PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/78672 |
Resumo: | SUMMARY In a previous study our group found that the probiotic Saccharomyces boulardii was capable of reducing the intensity of infection in mice with toxocariasis. In order to assess whether the mechanism involved would be a direct action of the probiotic on Toxocara canis larvae, this study was designed. Both probiotics were singly cultivated in plates containing RPMI 1640 medium and T. canis larvae. S. boulardii and B. cereus var. toyoi cultures presented 97.6% and 95.7% of larvae with positive motility, respectively, and absence of color by the dye trypan blue, not representing significant difference to the control group (p >; 0.05). We conclude that none of the probiotics showed in vitro effects on T. canis larvae and that the interaction with the intestinal mucosa is necessary for the development of the protective effect of S. boulardii. |
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Revista do Instituto de Medicina Tropical de São Paulo |
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PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE Efeito protetor do probiótico Saccharomyces boulardii na infecção por Toxocara canis não se deve à ação direta sobre as larvas SUMMARY In a previous study our group found that the probiotic Saccharomyces boulardii was capable of reducing the intensity of infection in mice with toxocariasis. In order to assess whether the mechanism involved would be a direct action of the probiotic on Toxocara canis larvae, this study was designed. Both probiotics were singly cultivated in plates containing RPMI 1640 medium and T. canis larvae. S. boulardii and B. cereus var. toyoi cultures presented 97.6% and 95.7% of larvae with positive motility, respectively, and absence of color by the dye trypan blue, not representing significant difference to the control group (p >; 0.05). We conclude that none of the probiotics showed in vitro effects on T. canis larvae and that the interaction with the intestinal mucosa is necessary for the development of the protective effect of S. boulardii. RESUMO Em estudo prévio nosso grupo verificou que o probiótico Saccharomyces boulardii foi capaz de reduzir a intensidade de infecção em camundongos com toxocaríase. Este estudo foi elaborado com o objetivo de avaliar se o mecanismo envolvido seria uma ação direta do probiótico sobre as larvas de Toxocara canis. Ambos probióticos foram cultivados, separadamente, em placas com meio RPMI1640 e larvas de T. canis. As culturas com S. boulardii e B. cereus var. toyoi apresentaram, respectivamente, 97,6% e 95,7% das larvas com motilidade positiva e ausência de coloração pelo azul de tripan, não representando diferença significativa do controle (p >; 0,05). Concluímos que nenhum dos probióticos apresentou efeito in vitro sobre as larvas de T. canis e que a interação com a mucosa intestinal é necessária para o desenvolvimento do efeito protetor de S. boulardii. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/78672Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 No. 5 (2013); 363-365Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 Núm. 5 (2013); 363-365Revista do Instituto de Medicina Tropical de São Paulo; v. 55 n. 5 (2013); 363-3651678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/78672/82725Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessAvila, Luciana Farias da Costa de Telmo, Paula de Lima Martins, Lourdes Helena Rodrigues Glaeser, Thais Aimee Conceicao, Fabricio Rochedo Leite, Fabio Pereira Leivas Scaini, Carlos James 2014-04-03T17:11:06Zoai:revistas.usp.br:article/78672Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:14.769Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE Efeito protetor do probiótico Saccharomyces boulardii na infecção por Toxocara canis não se deve à ação direta sobre as larvas |
title |
PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE |
spellingShingle |
PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE Avila, Luciana Farias da Costa de |
title_short |
PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE |
title_full |
PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE |
title_fullStr |
PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE |
title_full_unstemmed |
PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE |
title_sort |
PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE |
author |
Avila, Luciana Farias da Costa de |
author_facet |
Avila, Luciana Farias da Costa de Telmo, Paula de Lima Martins, Lourdes Helena Rodrigues Glaeser, Thais Aimee Conceicao, Fabricio Rochedo Leite, Fabio Pereira Leivas Scaini, Carlos James |
author_role |
author |
author2 |
Telmo, Paula de Lima Martins, Lourdes Helena Rodrigues Glaeser, Thais Aimee Conceicao, Fabricio Rochedo Leite, Fabio Pereira Leivas Scaini, Carlos James |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Avila, Luciana Farias da Costa de Telmo, Paula de Lima Martins, Lourdes Helena Rodrigues Glaeser, Thais Aimee Conceicao, Fabricio Rochedo Leite, Fabio Pereira Leivas Scaini, Carlos James |
description |
SUMMARY In a previous study our group found that the probiotic Saccharomyces boulardii was capable of reducing the intensity of infection in mice with toxocariasis. In order to assess whether the mechanism involved would be a direct action of the probiotic on Toxocara canis larvae, this study was designed. Both probiotics were singly cultivated in plates containing RPMI 1640 medium and T. canis larvae. S. boulardii and B. cereus var. toyoi cultures presented 97.6% and 95.7% of larvae with positive motility, respectively, and absence of color by the dye trypan blue, not representing significant difference to the control group (p >; 0.05). We conclude that none of the probiotics showed in vitro effects on T. canis larvae and that the interaction with the intestinal mucosa is necessary for the development of the protective effect of S. boulardii. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/78672 |
url |
https://www.revistas.usp.br/rimtsp/article/view/78672 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/78672/82725 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 No. 5 (2013); 363-365 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 55 Núm. 5 (2013); 363-365 Revista do Instituto de Medicina Tropical de São Paulo; v. 55 n. 5 (2013); 363-365 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951648990593024 |