Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity

Detalhes bibliográficos
Autor(a) principal: Costa, Carolina Rodrigues
Data de Publicação: 2010
Outros Autores: Passos, Xisto Sena, Souza, Lúcia Kioko Hasimoto e, Lucena, Percilia de Andrade, Fernandes, Orionalda de Fátima Lisboa, Silva, Maria do Rosário Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31321
Resumo: Phospholipase and proteinase production and the ability of adhesion to buccal epithelial cells (BEC) of 112 Candida isolates originated from oral cavity of HIV infected patients and from blood and catheter of intensive care unit patients were investigated. The proteinase production was detected by inoculation into bovine serum albumin (BSA) agar and the phospholipase activity was performed using egg yolk emulsion. A yeast suspension of each test strain was incubated with buccal epithelial cells and the number of adherence yeast to epithelial cells was counted. A percentage of 88.1% and 55.9% of Candida albicans and 69.8% and 37.7% of non-albicans Candida isolates produced proteinase and phospholipase, respectively. Non-albicans Candida isolated from catheter were more proteolytic than C. albicans isolates. Blood isolates were more proteolytic than catheter and oral cavity isolates while oral cavity isolates produced more phospholipase than those from blood and catheter. C. albicans isolates from oral cavity and from catheter were more adherent to BEC than non-albicans Candida isolates, but the adhesion was not different among the three sources analyzed. The results indicated differences in the production of phospholipase and proteinase and in the ability of adhesion to BEC among Candida spp. isolates from different sources. This study suggests that the pathogenicity of Candida can be correlated with the infected site.
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spelling Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity Diferenças na produção de exoenzimas e habilidade de aderência entre isolados de espécies de Candida provenientes do cateter, sangue e cavidade bucal Adherence abilityCandidaExoenzyme activity Phospholipase and proteinase production and the ability of adhesion to buccal epithelial cells (BEC) of 112 Candida isolates originated from oral cavity of HIV infected patients and from blood and catheter of intensive care unit patients were investigated. The proteinase production was detected by inoculation into bovine serum albumin (BSA) agar and the phospholipase activity was performed using egg yolk emulsion. A yeast suspension of each test strain was incubated with buccal epithelial cells and the number of adherence yeast to epithelial cells was counted. A percentage of 88.1% and 55.9% of Candida albicans and 69.8% and 37.7% of non-albicans Candida isolates produced proteinase and phospholipase, respectively. Non-albicans Candida isolated from catheter were more proteolytic than C. albicans isolates. Blood isolates were more proteolytic than catheter and oral cavity isolates while oral cavity isolates produced more phospholipase than those from blood and catheter. C. albicans isolates from oral cavity and from catheter were more adherent to BEC than non-albicans Candida isolates, but the adhesion was not different among the three sources analyzed. The results indicated differences in the production of phospholipase and proteinase and in the ability of adhesion to BEC among Candida spp. isolates from different sources. This study suggests that the pathogenicity of Candida can be correlated with the infected site. A produção de proteinase e fosfolipase e habilidade de adesão à célula epitelial bucal de 112 isolados de Candida originadas da cavidade bucal de pacientes infectados pelo HIV e de sangue e cateter de pacientes hospitalizados foram investigados. A produção de proteinase foi detectada por inoculação em ágar soro albumina bovina e a atividade de fosfolipase foi realizada usando emulsão de gema de ovo. A suspensão de levedura de cada isolado foi incubada com célula epitelial e o número de leveduras aderidas a célula epitelial foi contada. Uma porcentagem de 88,1 e 55,9% de C. albicans e 69,8 e 37,7% de isolados de Candida não albicans produziram proteinase e fosfolipase, respectivamente. Candida não albicans obtidas do cateter foram mais proteolíticos que isolados de Candida albicans (p < 0,001). Isolados do sangue foram mais proteolíticos do que isolados do cateter e cavidade bucal, enquanto isolados da cavidade bucal produziram mais fosfolipase do que aqueles isolados do sangue e cateter. C. albicans isoladas da cavidade bucal e do cateter foram mais aderentes à célula epitelial bucal do que isolados de Candida não albicans, mas não houve diferença na adesão entre os três locais analisados. Os resultados indicaram diferenças na produção de fosfolipase e proteinase e na habilidade de adesão à célula epitelial bucal entre os isolados de Candida das diferentes fontes. Este estudo sugere que a patogenicidade de Candida spp pode estar correlacionada ao local infectado. