Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol

Detalhes bibliográficos
Autor(a) principal: Gea, Susana
Data de Publicação: 1992
Outros Autores: Gruppi, Adriana, Cerban, Fabio, Pistoresi Palencia, Maria C., Vottero-Cima, Elsa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28956
Resumo: The humoral and cellular immune responses as well as the resistance to infection with bloodstream forms of T. cruzi were studied in mice immunized with acidic antigenic fractions from parasite cytosol, F III and F IV, plus Bordetella pertussis as adjuvant. The immunization with F III induced positive ITH and DTH responses to homologous antigens. In mice immunized with F IV, the ITH was negative and four out of six animals presented positive DTH reactions. In both groups of mice the analysis of IgG aginst T. cruzi showed that the major isotype elicited was IgG1. Specific IgE was also detected in sera from F III immunized mice, thus confirming the presence of homocytothropic antibodies. The parasitemias reached by F III and F IV immunized mice after challenge were lower than those of the controls showing in this way a partial protection against the acute infection. The histological studies of heart and skeletal muscle performed two months after the infection revealed variable mononuclear infiltration in all infected mice despite immunization.
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spelling Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol Resposta imune em camundongos imunizados com a fração ácida antigênica do Citosol do Trypanosoma cruzi Trypanosoma cruziImmune responseAcidic antigens The humoral and cellular immune responses as well as the resistance to infection with bloodstream forms of T. cruzi were studied in mice immunized with acidic antigenic fractions from parasite cytosol, F III and F IV, plus Bordetella pertussis as adjuvant. The immunization with F III induced positive ITH and DTH responses to homologous antigens. In mice immunized with F IV, the ITH was negative and four out of six animals presented positive DTH reactions. In both groups of mice the analysis of IgG aginst T. cruzi showed that the major isotype elicited was IgG1. Specific IgE was also detected in sera from F III immunized mice, thus confirming the presence of homocytothropic antibodies. The parasitemias reached by F III and F IV immunized mice after challenge were lower than those of the controls showing in this way a partial protection against the acute infection. The histological studies of heart and skeletal muscle performed two months after the infection revealed variable mononuclear infiltration in all infected mice despite immunization. As respostas humorais e celulares, assim como a resistência à infecção a formas sanguícolas do T. cruzi foram estudadas em camundongos imunizados com as frações antigênicas ácidas do Citosol parasitário, F III e F IV com Bordetella pertussis como adjuvante. A imunização com F III induziu respostas positivas ITH e DTH aos antígenos homólogos. Em camundongos imunizados com F IV o ITH foi negativo e quatro dos seis animais apresentaram reações DTH positivas. Em ambos os grupos de camundongos a análise de IgG contra T. cruzi mostrou que o principal isotipo produzido foi IgG1. IgE específico foi também detectado em soros de camundongos imunizados com F III, portanto confirmando a presença de anticorpos homocitotrópicos. As parasitemias atingidas pelos camundongos imunizados por F III e F IV após desafio foram mais baixas do que aquelas dos controles mostrando proteção parcial contra a infecção aguda. Os estudos histológicos do coração e do músculo esquelético realizados dois meses depois da infecção revelaram infiltrado mononuclear de intensidade variável em todos os camundongos infectados a despeito da imunização. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1992-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28956Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 5 (1992); 389-394 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 5 (1992); 389-394 Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 5 (1992); 389-394 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28956/30813Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessGea, SusanaGruppi, AdrianaCerban, FabioPistoresi Palencia, Maria C.Vottero-Cima, Elsa2012-07-02T01:33:31Zoai:revistas.usp.br:article/28956Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:43.226417Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol
Resposta imune em camundongos imunizados com a fração ácida antigênica do Citosol do Trypanosoma cruzi
title Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol
spellingShingle Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol
Gea, Susana
Trypanosoma cruzi
Immune response
Acidic antigens
title_short Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol
title_full Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol
title_fullStr Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol
title_full_unstemmed Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol
title_sort Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol
author Gea, Susana
author_facet Gea, Susana
Gruppi, Adriana
Cerban, Fabio
Pistoresi Palencia, Maria C.
Vottero-Cima, Elsa
author_role author
author2 Gruppi, Adriana
Cerban, Fabio
Pistoresi Palencia, Maria C.
Vottero-Cima, Elsa
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gea, Susana
Gruppi, Adriana
Cerban, Fabio
Pistoresi Palencia, Maria C.
Vottero-Cima, Elsa
dc.subject.por.fl_str_mv Trypanosoma cruzi
Immune response
Acidic antigens
topic Trypanosoma cruzi
Immune response
Acidic antigens
description The humoral and cellular immune responses as well as the resistance to infection with bloodstream forms of T. cruzi were studied in mice immunized with acidic antigenic fractions from parasite cytosol, F III and F IV, plus Bordetella pertussis as adjuvant. The immunization with F III induced positive ITH and DTH responses to homologous antigens. In mice immunized with F IV, the ITH was negative and four out of six animals presented positive DTH reactions. In both groups of mice the analysis of IgG aginst T. cruzi showed that the major isotype elicited was IgG1. Specific IgE was also detected in sera from F III immunized mice, thus confirming the presence of homocytothropic antibodies. The parasitemias reached by F III and F IV immunized mice after challenge were lower than those of the controls showing in this way a partial protection against the acute infection. The histological studies of heart and skeletal muscle performed two months after the infection revealed variable mononuclear infiltration in all infected mice despite immunization.
publishDate 1992
dc.date.none.fl_str_mv 1992-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28956
url https://www.revistas.usp.br/rimtsp/article/view/28956
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28956/30813
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 5 (1992); 389-394
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 5 (1992); 389-394
Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 5 (1992); 389-394
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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