Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples

Detalhes bibliográficos
Autor(a) principal: Laferte, Jose
Data de Publicação: 1992
Outros Autores: Marrero, Miguel, Alvarez, Maritza, Jomarron, Liliana, Garcia, Serafina, Vazquez, Susana, Morier, Luis, Ulacia, Mario, Melchor, Antonio
Tipo de documento: Artigo
Idioma: spa
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28890
Resumo: We have standardized an indirect ultramicro ELISA assay for detecting antibodies to human Cytomegalovirus (CMV) using human serum samples (UMELISA CMV). The optimal concentration of coating antigen (30 ug/ml), serum dilution (1:40) and anti-human conjugate working dilution (1:1500), were determined by a check board titration method. The UMELISA CMV was compared with the latex agglutination test for antibodies to CMV (Dupont de Nemours) and with an indiret immunofluorescent method. The results have showed the high coincidence, sensitivity and especificity of the proposed assay regarding the two methods compared with, and supporting its use either for a blood donors screening or in the serological diagnosis of this infection by paired serum samples.
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spelling Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples UltramicroELISA indirecto para la deteccion de anticuerpos totales a citomegalovirus en suero humano UltramicroELISACitomegalovirus We have standardized an indirect ultramicro ELISA assay for detecting antibodies to human Cytomegalovirus (CMV) using human serum samples (UMELISA CMV). The optimal concentration of coating antigen (30 ug/ml), serum dilution (1:40) and anti-human conjugate working dilution (1:1500), were determined by a check board titration method. The UMELISA CMV was compared with the latex agglutination test for antibodies to CMV (Dupont de Nemours) and with an indiret immunofluorescent method. The results have showed the high coincidence, sensitivity and especificity of the proposed assay regarding the two methods compared with, and supporting its use either for a blood donors screening or in the serological diagnosis of this infection by paired serum samples. Se normalizó un ultramicroELISA indirecto para la detección de anticuerpos a Citomegalovirus (CMV) humano (UMELISA CMV). Se determinó la concentración óptima de antígeno en 30 ug/ml, la dilución de los sueros fue de 1:40 y la dilución de trabajo del conjugado fue de 1:1500. El UMELISA CMV fue comparado con las técnicas de aglutinación de latex para anticuerpos anti-CMV (Dupont de Neumors) y la inmunofluorescencia indirecta (EFT). Los resultados mostraron un alto grado de concordancia y elevada copositividad y conegatividad del UMELISA con respecto a estos dos ensayos. El método es válido para el pesquisaje de anticuerpos en banco de sangre asi como para el diagnóstico de la infección mediante sueros pareados. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1992-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28890Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 1 (1992); 43-47 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 1 (1992); 43-47 Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 1 (1992); 43-47 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTspahttps://www.revistas.usp.br/rimtsp/article/view/28890/30744Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessLaferte, JoseMarrero, MiguelAlvarez, MaritzaJomarron, LilianaGarcia, SerafinaVazquez, SusanaMorier, LuisUlacia, MarioMelchor, Antonio2012-07-02T01:32:25Zoai:revistas.usp.br:article/28890Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:39.297557Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples
UltramicroELISA indirecto para la deteccion de anticuerpos totales a citomegalovirus en suero humano
title Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples
spellingShingle Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples
Laferte, Jose
UltramicroELISA
Citomegalovirus
title_short Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples
title_full Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples
title_fullStr Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples
title_full_unstemmed Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples
title_sort Indirect Ultramicroelisa assay for the detection of human antibodies to cytomegalovirus using human serum samples
author Laferte, Jose
author_facet Laferte, Jose
Marrero, Miguel
Alvarez, Maritza
Jomarron, Liliana
Garcia, Serafina
Vazquez, Susana
Morier, Luis
Ulacia, Mario
Melchor, Antonio
author_role author
author2 Marrero, Miguel
Alvarez, Maritza
Jomarron, Liliana
Garcia, Serafina
Vazquez, Susana
Morier, Luis
Ulacia, Mario
Melchor, Antonio
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Laferte, Jose
Marrero, Miguel
Alvarez, Maritza
Jomarron, Liliana
Garcia, Serafina
Vazquez, Susana
Morier, Luis
Ulacia, Mario
Melchor, Antonio
dc.subject.por.fl_str_mv UltramicroELISA
Citomegalovirus
topic UltramicroELISA
Citomegalovirus
description We have standardized an indirect ultramicro ELISA assay for detecting antibodies to human Cytomegalovirus (CMV) using human serum samples (UMELISA CMV). The optimal concentration of coating antigen (30 ug/ml), serum dilution (1:40) and anti-human conjugate working dilution (1:1500), were determined by a check board titration method. The UMELISA CMV was compared with the latex agglutination test for antibodies to CMV (Dupont de Nemours) and with an indiret immunofluorescent method. The results have showed the high coincidence, sensitivity and especificity of the proposed assay regarding the two methods compared with, and supporting its use either for a blood donors screening or in the serological diagnosis of this infection by paired serum samples.
publishDate 1992
dc.date.none.fl_str_mv 1992-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28890
url https://www.revistas.usp.br/rimtsp/article/view/28890
dc.language.iso.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28890/30744
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 1 (1992); 43-47
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 1 (1992); 43-47
Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 1 (1992); 43-47
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
_version_ 1798951639382491136

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