Discrepancies and consequences of indirect hemagglutination, indirect immunofluorescence and Elisa tests for the diagnosis of Chagas disease

Detalhes bibliográficos
Autor(a) principal: Souza, Regina Maia de
Data de Publicação: 2012
Outros Autores: Amato Neto, Vicente
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31467
Resumo: Using the indirect hemagglutination (IH), indirect immunofluorescence (IIF) and enzyme linked immunosorbent assay (ELISA) tests for the diagnosis of Chagas disease, 4000 serum samples were examined. This study was conducted with different purposes: clinical interest, research support and parasitological monitoring of those patients with Chagas disease who were treated with heart transplantations. The tests occurred without patient selection and in accordance with the medical requests. The results showed discrepancies and brought about several questions, considering the different results that all three methods showed when considered together. What was found brought about concerns and we suggest the adoption of different measures, aiming to avoid these mismatches in the context of this disease.
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spelling Discrepancies and consequences of indirect hemagglutination, indirect immunofluorescence and Elisa tests for the diagnosis of Chagas disease Discordâncias e consequências de resultados de provas de hemaglutinação indireta, imunofluorescência indireta e Elisa para o diagnóstico da doença de Chagas Chagas diseaseSerological diagnosisDiscrepanciesConsequences Using the indirect hemagglutination (IH), indirect immunofluorescence (IIF) and enzyme linked immunosorbent assay (ELISA) tests for the diagnosis of Chagas disease, 4000 serum samples were examined. This study was conducted with different purposes: clinical interest, research support and parasitological monitoring of those patients with Chagas disease who were treated with heart transplantations. The tests occurred without patient selection and in accordance with the medical requests. The results showed discrepancies and brought about several questions, considering the different results that all three methods showed when considered together. What was found brought about concerns and we suggest the adoption of different measures, aiming to avoid these mismatches in the context of this disease. Com as provas de hemaglutinação indireta (HI), imunofluorescência indireta (IFI) e Enzyme Linked Immunosorbent Assay (ELISA), para diagnóstico da doença de Chagas, foram examinadas concomitantemente 4000 amostras de soro, com diferentes finalidades, tais como interesse clínico, apoio a pesquisas e acompanhamento parasitológico de pacientes com tal moléstia tratados por meio de transplante de coração. Os testes ocorreram, sem seleção e conforme as solicitações, em Laboratório que essencialmente prestou colaboração. Os resultados mostraram discordâncias, inclusive motivadoras de dúvidas, considerando especificamente o revelado pelos três métodos em conjunto. O que ficou verificado suscita preocupações e sugere a adoção de medidas aptas a evitar essas inadequações no contexto da parasitose. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31467Revista do Instituto de Medicina Tropical de São Paulo; Vol. 54 No. 3 (2012); 141-144 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 54 Núm. 3 (2012); 141-144 Revista do Instituto de Medicina Tropical de São Paulo; v. 54 n. 3 (2012); 141-144 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31467/33352Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessSouza, Regina Maia deAmato Neto, Vicente2012-07-07T19:46:07Zoai:revistas.usp.br:article/31467Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:08.695683Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Discrepancies and consequences of indirect hemagglutination, indirect immunofluorescence and Elisa tests for the diagnosis of Chagas disease
Discordâncias e consequências de resultados de provas de hemaglutinação indireta, imunofluorescência indireta e Elisa para o diagnóstico da doença de Chagas
title Discrepancies and consequences of indirect hemagglutination, indirect immunofluorescence and Elisa tests for the diagnosis of Chagas disease
spellingShingle Discrepancies and consequences of indirect hemagglutination, indirect immunofluorescence and Elisa tests for the diagnosis of Chagas disease
Souza, Regina Maia de
Chagas disease
Serological diagnosis
Discrepancies
Consequences
title_short Discrepancies and consequences of indirect hemagglutination, indirect immunofluorescence and Elisa tests for the diagnosis of Chagas disease
title_full Discrepancies and consequences of indirect hemagglutination, indirect immunofluorescence and Elisa tests for the diagnosis of Chagas disease
title_fullStr Discrepancies and consequences of indirect hemagglutination, indirect immunofluorescence and Elisa tests for the diagnosis of Chagas disease
title_full_unstemmed Discrepancies and consequences of indirect hemagglutination, indirect immunofluorescence and Elisa tests for the diagnosis of Chagas disease
title_sort Discrepancies and consequences of indirect hemagglutination, indirect immunofluorescence and Elisa tests for the diagnosis of Chagas disease
author Souza, Regina Maia de
author_facet Souza, Regina Maia de
Amato Neto, Vicente
author_role author
author2 Amato Neto, Vicente
author2_role author
dc.contributor.author.fl_str_mv Souza, Regina Maia de
Amato Neto, Vicente
dc.subject.por.fl_str_mv Chagas disease
Serological diagnosis
Discrepancies
Consequences
topic Chagas disease
Serological diagnosis
Discrepancies
Consequences
description Using the indirect hemagglutination (IH), indirect immunofluorescence (IIF) and enzyme linked immunosorbent assay (ELISA) tests for the diagnosis of Chagas disease, 4000 serum samples were examined. This study was conducted with different purposes: clinical interest, research support and parasitological monitoring of those patients with Chagas disease who were treated with heart transplantations. The tests occurred without patient selection and in accordance with the medical requests. The results showed discrepancies and brought about several questions, considering the different results that all three methods showed when considered together. What was found brought about concerns and we suggest the adoption of different measures, aiming to avoid these mismatches in the context of this disease.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31467
url https://www.revistas.usp.br/rimtsp/article/view/31467
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31467/33352
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 54 No. 3 (2012); 141-144
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 54 Núm. 3 (2012); 141-144
Revista do Instituto de Medicina Tropical de São Paulo; v. 54 n. 3 (2012); 141-144
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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