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31321Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 No. 3 (2010); 139-143 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 Núm. 3 (2010); 139-143 Revista do Instituto de Medicina Tropical de São Paulo; v. 52 n. 3 (2010); 139-143 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31321/33206Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCosta, Carolina RodriguesPassos, Xisto SenaSouza, Lúcia Kioko Hasimoto eLucena, Percilia de AndradeFernandes, Orionalda de Fátima LisboaSilva, Maria do Rosário Rodrigues2012-07-07T19:31:14Zoai:revistas.usp.br:article/31321Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2012-07-07T19:31:14Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)false
dc.title.none.fl_str_mv Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity
Diferenças na produção de exoenzimas e habilidade de aderência entre isolados de espécies de Candida provenientes do cateter, sangue e cavidade bucal
title Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity
spellingShingle Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity
Costa, Carolina Rodrigues
Adherence ability
Candida
Exoenzyme activity
title_short Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity
title_full Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity
title_fullStr Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity
title_full_unstemmed Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity
title_sort Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity
author Costa, Carolina Rodrigues
author_facet Costa, Carolina Rodrigues
Passos, Xisto Sena
Souza, Lúcia Kioko Hasimoto e
Lucena, Percilia de Andrade
Fernandes, Orionalda de Fátima Lisboa
Silva, Maria do Rosário Rodrigues
author_role author
author2 Passos, Xisto Sena
Souza, Lúcia Kioko Hasimoto e
Lucena, Percilia de Andrade
Fernandes, Orionalda de Fátima Lisboa
Silva, Maria do Rosário Rodrigues
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Carolina Rodrigues
Passos, Xisto Sena
Souza, Lúcia Kioko Hasimoto e
Lucena, Percilia de Andrade
Fernandes, Orionalda de Fátima Lisboa
Silva, Maria do Rosário Rodrigues
dc.subject.por.fl_str_mv Adherence ability
Candida
Exoenzyme activity
topic Adherence ability
Candida
Exoenzyme activity
description Phospholipase and proteinase production and the ability of adhesion to buccal epithelial cells (BEC) of 112 Candida isolates originated from oral cavity of HIV infected patients and from blood and catheter of intensive care unit patients were investigated. The proteinase production was detected by inoculation into bovine serum albumin (BSA) agar and the phospholipase activity was performed using egg yolk emulsion. A yeast suspension of each test strain was incubated with buccal epithelial cells and the number of adherence yeast to epithelial cells was counted. A percentage of 88.1% and 55.9% of Candida albicans and 69.8% and 37.7% of non-albicans Candida isolates produced proteinase and phospholipase, respectively. Non-albicans Candida isolated from catheter were more proteolytic than C. albicans isolates. Blood isolates were more proteolytic than catheter and oral cavity isolates while oral cavity isolates produced more phospholipase than those from blood and catheter. C. albicans isolates from oral cavity and from catheter were more adherent to BEC than non-albicans Candida isolates, but the adhesion was not different among the three sources analyzed. The results indicated differences in the production of phospholipase and proteinase and in the ability of adhesion to BEC among Candida spp. isolates from different sources. This study suggests that the pathogenicity of Candida can be correlated with the infected site.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31321
url https://www.revistas.usp.br/rimtsp/article/view/31321
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/31321/33206
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 No. 3 (2010); 139-143
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 52 Núm. 3 (2010); 139-143
Revista do Instituto de Medicina Tropical de São Paulo; v. 52 n. 3 (2010); 139-143
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
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