Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol
Autor(a) principal: | |
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Data de Publicação: | 1992 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28956 |
Resumo: | The humoral and cellular immune responses as well as the resistance to infection with bloodstream forms of T. cruzi were studied in mice immunized with acidic antigenic fractions from parasite cytosol, F III and F IV, plus Bordetella pertussis as adjuvant. The immunization with F III induced positive ITH and DTH responses to homologous antigens. In mice immunized with F IV, the ITH was negative and four out of six animals presented positive DTH reactions. In both groups of mice the analysis of IgG aginst T. cruzi showed that the major isotype elicited was IgG1. Specific IgE was also detected in sera from F III immunized mice, thus confirming the presence of homocytothropic antibodies. The parasitemias reached by F III and F IV immunized mice after challenge were lower than those of the controls showing in this way a partial protection against the acute infection. The histological studies of heart and skeletal muscle performed two months after the infection revealed variable mononuclear infiltration in all infected mice despite immunization. |
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Revista do Instituto de Medicina Tropical de São Paulo |
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Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol Resposta imune em camundongos imunizados com a fração ácida antigênica do Citosol do Trypanosoma cruzi Trypanosoma cruziImmune responseAcidic antigens The humoral and cellular immune responses as well as the resistance to infection with bloodstream forms of T. cruzi were studied in mice immunized with acidic antigenic fractions from parasite cytosol, F III and F IV, plus Bordetella pertussis as adjuvant. The immunization with F III induced positive ITH and DTH responses to homologous antigens. In mice immunized with F IV, the ITH was negative and four out of six animals presented positive DTH reactions. In both groups of mice the analysis of IgG aginst T. cruzi showed that the major isotype elicited was IgG1. Specific IgE was also detected in sera from F III immunized mice, thus confirming the presence of homocytothropic antibodies. The parasitemias reached by F III and F IV immunized mice after challenge were lower than those of the controls showing in this way a partial protection against the acute infection. The histological studies of heart and skeletal muscle performed two months after the infection revealed variable mononuclear infiltration in all infected mice despite immunization. As respostas humorais e celulares, assim como a resistência à infecção a formas sanguícolas do T. cruzi foram estudadas em camundongos imunizados com as frações antigênicas ácidas do Citosol parasitário, F III e F IV com Bordetella pertussis como adjuvante. A imunização com F III induziu respostas positivas ITH e DTH aos antígenos homólogos. Em camundongos imunizados com F IV o ITH foi negativo e quatro dos seis animais apresentaram reações DTH positivas. Em ambos os grupos de camundongos a análise de IgG contra T. cruzi mostrou que o principal isotipo produzido foi IgG1. IgE específico foi também detectado em soros de camundongos imunizados com F III, portanto confirmando a presença de anticorpos homocitotrópicos. As parasitemias atingidas pelos camundongos imunizados por F III e F IV após desafio foram mais baixas do que aquelas dos controles mostrando proteção parcial contra a infecção aguda. Os estudos histológicos do coração e do músculo esquelético realizados dois meses depois da infecção revelaram infiltrado mononuclear de intensidade variável em todos os camundongos infectados a despeito da imunização. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1992-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28956Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 5 (1992); 389-394 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 5 (1992); 389-394 Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 5 (1992); 389-394 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28956/30813Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessGea, SusanaGruppi, AdrianaCerban, FabioPistoresi Palencia, Maria C.Vottero-Cima, Elsa2012-07-02T01:33:31Zoai:revistas.usp.br:article/28956Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:43.226417Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol Resposta imune em camundongos imunizados com a fração ácida antigênica do Citosol do Trypanosoma cruzi |
title |
Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol |
spellingShingle |
Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol Gea, Susana Trypanosoma cruzi Immune response Acidic antigens |
title_short |
Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol |
title_full |
Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol |
title_fullStr |
Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol |
title_full_unstemmed |
Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol |
title_sort |
Immune response in mice immunized with acidic antigenic fractions from Trypanosoma cruzi cytosol |
author |
Gea, Susana |
author_facet |
Gea, Susana Gruppi, Adriana Cerban, Fabio Pistoresi Palencia, Maria C. Vottero-Cima, Elsa |
author_role |
author |
author2 |
Gruppi, Adriana Cerban, Fabio Pistoresi Palencia, Maria C. Vottero-Cima, Elsa |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Gea, Susana Gruppi, Adriana Cerban, Fabio Pistoresi Palencia, Maria C. Vottero-Cima, Elsa |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi Immune response Acidic antigens |
topic |
Trypanosoma cruzi Immune response Acidic antigens |
description |
The humoral and cellular immune responses as well as the resistance to infection with bloodstream forms of T. cruzi were studied in mice immunized with acidic antigenic fractions from parasite cytosol, F III and F IV, plus Bordetella pertussis as adjuvant. The immunization with F III induced positive ITH and DTH responses to homologous antigens. In mice immunized with F IV, the ITH was negative and four out of six animals presented positive DTH reactions. In both groups of mice the analysis of IgG aginst T. cruzi showed that the major isotype elicited was IgG1. Specific IgE was also detected in sera from F III immunized mice, thus confirming the presence of homocytothropic antibodies. The parasitemias reached by F III and F IV immunized mice after challenge were lower than those of the controls showing in this way a partial protection against the acute infection. The histological studies of heart and skeletal muscle performed two months after the infection revealed variable mononuclear infiltration in all infected mice despite immunization. |
publishDate |
1992 |
dc.date.none.fl_str_mv |
1992-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28956 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28956 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28956/30813 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 5 (1992); 389-394 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 5 (1992); 389-394 Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 5 (1992); 389-394 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951639887904768 |