, avaliados pela citotoxicidade, não seguiram um curso previsível durante a evolução. Esta discrepância pode ser devida à presença de fatores no plasma que podem influenciar a liberação e atividade do TNF-alfa. Ainda, a lise observada pode não ser totalmente atribuída ao TNF-alfa. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1996-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29348Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 No. 2 (1996); 113-118 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 Núm. 2 (1996); 113-118 Revista do Instituto de Medicina Tropical de São Paulo; v. 38 n. 2 (1996); 113-118 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29348/31205Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessSalomão, ReinaldoCastelo Filho, AdautoMedeiros, Iara Marques deSicolo, Miguel Angel2012-07-02T01:43:16Zoai:revistas.usp.br:article/29348Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:05.830289Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Níveis plasmáticos do fator de necrose tumoral-alfa (TNF-alfa) em pacientes com leishmaniose visceral (Calazar). Associação com atividade da doença e remissão clínica com terapia antimonial Plasma levels of tumor necrosis factor-alpha in patients with visceral leishmaniasis (Kala-Azar). Association with activity of the disease and clinical remisson following antimonial therapy |
title |
Níveis plasmáticos do fator de necrose tumoral-alfa (TNF-alfa) em pacientes com leishmaniose visceral (Calazar). Associação com atividade da doença e remissão clínica com terapia antimonial |
spellingShingle |
Níveis plasmáticos do fator de necrose tumoral-alfa (TNF-alfa) em pacientes com leishmaniose visceral (Calazar). Associação com atividade da doença e remissão clínica com terapia antimonial Salomão, Reinaldo Cytokines TNF-alpha Visceral Leishmaniasis (Kala-Azar) |
title_short |
Níveis plasmáticos do fator de necrose tumoral-alfa (TNF-alfa) em pacientes com leishmaniose visceral (Calazar). Associação com atividade da doença e remissão clínica com terapia antimonial |
title_full |
Níveis plasmáticos do fator de necrose tumoral-alfa (TNF-alfa) em pacientes com leishmaniose visceral (Calazar). Associação com atividade da doença e remissão clínica com terapia antimonial |
title_fullStr |
Níveis plasmáticos do fator de necrose tumoral-alfa (TNF-alfa) em pacientes com leishmaniose visceral (Calazar). Associação com atividade da doença e remissão clínica com terapia antimonial |
title_full_unstemmed |
Níveis plasmáticos do fator de necrose tumoral-alfa (TNF-alfa) em pacientes com leishmaniose visceral (Calazar). Associação com atividade da doença e remissão clínica com terapia antimonial |
title_sort |
Níveis plasmáticos do fator de necrose tumoral-alfa (TNF-alfa) em pacientes com leishmaniose visceral (Calazar). Associação com atividade da doença e remissão clínica com terapia antimonial |
author |
Salomão, Reinaldo |
author_facet |
Salomão, Reinaldo Castelo Filho, Adauto Medeiros, Iara Marques de Sicolo, Miguel Angel |
author_role |
author |
author2 |
Castelo Filho, Adauto Medeiros, Iara Marques de Sicolo, Miguel Angel |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Salomão, Reinaldo Castelo Filho, Adauto Medeiros, Iara Marques de Sicolo, Miguel Angel |
dc.subject.por.fl_str_mv |
Cytokines TNF-alpha Visceral Leishmaniasis (Kala-Azar) |
topic |
Cytokines TNF-alpha Visceral Leishmaniasis (Kala-Azar) |
description |
Evaluation of TNF-alpha in patients with Kala-azar has drawn increasing interest due to its regulatory role on the immune system, in addition to its cachetizing activity. The objective of this study was to examine the association between plasma levels of TNF-alpha, measured by immunore-activity (ELISA) and bioactivity (cytotoxicity assay with L-929 cells), and clinical manifestations of visceral leishmaniasis. Plasma samples from 19 patients with Kala-azar were obtained before, during and at the end of antimonial therapy. TNF-alpha determinations was done by using the cytotoxicity assay (all patients) and the enzyme-linked immunoassay (ELISA - 14 patients). A discrepancy between results obtained by ELISA and cytotoxicity assay was observed. Levels of circulating TNF-alpha, assessed by ELISA, were higher in patients than in healthy controls, and declined significantly with improvement in clinical and laboratory parameters. Plasma levels before treatment were 124.7 ± 93.3 pg/ml (mean ± SD) and were higher than at the end of therapy 13.9 ± 25.1 pg/ml (mean ± SD) (p = 0.001). In contrast, plasma levels of TNF-alpha evaluated by cytotoxicity assay did not follow a predicted course during follow-up. Lysis, in this case, might be not totally attributed to TNF-alpha. The discrepancy might be attributed to the presence of factor(s) known to influence the release and activity of TNF-alpha. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29348 |
url |
https://www.revistas.usp.br/rimtsp/article/view/29348 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29348/31205 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 No. 2 (1996); 113-118 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 38 Núm. 2 (1996); 113-118 Revista do Instituto de Medicina Tropical de São Paulo; v. 38 n. 2 (1996); 113-118 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951641737592832 |