Evaluation of Trypanosoma cruzi antigens for the indirect hemagglutination test: II. Different stocks and evolutive forms

Detalhes bibliográficos
Autor(a) principal: Vítor, Ricardo Wagner de Almeida
Data de Publicação: 1987
Outros Autores: Chiari, Egler
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28515
Resumo: To improve sensitivity and specificity of the indirect hemagglutination test (IHA) for Chagas' disease serodiagnosis, different antigens of Trypanosoma cruzi extracts from epimastigotes (A1.7, B12 and CI clones; Y and D150 strains) and from trypomastigotes (metacyclics and tissue cell cultures) -were tested. Sera of 132 individuals with chronic Chagas' disease, demonstrated by immunofluorescence test (IFT) and sera of 161 non chagasic individuals were assayed. The IFT was the reference test to evaluate the co-positivity index (c.p.i.) and co-negativity index (c.ni.). The antigens of Y strain and B12 clone presented high values for the calculated index with a cut off of 1:40, suggesting sensitivity, specificity and agreement of IHA in relation to IFT. Antigens of A1.7 and CI clones presented high values for the calculated index only with a cut off of 1:80. Titers presented by sera from chagasics tested by IHA with trypomastigote antigens were lower than those with epimastigote antigens. Trypomastigote antigens gave lower values for co-positivity index, suggesting little sensitivity in relation to IFT.
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spelling Evaluation of Trypanosoma cruzi antigens for the indirect hemagglutination test: II. Different stocks and evolutive forms Avaliação de antígenos do trypanosoma cruzi para a reação de hemaglutinação indireta: II. Antígenos de diferentes amostras e formas evolutivas Doença de ChagasDiagósticoSorologiaHemaglutinação indireta To improve sensitivity and specificity of the indirect hemagglutination test (IHA) for Chagas' disease serodiagnosis, different antigens of Trypanosoma cruzi extracts from epimastigotes (A1.7, B12 and CI clones; Y and D150 strains) and from trypomastigotes (metacyclics and tissue cell cultures) -were tested. Sera of 132 individuals with chronic Chagas' disease, demonstrated by immunofluorescence test (IFT) and sera of 161 non chagasic individuals were assayed. The IFT was the reference test to evaluate the co-positivity index (c.p.i.) and co-negativity index (c.ni.). The antigens of Y strain and B12 clone presented high values for the calculated index with a cut off of 1:40, suggesting sensitivity, specificity and agreement of IHA in relation to IFT. Antigens of A1.7 and CI clones presented high values for the calculated index only with a cut off of 1:80. Titers presented by sera from chagasics tested by IHA with trypomastigote antigens were lower than those with epimastigote antigens. Trypomastigote antigens gave lower values for co-positivity index, suggesting little sensitivity in relation to IFT. Para aprimorar a sensibilidade e a especificidade da reação de hemaglutina-ção indireta no diagnóstico da doença de Chagas, foram utilizados eritrocitos sensibilizados com antígeno de epimastigotas de diferentes amostras (clones Al.7, B12, Cl e cepas D150 e Y), de tripomastigotas metacíclicos de cultura (TMC) e de tri-pomastigotas de cultura de células (TCC) do Trypanosoma cruzi. Foram titulados 132 soros de indivíduos chagásicos e 161 soros de não chagásicos, diagnosticados pela reação de imunofluorescência indireta (RIFI), considerada como reação de referência no cálculo dos índices de co-positividade (i.c.p.), co-negatividade (i.c.n.) e concordância. Utilizando o título discriminante (T.D.) de 1:40, os antígenos da cepa Y e do clone B12 apresentaram valores elevados para os três índices calculados, indicando boa sensibilidade e especificidade em relação à RIFI. Os antígenos dos clones Al .7 e Cl apresentaram baixa especificidade considerando o T.D. de 1:40, mas ocorreu um aumento acentuado para o i.c.n. considerando o T.D. de 1:80, indicando um aumento de especificidade relativa sem alteração significativa da sensibilidade relativa. Com os antígenos T.M.C, e T.C.C, foram observados títulos mais baixos e valores menores para o i.c.p., indicando pouca sensibilidade em relação à RIFI. