Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/31114 |
Resumo: | PURPOSE: The aim of this study was to investigate the frequency of HPV infection and the types 16 and 18 in cervical samples from patients attended at two public health services of the city of Belo Horizonte, MG. METHODS: Cervical samples from 174 patients were collected for cytopathological and molecular tests. HPV infection was searched by PCR utilizing MY09 and MY11 primers or HPV 16 and HPV 18 specific primers. RESULTS: Amongst the 174 samples analyzed, 20.7% presented squamous intraepithelial and/or invasive lesions detected on cytopathological analysis and of those, 94.4% were infected by HPV. HPV 16 was found in 20% of the cases of low-grade squamous intraepithelial lesions and in 40% and 50% of high-grade squamous intraepithelial lesion and squamous invasive carcinoma, respectively. HPV 18 was detected in 6.7% of the low-grade lesion samples and in two HPV16 co-infected samples. In 50% of the cases of high-grade lesion, the HPV type was not determined. CONCLUSION: The HPV 16 was the virus type more frequently detected. However, more than 50% of the positive samples at the cytopathological analysis were negative for HPV 16 and 18, indicating that possibly other virus types are present in relative high frequencies in the studied population. |
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Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil Molecular detection of HPV 16 and 18 in cervical samples of patients from Belo Horizonte, Minas Gerais, Brazil HPVCervical cancerPCRGenotypingCytology PURPOSE: The aim of this study was to investigate the frequency of HPV infection and the types 16 and 18 in cervical samples from patients attended at two public health services of the city of Belo Horizonte, MG. METHODS: Cervical samples from 174 patients were collected for cytopathological and molecular tests. HPV infection was searched by PCR utilizing MY09 and MY11 primers or HPV 16 and HPV 18 specific primers. RESULTS: Amongst the 174 samples analyzed, 20.7% presented squamous intraepithelial and/or invasive lesions detected on cytopathological analysis and of those, 94.4% were infected by HPV. HPV 16 was found in 20% of the cases of low-grade squamous intraepithelial lesions and in 40% and 50% of high-grade squamous intraepithelial lesion and squamous invasive carcinoma, respectively. HPV 18 was detected in 6.7% of the low-grade lesion samples and in two HPV16 co-infected samples. In 50% of the cases of high-grade lesion, the HPV type was not determined. CONCLUSION: The HPV 16 was the virus type more frequently detected. However, more than 50% of the positive samples at the cytopathological analysis were negative for HPV 16 and 18, indicating that possibly other virus types are present in relative high frequencies in the studied population. OBJETIVOS: O objetivo deste estudo foi investigar a freqüência da infecção por HPV e dos tipos 16 e 18 em amostras cervicais de pacientes atendidas em dois serviços públicos da cidade de Belo Horizonte-MG. MÉTODOS: Amostras cervicais de 174 pacientes foram coletadas para estudo citopatológico e molecular. A pesquisa da infecção por HPV foi feita através da PCR utilizando os oligonucleotídeos MY09/MY11. Os tipos virais 16 e 18 foram pesquisados através da utilização de oligonucleotídeos específicos. RESULTADOS: Dentre as 174 amostras analisadas, 20,7% apresentaram lesões escamosas intra-epiteliais e/ou invasoras detectadas na análise citopatológica, das quais 94,4% mostraram infecção por HPV. O HPV 16 foi encontrado em torno de 20% dos casos de lesão escamosa intra-epitelial de baixo grau e em 40% e 50% dos casos de lesão escamosa intra-epitelial de alto grau e carcinoma escamoso invasor, respectivamente. O HPV 18 foi encontrado em 6,7% das amostras com lesão de baixo grau e em dois casos de co-infecção com HPV 16. Em 50% dos casos de lesão de alto grau, o tipo de HPV não foi determinado. CONCLUSÕES: O HPV 16 foi o tipo viral mais freqüentemente detectado. No entanto, mais de 50% das amostras positivas no exame citopatológico não apresentaram HPV 16 e 18, indicando que possivelmente outros tipos virais estejam presentes em freqüências relativamente altas na população estudada. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2007-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31114Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 No. 5 (2007); 297-301 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 Núm. 5 (2007); 297-301 Revista do Instituto de Medicina Tropical de São Paulo; v. 49 n. 5 (2007); 297-301 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31114/32998Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessFreitas, Taíse PalmeirasCarmo, Bianca Bianco doPaula, Francisco Danilo FerreiraRodrigues, Lucas FonsecaFernandes, Ana PaulaFernandes, Paula Ávila2012-07-07T19:03:44Zoai:revistas.usp.br:article/31114Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:48.735440Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil Molecular detection of HPV 16 and 18 in cervical samples of patients from Belo Horizonte, Minas Gerais, Brazil |
title |
Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil |
spellingShingle |
Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil Freitas, Taíse Palmeiras HPV Cervical cancer PCR Genotyping Cytology |
title_short |
Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil |
title_full |
Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil |
title_fullStr |
Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil |
title_full_unstemmed |
Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil |
title_sort |
Detecção molecular de HPV 16 e 18 em amostras cervicais de pacientes de Belo Horizonte, Minas Gerais, Brasil |
author |
Freitas, Taíse Palmeiras |
author_facet |
Freitas, Taíse Palmeiras Carmo, Bianca Bianco do Paula, Francisco Danilo Ferreira Rodrigues, Lucas Fonseca Fernandes, Ana Paula Fernandes, Paula Ávila |
author_role |
author |
author2 |
Carmo, Bianca Bianco do Paula, Francisco Danilo Ferreira Rodrigues, Lucas Fonseca Fernandes, Ana Paula Fernandes, Paula Ávila |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Freitas, Taíse Palmeiras Carmo, Bianca Bianco do Paula, Francisco Danilo Ferreira Rodrigues, Lucas Fonseca Fernandes, Ana Paula Fernandes, Paula Ávila |
dc.subject.por.fl_str_mv |
HPV Cervical cancer PCR Genotyping Cytology |
topic |
HPV Cervical cancer PCR Genotyping Cytology |
description |
PURPOSE: The aim of this study was to investigate the frequency of HPV infection and the types 16 and 18 in cervical samples from patients attended at two public health services of the city of Belo Horizonte, MG. METHODS: Cervical samples from 174 patients were collected for cytopathological and molecular tests. HPV infection was searched by PCR utilizing MY09 and MY11 primers or HPV 16 and HPV 18 specific primers. RESULTS: Amongst the 174 samples analyzed, 20.7% presented squamous intraepithelial and/or invasive lesions detected on cytopathological analysis and of those, 94.4% were infected by HPV. HPV 16 was found in 20% of the cases of low-grade squamous intraepithelial lesions and in 40% and 50% of high-grade squamous intraepithelial lesion and squamous invasive carcinoma, respectively. HPV 18 was detected in 6.7% of the low-grade lesion samples and in two HPV16 co-infected samples. In 50% of the cases of high-grade lesion, the HPV type was not determined. CONCLUSION: The HPV 16 was the virus type more frequently detected. However, more than 50% of the positive samples at the cytopathological analysis were negative for HPV 16 and 18, indicating that possibly other virus types are present in relative high frequencies in the studied population. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31114 |
url |
https://www.revistas.usp.br/rimtsp/article/view/31114 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31114/32998 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 No. 5 (2007); 297-301 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 Núm. 5 (2007); 297-301 Revista do Instituto de Medicina Tropical de São Paulo; v. 49 n. 5 (2007); 297-301 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
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