Histopathology, serology and cultures in the diagnosis of cryptococcosis

Detalhes bibliográficos
Autor(a) principal: Gazzoni, Alexandra Flávia
Data de Publicação: 2009
Outros Autores: Severo, Cecília Bittencourt, Salles, Emily Ferreira, Severo, Luiz Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31285
Resumo: Cryptococcosis is one of the most common opportunistic fungal infections in patients with acquired immunodeficiency syndrome (AIDS). We report 13 cases of cryptococcal infection based on histopathology, serology and cultures. Epidemiological analysis, histochemical techniques of hematoxilin and eosin (HE) and Grocot's silver (GMS), as well special histochemical techniques such as Mayer's mucicarmine (MM) and Fontana-Masson (FM), cryptococcal antigen test (CrAg) and isolation on fungal media: Sabouraud's (SAB), brain-heart infusion agar (BHI) and canavanine-glycine-bromothymol blue (CGB) agar were analyzed. Unsatisfactory staining results by MM stain associated to negative titers by CrAg test, which FM stain confirmed that capsule-deficient Cryptococcus infections were observed in four cases. Eight isolated cases were identified as follows: six cases were infection with Cryptococcus neoformans and two cases were Cryptococcus gattii.
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spelling Histopathology, serology and cultures in the diagnosis of cryptococcosis Histopatologia, sorologia e cultivo no diagnóstico da criptococose Budding indexCarminophilic indexMayer's mucicarmine stainFontana-Masson stainCryptococcal antigen testCultures Cryptococcosis is one of the most common opportunistic fungal infections in patients with acquired immunodeficiency syndrome (AIDS). We report 13 cases of cryptococcal infection based on histopathology, serology and cultures. Epidemiological analysis, histochemical techniques of hematoxilin and eosin (HE) and Grocot's silver (GMS), as well special histochemical techniques such as Mayer's mucicarmine (MM) and Fontana-Masson (FM), cryptococcal antigen test (CrAg) and isolation on fungal media: Sabouraud's (SAB), brain-heart infusion agar (BHI) and canavanine-glycine-bromothymol blue (CGB) agar were analyzed. Unsatisfactory staining results by MM stain associated to negative titers by CrAg test, which FM stain confirmed that capsule-deficient Cryptococcus infections were observed in four cases. Eight isolated cases were identified as follows: six cases were infection with Cryptococcus neoformans and two cases were Cryptococcus gattii. A criptococose é a mais comum infecção fúngica oportunística observada em pacientes com síndrome da imunodeficiência adquirida (AIDS). Relatamos 13 casos da infecção baseados no diagnóstico histopatológico, sorológico e cultivo. Foram analisadas: a epidemiologia, as técnicas histoquímicas básicas de hematoxilina-eosina (HE) e coloração pela prata (GMS), bem como as técnicas histoquímicas especiais de mucicarmim de Mayer (MM) e Fontana-Masson (FM), o teste do antígeno criptocóccico (CrAg) e o isolamento em cultivos em ágar-Sabouraud (SAB), ágar infusão de cérebro-coração (BHI) e meio com canavanina azul de bromotimol (CGB). Em quatro casos, resultados tintoriais insatisfatórios pela coloração de MM associados a títulos negativos pelo teste do CrAg, a coloração de FM confirmou a infecção pelo Cryptococcus deficiente de cápsula. Oito isolados foram identificados: seis casos apresentaram a infecção por Cryptococcus neoformans e dois casos apresentaram a infecção por Cryptococcus gattii. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31285Revista do Instituto de Medicina Tropical de São Paulo; Vol. 51 No. 5 (2009); 255-259 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 51 Núm. 5 (2009); 255-259 Revista do Instituto de Medicina Tropical de São Paulo; v. 51 n. 5 (2009); 255-259 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31285/33170Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessGazzoni, Alexandra FláviaSevero, Cecília BittencourtSalles, Emily FerreiraSevero, Luiz Carlos2012-07-07T19:24:23Zoai:revistas.usp.br:article/31285Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:57.828282Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Histopathology, serology and cultures in the diagnosis of cryptococcosis
Histopatologia, sorologia e cultivo no diagnóstico da criptococose
title Histopathology, serology and cultures in the diagnosis of cryptococcosis
spellingShingle Histopathology, serology and cultures in the diagnosis of cryptococcosis
Gazzoni, Alexandra Flávia
Budding index
Carminophilic index
Mayer's mucicarmine stain
Fontana-Masson stain
Cryptococcal antigen test
Cultures
title_short Histopathology, serology and cultures in the diagnosis of cryptococcosis
title_full Histopathology, serology and cultures in the diagnosis of cryptococcosis
title_fullStr Histopathology, serology and cultures in the diagnosis of cryptococcosis
title_full_unstemmed Histopathology, serology and cultures in the diagnosis of cryptococcosis
title_sort Histopathology, serology and cultures in the diagnosis of cryptococcosis
author Gazzoni, Alexandra Flávia
author_facet Gazzoni, Alexandra Flávia
Severo, Cecília Bittencourt
Salles, Emily Ferreira
Severo, Luiz Carlos
author_role author
author2 Severo, Cecília Bittencourt
Salles, Emily Ferreira
Severo, Luiz Carlos
author2_role author
author
author
dc.contributor.author.fl_str_mv Gazzoni, Alexandra Flávia
Severo, Cecília Bittencourt
Salles, Emily Ferreira
Severo, Luiz Carlos
dc.subject.por.fl_str_mv Budding index
Carminophilic index
Mayer's mucicarmine stain
Fontana-Masson stain
Cryptococcal antigen test
Cultures
topic Budding index
Carminophilic index
Mayer's mucicarmine stain
Fontana-Masson stain
Cryptococcal antigen test
Cultures
description Cryptococcosis is one of the most common opportunistic fungal infections in patients with acquired immunodeficiency syndrome (AIDS). We report 13 cases of cryptococcal infection based on histopathology, serology and cultures. Epidemiological analysis, histochemical techniques of hematoxilin and eosin (HE) and Grocot's silver (GMS), as well special histochemical techniques such as Mayer's mucicarmine (MM) and Fontana-Masson (FM), cryptococcal antigen test (CrAg) and isolation on fungal media: Sabouraud's (SAB), brain-heart infusion agar (BHI) and canavanine-glycine-bromothymol blue (CGB) agar were analyzed. Unsatisfactory staining results by MM stain associated to negative titers by CrAg test, which FM stain confirmed that capsule-deficient Cryptococcus infections were observed in four cases. Eight isolated cases were identified as follows: six cases were infection with Cryptococcus neoformans and two cases were Cryptococcus gattii.
publishDate 2009
dc.date.none.fl_str_mv 2009-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31285
url https://www.revistas.usp.br/rimtsp/article/view/31285
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31285/33170
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 51 No. 5 (2009); 255-259
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 51 Núm. 5 (2009); 255-259
Revista do Instituto de Medicina Tropical de São Paulo; v. 51 n. 5 (2009); 255-259
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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