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1987-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28515Revista do Instituto de Medicina Tropical de São Paulo; Vol. 29 No. 3 (1987); 183-188 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 29 Núm. 3 (1987); 183-188 Revista do Instituto de Medicina Tropical de São Paulo; v. 29 n. 3 (1987); 183-188 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/28515/30368Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessVítor, Ricardo Wagner de AlmeidaChiari, Egler2012-07-02T00:57:33Zoai:revistas.usp.br:article/28515Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:14.721691Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Evaluation of Trypanosoma cruzi antigens for the indirect hemagglutination test: II. Different stocks and evolutive forms
Avaliação de antígenos do trypanosoma cruzi para a reação de hemaglutinação indireta: II. Antígenos de diferentes amostras e formas evolutivas
title Evaluation of Trypanosoma cruzi antigens for the indirect hemagglutination test: II. Different stocks and evolutive forms
spellingShingle Evaluation of Trypanosoma cruzi antigens for the indirect hemagglutination test: II. Different stocks and evolutive forms
Vítor, Ricardo Wagner de Almeida
Doença de Chagas
Diagóstico
Sorologia
Hemaglutinação indireta
title_short Evaluation of Trypanosoma cruzi antigens for the indirect hemagglutination test: II. Different stocks and evolutive forms
title_full Evaluation of Trypanosoma cruzi antigens for the indirect hemagglutination test: II. Different stocks and evolutive forms
title_fullStr Evaluation of Trypanosoma cruzi antigens for the indirect hemagglutination test: II. Different stocks and evolutive forms
title_full_unstemmed Evaluation of Trypanosoma cruzi antigens for the indirect hemagglutination test: II. Different stocks and evolutive forms
title_sort Evaluation of Trypanosoma cruzi antigens for the indirect hemagglutination test: II. Different stocks and evolutive forms
author Vítor, Ricardo Wagner de Almeida
author_facet Vítor, Ricardo Wagner de Almeida
Chiari, Egler
author_role author
author2 Chiari, Egler
author2_role author
dc.contributor.author.fl_str_mv Vítor, Ricardo Wagner de Almeida
Chiari, Egler
dc.subject.por.fl_str_mv Doença de Chagas
Diagóstico
Sorologia
Hemaglutinação indireta
topic Doença de Chagas
Diagóstico
Sorologia
Hemaglutinação indireta
description To improve sensitivity and specificity of the indirect hemagglutination test (IHA) for Chagas' disease serodiagnosis, different antigens of Trypanosoma cruzi extracts from epimastigotes (A1.7, B12 and CI clones; Y and D150 strains) and from trypomastigotes (metacyclics and tissue cell cultures) -were tested. Sera of 132 individuals with chronic Chagas' disease, demonstrated by immunofluorescence test (IFT) and sera of 161 non chagasic individuals were assayed. The IFT was the reference test to evaluate the co-positivity index (c.p.i.) and co-negativity index (c.ni.). The antigens of Y strain and B12 clone presented high values for the calculated index with a cut off of 1:40, suggesting sensitivity, specificity and agreement of IHA in relation to IFT. Antigens of A1.7 and CI clones presented high values for the calculated index only with a cut off of 1:80. Titers presented by sera from chagasics tested by IHA with trypomastigote antigens were lower than those with epimastigote antigens. Trypomastigote antigens gave lower values for co-positivity index, suggesting little sensitivity in relation to IFT.
publishDate 1987
dc.date.none.fl_str_mv 1987-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28515
url https://www.revistas.usp.br/rimtsp/article/view/28515
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28515/30368
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 29 No. 3 (1987); 183-188
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 29 Núm. 3 (1987); 183-188
Revista do Instituto de Medicina Tropical de São Paulo; v. 29 n. 3 (1987); 183-188
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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