Radiometric studies on the oxidation of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria
Autor(a) principal: | |
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Data de Publicação: | 1987 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28536 |
Resumo: | A radiometric assay system has been used to study oxidation patterns of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria. Drug-susceptible M. tuberculosis (H37Rv TMC 102 and Erdman) along with the drug-resistant organism M. tuberculosis (H37 Rv TMC 303), M. bovis, M. avium, M. intracellulare, M. kansasii and M. chelonei were used. The organisms were inoculated into a sterile reaction system with liquid 7H9 medium and one of the (U-14C) L-amino acids. Each organism displayed a different pattern of amino acid oxidation, but these patterns were not distinctive enough for identification of the organism. Complex amino acids such as proline, phenylalanine and tyrosine were of no use in identification of mycobacteria, since virtually all organisms failed to oxidize them. There was no combination of substrates able to separate susceptible from resistant organisms. |
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Radiometric studies on the oxidation of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria Estudos radiométricos sobre a oxidação de (U-14C) L-aminoácidos por micobactérias sensíveis e resistentes a drogas Radiometric assayM. tuberculosis(U-14C) L-Amino AcidsDrug resistance A radiometric assay system has been used to study oxidation patterns of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria. Drug-susceptible M. tuberculosis (H37Rv TMC 102 and Erdman) along with the drug-resistant organism M. tuberculosis (H37 Rv TMC 303), M. bovis, M. avium, M. intracellulare, M. kansasii and M. chelonei were used. The organisms were inoculated into a sterile reaction system with liquid 7H9 medium and one of the (U-14C) L-amino acids. Each organism displayed a different pattern of amino acid oxidation, but these patterns were not distinctive enough for identification of the organism. Complex amino acids such as proline, phenylalanine and tyrosine were of no use in identification of mycobacteria, since virtually all organisms failed to oxidize them. There was no combination of substrates able to separate susceptible from resistant organisms. Um sistema radiométrico foi utilizado para estudar os padrões de oxidação dos (U-14C) L-aminoácidos por micobactérias sensíveis e resis tentes a drogas. Foram usadas duas cepas do M. tuberculosis sensíveis a todas as drogas, H37Rv e Erdman. As micobactérias resistentes foram M. tuberculosis H37Rv resistente a 5 ug/ml de hidrazida, M. bovis, M. avium, M. intracellulare, M. kansasii e M. chelonei. As micobac térias foram inoculadas em frascos estéreis contendo o meio líquido 7H9 e um dos (U-14C) L-aminoácidos. Cada micobactéria apresentou um padrão de oxidação de aminoácidos, mas estes padrões não foram suficientemente diferentes para identificá-la. Aminoácidos complexos como a prolina, fenilalanina e tirosina não tiveram utilidade na identificação das micobactérias, pois praticamente todos os microorganismos foram incapazes de oxidá-los. Nenhuma combinação de aminoácidos foi capaz de separar as micobactérias sensíveis das resistentes a drogas. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1987-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28536Revista do Instituto de Medicina Tropical de São Paulo; Vol. 29 No. 5 (1987); 312-316 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 29 Núm. 5 (1987); 312-316 Revista do Instituto de Medicina Tropical de São Paulo; v. 29 n. 5 (1987); 312-316 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28536/30389Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCamargo, Edwaldo E.Kopajtic, Teresa M.Hopkins, Glinda K.Cannon, Nancy P.Wagner Jr, Henry N.2012-07-02T00:58:46Zoai:revistas.usp.br:article/28536Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:16.142213Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Radiometric studies on the oxidation of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria Estudos radiométricos sobre a oxidação de (U-14C) L-aminoácidos por micobactérias sensíveis e resistentes a drogas |
title |
Radiometric studies on the oxidation of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria |
spellingShingle |
Radiometric studies on the oxidation of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria Camargo, Edwaldo E. Radiometric assay M. tuberculosis (U-14C) L-Amino Acids Drug resistance |
title_short |
Radiometric studies on the oxidation of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria |
title_full |
Radiometric studies on the oxidation of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria |
title_fullStr |
Radiometric studies on the oxidation of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria |
title_full_unstemmed |
Radiometric studies on the oxidation of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria |
title_sort |
Radiometric studies on the oxidation of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria |
author |
Camargo, Edwaldo E. |
author_facet |
Camargo, Edwaldo E. Kopajtic, Teresa M. Hopkins, Glinda K. Cannon, Nancy P. Wagner Jr, Henry N. |
author_role |
author |
author2 |
Kopajtic, Teresa M. Hopkins, Glinda K. Cannon, Nancy P. Wagner Jr, Henry N. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Camargo, Edwaldo E. Kopajtic, Teresa M. Hopkins, Glinda K. Cannon, Nancy P. Wagner Jr, Henry N. |
dc.subject.por.fl_str_mv |
Radiometric assay M. tuberculosis (U-14C) L-Amino Acids Drug resistance |
topic |
Radiometric assay M. tuberculosis (U-14C) L-Amino Acids Drug resistance |
description |
A radiometric assay system has been used to study oxidation patterns of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria. Drug-susceptible M. tuberculosis (H37Rv TMC 102 and Erdman) along with the drug-resistant organism M. tuberculosis (H37 Rv TMC 303), M. bovis, M. avium, M. intracellulare, M. kansasii and M. chelonei were used. The organisms were inoculated into a sterile reaction system with liquid 7H9 medium and one of the (U-14C) L-amino acids. Each organism displayed a different pattern of amino acid oxidation, but these patterns were not distinctive enough for identification of the organism. Complex amino acids such as proline, phenylalanine and tyrosine were of no use in identification of mycobacteria, since virtually all organisms failed to oxidize them. There was no combination of substrates able to separate susceptible from resistant organisms. |
publishDate |
1987 |
dc.date.none.fl_str_mv |
1987-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28536 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28536 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28536/30389 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 29 No. 5 (1987); 312-316 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 29 Núm. 5 (1987); 312-316 Revista do Instituto de Medicina Tropical de São Paulo; v. 29 n. 5 (1987); 312-316 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951637230813184 |