Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2002 |
| Outros Autores: | , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
| Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30634 |
Resumo: | In the present study, we have analyzed by sodium docecyl sulphate - polyacrilamide gel electrophoresis (SDS-PAGE), immunoblotting and Concanavalin A blotting (Con A blotting) proteins of membrane fractions and soluble fractions obtained from Giardia duodenalis trophozoites of two axenic strains isolated in Brazil from a symptomatic (BTU-11) and an asymptomatic patient (BTU-10), as compared to the reference strain Portland 1. Both Brazilian strains showed a complex and homogeneous electrophoretic pattern of proteins, but some differences could be observed. Several glycoproteins were detected, particularly the proteins of 81, 72, 59 kDa and the protein of 62 kDa in the membrane proteins and cytosol, respectively. Many antigenic components were revealed by anti-Giardia rabbit IgG antibodies in the immunoblotting analysis. Among these components, the membrane protein of 32 kDa and the cytosol protein of 30 kDa could be related to giardin, as previously demonstrated. |
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Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains Análise de proteínas de frações de membranas e de frações solúveis de trofozoítos de duas cepas de Giardia duodenalis, isoladas e axenizadas no Brasil Giardia duodenalisAxenic strainsTrophozoitesProteinsGlycoproteinsAntigens In the present study, we have analyzed by sodium docecyl sulphate - polyacrilamide gel electrophoresis (SDS-PAGE), immunoblotting and Concanavalin A blotting (Con A blotting) proteins of membrane fractions and soluble fractions obtained from Giardia duodenalis trophozoites of two axenic strains isolated in Brazil from a symptomatic (BTU-11) and an asymptomatic patient (BTU-10), as compared to the reference strain Portland 1. Both Brazilian strains showed a complex and homogeneous electrophoretic pattern of proteins, but some differences could be observed. Several glycoproteins were detected, particularly the proteins of 81, 72, 59 kDa and the protein of 62 kDa in the membrane proteins and cytosol, respectively. Many antigenic components were revealed by anti-Giardia rabbit IgG antibodies in the immunoblotting analysis. Among these components, the membrane protein of 32 kDa and the cytosol protein of 30 kDa could be related to giardin, as previously demonstrated. Foi realizada a análise de algumas características bioquímicas e antigênicas de duas cepas axênicas de Giardia duodenalis, isoladas em Botucatu, a partir de cistos obtidos de paciente sintomático (BTU-11) e assintomático (BTU-10), tendo por referência a cepa padrão Portland 1 (P-1), isolada e axenizada nos Estados Unidos. Preparações contendo proteínas de membranas e proteínas solúveis (citosol) de cada cepa foram empregadas na eletroforese das proteínas em gel de poliacrilamida (SDS-PAGE), na análise de glicoproteínas pela técnica de Con A blot e na análise antigênica pela técnica de "Immunoblotting". Na análise do perfil eletroforético os padrões de bandas de proteínas das cepas BTU-10 e BTU-11 foram complexos e na maior parte homogêneos; algumas diferenças foram detectadas entre as proteínas de membranas. Várias glicoproteínas foram demonstradas, destacando-se as de 81, 72, 59 kDa e a glicoproteína de 62 kDa, detectadas nas preparações de proteínas de membranas e proteínas solúveis, respectivamente. Entre estas glicoproteínas, a de 81 kDa foi detectada apenas entre as proteínas de membranas das cepas BTU-11 e P-1, isoladas de casos sintomáticos. Vários componentes antigênicos foram reconhecidos por anticorpos IgG de coelho pela técnica de "Immunoblotting". Entre estes componentes, as proteínas de 32 kDa, entre as proteínas de membranas e a de 30 kDa, entre as proteínas solúveis, parecem tratar-se de proteínas já caracterizadas como "giardinas". O presente trabalho possibilitou detectar glicoproteínas, proteínas e antígenos em trofozoítos de duas cepas autóctones de G. duodenalis. De modo geral, as análises dos perfis eletroforético e glicoproteico e do "immunoblotting" demonstraram similaridade entre as cepas. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2002-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30634Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 No. 5 (2002); 239-244 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 Núm. 5 (2002); 239-244 Revista do Instituto de Medicina Tropical de São Paulo; v. 44 n. 5 (2002); 239-244 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30634/32518Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessGUIMARÃES, SemíramisSOGAYAR, Maria Inês T. LemeFRANCO, Marcello2012-07-07T17:52:37Zoai:revistas.usp.br:article/30634Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:24.582059Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
| dc.title.none.fl_str_mv |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains Análise de proteínas de frações de membranas e de frações solúveis de trofozoítos de duas cepas de Giardia duodenalis, isoladas e axenizadas no Brasil |
| title |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains |
| spellingShingle |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains GUIMARÃES, Semíramis Giardia duodenalis Axenic strains Trophozoites Proteins Glycoproteins Antigens |
| title_short |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains |
| title_full |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains |
| title_fullStr |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains |
| title_full_unstemmed |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains |
| title_sort |
Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains |
| author |
GUIMARÃES, Semíramis |
| author_facet |
GUIMARÃES, Semíramis SOGAYAR, Maria Inês T. Leme FRANCO, Marcello |
| author_role |
author |
| author2 |
SOGAYAR, Maria Inês T. Leme FRANCO, Marcello |
| author2_role |
author author |
| dc.contributor.author.fl_str_mv |
GUIMARÃES, Semíramis SOGAYAR, Maria Inês T. Leme FRANCO, Marcello |
| dc.subject.por.fl_str_mv |
Giardia duodenalis Axenic strains Trophozoites Proteins Glycoproteins Antigens |
| topic |
Giardia duodenalis Axenic strains Trophozoites Proteins Glycoproteins Antigens |
| description |
In the present study, we have analyzed by sodium docecyl sulphate - polyacrilamide gel electrophoresis (SDS-PAGE), immunoblotting and Concanavalin A blotting (Con A blotting) proteins of membrane fractions and soluble fractions obtained from Giardia duodenalis trophozoites of two axenic strains isolated in Brazil from a symptomatic (BTU-11) and an asymptomatic patient (BTU-10), as compared to the reference strain Portland 1. Both Brazilian strains showed a complex and homogeneous electrophoretic pattern of proteins, but some differences could be observed. Several glycoproteins were detected, particularly the proteins of 81, 72, 59 kDa and the protein of 62 kDa in the membrane proteins and cytosol, respectively. Many antigenic components were revealed by anti-Giardia rabbit IgG antibodies in the immunoblotting analysis. Among these components, the membrane protein of 32 kDa and the cytosol protein of 30 kDa could be related to giardin, as previously demonstrated. |
| publishDate |
2002 |
| dc.date.none.fl_str_mv |
2002-10-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30634 |
| url |
https://www.revistas.usp.br/rimtsp/article/view/30634 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30634/32518 |
| dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
| publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
| dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 No. 5 (2002); 239-244 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 44 Núm. 5 (2002); 239-244 Revista do Instituto de Medicina Tropical de São Paulo; v. 44 n. 5 (2002); 239-244 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
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Instituto de Medicina Tropical (IMT) |
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IMT |
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IMT |
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Revista do Instituto de Medicina Tropical de São Paulo |
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Revista do Instituto de Medicina Tropical de São Paulo |
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Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
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1798951643467743232 |