Report of autochthonous cases of localized cutaneous leishmaniasis caused by Leishmania (Leishmania) mexicana in vulnerable, susceptible areas of Southeastern Mexico
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/198246 |
Resumo: | Localized cutaneous leishmaniasis (LCL) is an endemic disease in several Mexican States with the main endemic areas located in the South-Southeast region of the country, where 90% of Leishmania (Leishmania) mexicana cases are registered. The Southeast region is located in the Yucatan Peninsula, including Campeche, Quintana Roo and Yucatan States. Campeche and Quintana Roo register more than 60% of the cases in the country each year, while in Yucatan the reports are of imported cases due to residents traveling to endemic areas. However, since 2015, autochthonous cases have been diagnosed by health authorities in municipalities with no previous transmission records. We aimed to identify Leishmania parasite species involved in autochthonous cases by means of the PCR technique. The present study included 13 autochthonous cases of LCL with clinical and parasitological diagnoses during 2018 and 2019 by health authorities, without specific identification of the causal agent. Tissue samples were taken by scraping the margins of active lesions and then they were spotted onto an FTATM Elute Microcard. Next, DNA was eluted and used for PCR amplification of specific Leishmania genus and L. (L.) mexicana species-specific fragments. Molecular analysis showed evidence that L. (L.) mexicana was the causal agent of LCL in 12 of the 13 patients; in one patient, PCR was not performed due to the patient’s refusal to participate in the study. Identifying Leishmania species that cause LCL is necessary to define efficient treatment schemes and control strategies for the disease in vulnerable and susceptible areas of the Yucatan State’s municipalities. |
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Report of autochthonous cases of localized cutaneous leishmaniasis caused by Leishmania (Leishmania) mexicana in vulnerable, susceptible areas of Southeastern MexicoLeishmaniasisLeishmania (Leishmania) mexicanaAutochthonous casesEmergenceNeglected tropical diseasesSpecies-specific PCRLocalized cutaneous leishmaniasis (LCL) is an endemic disease in several Mexican States with the main endemic areas located in the South-Southeast region of the country, where 90% of Leishmania (Leishmania) mexicana cases are registered. The Southeast region is located in the Yucatan Peninsula, including Campeche, Quintana Roo and Yucatan States. Campeche and Quintana Roo register more than 60% of the cases in the country each year, while in Yucatan the reports are of imported cases due to residents traveling to endemic areas. However, since 2015, autochthonous cases have been diagnosed by health authorities in municipalities with no previous transmission records. We aimed to identify Leishmania parasite species involved in autochthonous cases by means of the PCR technique. The present study included 13 autochthonous cases of LCL with clinical and parasitological diagnoses during 2018 and 2019 by health authorities, without specific identification of the causal agent. Tissue samples were taken by scraping the margins of active lesions and then they were spotted onto an FTATM Elute Microcard. Next, DNA was eluted and used for PCR amplification of specific Leishmania genus and L. (L.) mexicana species-specific fragments. Molecular analysis showed evidence that L. (L.) mexicana was the causal agent of LCL in 12 of the 13 patients; in one patient, PCR was not performed due to the patient’s refusal to participate in the study. Identifying Leishmania species that cause LCL is necessary to define efficient treatment schemes and control strategies for the disease in vulnerable and susceptible areas of the Yucatan State’s municipalities.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2022-05-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/19824610.1590/S1678-9946202264035 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e35Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e35Revista do Instituto de Medicina Tropical de São Paulo; v. 64 (2022); e351678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/198246/182347Copyright (c) 2022 Elsy Beatriz Canché-Pool, Dulce María Canto-Hau, Mirna Alexandra Vargas-Meléndez, Raúl Tello-Martín, Enrique Reyes-Novelo, Francisco Javier Escobedo-Ortegón, Hugo Antonio Ruiz-Piña, Luis Humberto Cambranes-Puc, Jimmy Raymundo Torres-Castro, Jorge Alfredo Palacio-Vargas, Celmy Durán-Caamal, José Cerón-Espinosa, Juan Carlos Carpio-Pedroza, Octavio César Rivera-Hernándezhttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCanché-Pool, Elsy Beatriz Canto-Hau, Dulce María Vargas-Meléndez, Mirna Alexandra Tello-Martín, Raúl Reyes-Novelo, Enrique Escobedo-Ortegón, Francisco Javier Ruiz-Piña, Hugo Antonio Cambranes-Puc, Luis Humberto Torres-Castro, Jimmy Raymundo Palacio-Vargas, Jorge Alfredo Durán-Caamal, Celmy Cerón-Espinosa, José Carpio-Pedroza, Juan Carlos Rivera-Hernández, Octavio César 2022-10-10T13:01:46Zoai:revistas.usp.br:article/198246Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:53:24.497760Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Report of autochthonous cases of localized cutaneous leishmaniasis caused by Leishmania (Leishmania) mexicana in vulnerable, susceptible areas of Southeastern Mexico |
