PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE

Detalhes bibliográficos
Autor(a) principal: Avila, Luciana Farias da Costa de
Data de Publicação: 2013
Outros Autores: Telmo, Paula de Lima, Martins, Lourdes Helena Rodrigues, Glaeser, Thais Aimee, Conceicao, Fabricio Rochedo, Leite, Fabio Pereira Leivas, Scaini, Carlos James
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/78672
Resumo: SUMMARY In a previous study our group found that the probiotic Saccharomyces boulardii was capable of reducing the intensity of infection in mice with toxocariasis. In order to assess whether the mechanism involved would be a direct action of the probiotic on Toxocara canis larvae, this study was designed. Both probiotics were singly cultivated in plates containing RPMI 1640 medium and T. canis larvae. S. boulardii and B. cereus var. toyoi cultures presented 97.6% and 95.7% of larvae with positive motility, respectively, and absence of color by the dye trypan blue, not representing significant difference to the control group (p >; 0.05). We conclude that none of the probiotics showed in vitro effects on T. canis larvae and that the interaction with the intestinal mucosa is necessary for the development of the protective effect of S. boulardii.
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spelling PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE Efeito protetor do probiótico Saccharomyces boulardii na infecção por Toxocara canis não se deve à ação direta sobre as larvas SUMMARY In a previous study our group found that the probiotic Saccharomyces boulardii was capable of reducing the intensity of infection in mice with toxocariasis. In order to assess whether the mechanism involved would be a direct action of the probiotic on Toxocara canis larvae, this study was designed. Both probiotics were singly cultivated in plates containing RPMI 1640 medium and T. canis larvae. S. boulardii and B. cereus var. toyoi cultures presented 97.6% and 95.7% of larvae with positive motility, respectively, and absence of color by the dye trypan blue, not representing significant difference to the control group (p >; 0.05). We conclude that none of the probiotics showed in vitro effects on T. canis larvae and that the interaction with the intestinal mucosa is necessary for the development of the protective effect of S. boulardii. RESUMO Em estudo prévio nosso grupo verificou que o probiótico Saccharomyces boulardii foi capaz de reduzir a intensidade de infecção em camundongos com toxocaríase. Este estudo foi elaborado com o objetivo de avaliar se o mecanismo envolvido seria uma ação direta do probiótico sobre as larvas de Toxocara canis. Ambos probióticos foram cultivados, separadamente, em placas com meio RPMI1640 e larvas de T. canis. As culturas com S. boulardii e B. cereus var. toyoi apresentaram, respectivamente, 97,6% e 95,7% das larvas com motilidade positiva e ausência de coloração pelo azul de tripan, não representando diferença significativa do controle (p >; 0,05). Concluímos que nenhum dos probióticos apresentou efeito in vitro sobre as larvas de T. canis e que a interação com a mucosa intestinal é necessária para o desenvolvimento do efeito protetor de S. boulardii. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/78672Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 No. 5 (2013); 363-365Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 Núm. 5 (2013); 363-365Revista do Instituto de Medicina Tropical de São Paulo; v. 55 n. 5 (2013); 363-3651678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/78672/82725Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessAvila, Luciana Farias da Costa de Telmo, Paula de Lima Martins, Lourdes Helena Rodrigues Glaeser, Thais Aimee Conceicao, Fabricio Rochedo Leite, Fabio Pereira Leivas Scaini, Carlos James 2014-04-03T17:11:06Zoai:revistas.usp.br:article/78672Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:14.769Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE
Efeito protetor do probiótico Saccharomyces boulardii na infecção por Toxocara canis não se deve à ação direta sobre as larvas
title PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE
spellingShingle PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE
Avila, Luciana Farias da Costa de
title_short PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE
title_full PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE
title_fullStr PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE
title_full_unstemmed PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE
title_sort PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE
author Avila, Luciana Farias da Costa de
author_facet Avila, Luciana Farias da Costa de
Telmo, Paula de Lima
Martins, Lourdes Helena Rodrigues
Glaeser, Thais Aimee
Conceicao, Fabricio Rochedo
Leite, Fabio Pereira Leivas
Scaini, Carlos James
author_role author
author2 Telmo, Paula de Lima
Martins, Lourdes Helena Rodrigues
Glaeser, Thais Aimee
Conceicao, Fabricio Rochedo
Leite, Fabio Pereira Leivas
Scaini, Carlos James
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Avila, Luciana Farias da Costa de
Telmo, Paula de Lima
Martins, Lourdes Helena Rodrigues
Glaeser, Thais Aimee
Conceicao, Fabricio Rochedo
Leite, Fabio Pereira Leivas
Scaini, Carlos James
description SUMMARY In a previous study our group found that the probiotic Saccharomyces boulardii was capable of reducing the intensity of infection in mice with toxocariasis. In order to assess whether the mechanism involved would be a direct action of the probiotic on Toxocara canis larvae, this study was designed. Both probiotics were singly cultivated in plates containing RPMI 1640 medium and T. canis larvae. S. boulardii and B. cereus var. toyoi cultures presented 97.6% and 95.7% of larvae with positive motility, respectively, and absence of color by the dye trypan blue, not representing significant difference to the control group (p >; 0.05). We conclude that none of the probiotics showed in vitro effects on T. canis larvae and that the interaction with the intestinal mucosa is necessary for the development of the protective effect of S. boulardii.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/78672
url https://www.revistas.usp.br/rimtsp/article/view/78672
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/78672/82725
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 No. 5 (2013); 363-365
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 Núm. 5 (2013); 363-365
Revista do Instituto de Medicina Tropical de São Paulo; v. 55 n. 5 (2013); 363-365
1678-9946
0036-4665
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repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
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