title |
Report of autochthonous cases of localized cutaneous leishmaniasis caused by Leishmania (Leishmania) mexicana in vulnerable, susceptible areas of Southeastern Mexico |
spellingShingle |
Report of autochthonous cases of localized cutaneous leishmaniasis caused by Leishmania (Leishmania) mexicana in vulnerable, susceptible areas of Southeastern Mexico Canché-Pool, Elsy Beatriz Leishmaniasis Leishmania (Leishmania) mexicana Autochthonous cases Emergence Neglected tropical diseases Species-specific PCR |
title_short |
Report of autochthonous cases of localized cutaneous leishmaniasis caused by Leishmania (Leishmania) mexicana in vulnerable, susceptible areas of Southeastern Mexico |
title_full |
Report of autochthonous cases of localized cutaneous leishmaniasis caused by Leishmania (Leishmania) mexicana in vulnerable, susceptible areas of Southeastern Mexico |
title_fullStr |
Report of autochthonous cases of localized cutaneous leishmaniasis caused by Leishmania (Leishmania) mexicana in vulnerable, susceptible areas of Southeastern Mexico |
title_full_unstemmed |
Report of autochthonous cases of localized cutaneous leishmaniasis caused by Leishmania (Leishmania) mexicana in vulnerable, susceptible areas of Southeastern Mexico |
title_sort |
Report of autochthonous cases of localized cutaneous leishmaniasis caused by Leishmania (Leishmania) mexicana in vulnerable, susceptible areas of Southeastern Mexico |
author |
Canché-Pool, Elsy Beatriz |
author_facet |
Canché-Pool, Elsy Beatriz Canto-Hau, Dulce María Vargas-Meléndez, Mirna Alexandra Tello-Martín, Raúl Reyes-Novelo, Enrique Escobedo-Ortegón, Francisco Javier Ruiz-Piña, Hugo Antonio Cambranes-Puc, Luis Humberto Torres-Castro, Jimmy Raymundo Palacio-Vargas, Jorge Alfredo Durán-Caamal, Celmy Cerón-Espinosa, José Carpio-Pedroza, Juan Carlos Rivera-Hernández, Octavio César |
author_role |
author |
author2 |
Canto-Hau, Dulce María Vargas-Meléndez, Mirna Alexandra Tello-Martín, Raúl Reyes-Novelo, Enrique Escobedo-Ortegón, Francisco Javier Ruiz-Piña, Hugo Antonio Cambranes-Puc, Luis Humberto Torres-Castro, Jimmy Raymundo Palacio-Vargas, Jorge Alfredo Durán-Caamal, Celmy Cerón-Espinosa, José Carpio-Pedroza, Juan Carlos Rivera-Hernández, Octavio César |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Canché-Pool, Elsy Beatriz Canto-Hau, Dulce María Vargas-Meléndez, Mirna Alexandra Tello-Martín, Raúl Reyes-Novelo, Enrique Escobedo-Ortegón, Francisco Javier Ruiz-Piña, Hugo Antonio Cambranes-Puc, Luis Humberto Torres-Castro, Jimmy Raymundo Palacio-Vargas, Jorge Alfredo Durán-Caamal, Celmy Cerón-Espinosa, José Carpio-Pedroza, Juan Carlos Rivera-Hernández, Octavio César |
dc.subject.por.fl_str_mv |
Leishmaniasis Leishmania (Leishmania) mexicana Autochthonous cases Emergence Neglected tropical diseases Species-specific PCR |
topic |
Leishmaniasis Leishmania (Leishmania) mexicana Autochthonous cases Emergence Neglected tropical diseases Species-specific PCR |
description |
Localized cutaneous leishmaniasis (LCL) is an endemic disease in several Mexican States with the main endemic areas located in the South-Southeast region of the country, where 90% of Leishmania (Leishmania) mexicana cases are registered. The Southeast region is located in the Yucatan Peninsula, including Campeche, Quintana Roo and Yucatan States. Campeche and Quintana Roo register more than 60% of the cases in the country each year, while in Yucatan the reports are of imported cases due to residents traveling to endemic areas. However, since 2015, autochthonous cases have been diagnosed by health authorities in municipalities with no previous transmission records. We aimed to identify Leishmania parasite species involved in autochthonous cases by means of the PCR technique. The present study included 13 autochthonous cases of LCL with clinical and parasitological diagnoses during 2018 and 2019 by health authorities, without specific identification of the causal agent. Tissue samples were taken by scraping the margins of active lesions and then they were spotted onto an FTATM Elute Microcard. Next, DNA was eluted and used for PCR amplification of specific Leishmania genus and L. (L.) mexicana species-specific fragments. Molecular analysis showed evidence that L. (L.) mexicana was the causal agent of LCL in 12 of the 13 patients; in one patient, PCR was not performed due to the patient’s refusal to participate in the study. Identifying Leishmania species that cause LCL is necessary to define efficient treatment schemes and control strategies for the disease in vulnerable and susceptible areas of the Yucatan State’s municipalities. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-26 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/198246 10.1590/S1678-9946202264035 |
url |
https://www.revistas.usp.br/rimtsp/article/view/198246 |
identifier_str_mv |
10.1590/S1678-9946202264035 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/198246/182347 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e35 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e35 Revista do Instituto de Medicina Tropical de São Paulo; v. 64 (2022); e35 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951655707770880 |