Evaluation of a technique of fecal sedimentation in test tubes
Autor(a) principal: | |
---|---|
Data de Publicação: | 1994 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/29139 |
Resumo: | The efficiency of sedimentation in test tubes for the detection of eggs of Ancylostomidae, Trichuris trichiura and Ascaris lumbricoides was tested in 80 mm-high columns of fecal suspensions. Dilutions of 2.0%, 1.5% and 1.0% were tested by using samples containing, respectively, 12.5,25.0, 50.0,100.0,150.0 and 200.0 eggs per gram (epg) of each helminth under study. Efficiency was evaluated in terms of frequency of positive results and eggs per slide of the sediment (0.078 ml). Both the frequencies of positive results and egg counts per slide increased with epg values, but were not apparently dependent from suspension dilutions within the range assessed. Sediments of 1.0% fecal suspensions were positive in 80.0% or more of the cases for epg values of 150.0. It is inferred that dilution values are not critical. Thus, it is not necessary to determinem fecal weights or volumes instrumentally. Considering overall results, it may be concluded that 1.0% fecal dilutions should be recommended for best results both in terms transparency of the preparations and chances of recovering eggs, particularly when egg counts are low. |
id |
IMT-1_64cda8caaba669322861406dc2ae4abb |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/29139 |
network_acronym_str |
IMT-1 |
network_name_str |
Revista do Instituto de Medicina Tropical de São Paulo |
repository_id_str |
|
spelling |
Evaluation of a technique of fecal sedimentation in test tubes Avaliação de técnica de sedimentação em tubos Sedimentação em tubosTécnicas de sedimentaçãoCoroscopia parasitológicaOvos de helmintos The efficiency of sedimentation in test tubes for the detection of eggs of Ancylostomidae, Trichuris trichiura and Ascaris lumbricoides was tested in 80 mm-high columns of fecal suspensions. Dilutions of 2.0%, 1.5% and 1.0% were tested by using samples containing, respectively, 12.5,25.0, 50.0,100.0,150.0 and 200.0 eggs per gram (epg) of each helminth under study. Efficiency was evaluated in terms of frequency of positive results and eggs per slide of the sediment (0.078 ml). Both the frequencies of positive results and egg counts per slide increased with epg values, but were not apparently dependent from suspension dilutions within the range assessed. Sediments of 1.0% fecal suspensions were positive in 80.0% or more of the cases for epg values of 150.0. It is inferred that dilution values are not critical. Thus, it is not necessary to determinem fecal weights or volumes instrumentally. Considering overall results, it may be concluded that 1.0% fecal dilutions should be recommended for best results both in terms transparency of the preparations and chances of recovering eggs, particularly when egg counts are low. O rendimento de sedimentação em tubos, aplicada à pesquisa de ovos de Ancylostomidae, Trichuris trichura e Ascaris lumbricoides foi estudado em suspensões fecais diluídas a 2,0%; 1,5% e 1,0% em colunas de 80 mm de altura. De cada um dos helmintos citados, as amostras continham quantidades correspondentes a 12,5; 25,0; 50,0; 100,0; 150,0 e 200,0 ovos por grama de fezes (opg). Avaliou-se o rendimento do processo em termos de freqüências de resultados positivos e de contagens de ovos por lâmina de sedimento (0,078 ml). Ambos aumentaram com os valores de opg. As várias diluições não influíram nos graus de positividade ou nas contagens de ovos por lâmina de sedimento. Os sedimentos de suspensões fecais diluídas a 1,0% (quando o valor de opg era de 150,0). Em trabalho rotineiro é desnecessária a determinação instrumental das quantidades de fezes usadas nas suspensões. Diluições em torno de 1,0% representam um compromisso entre bom rendimento e limpidez dos esfregaços, especialmente quando os valores de opg forem baixos. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1994-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29139Revista do Instituto de Medicina Tropical de São Paulo; Vol. 36 No. 2 (1994); 153-156 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 36 Núm. 2 (1994); 153-156 Revista do Instituto de Medicina Tropical de São Paulo; v. 36 n. 2 (1994); 153-156 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/29139/30996Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessMoitinho, Maria da Luz RibeiroFerreira, Cláudio Santos2012-07-02T01:37:26Zoai:revistas.usp.br:article/29139Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:53.588554Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Evaluation of a technique of fecal sedimentation in test tubes Avaliação de técnica de sedimentação em tubos |
title |
Evaluation of a technique of fecal sedimentation in test tubes |
spellingShingle |
Evaluation of a technique of fecal sedimentation in test tubes Moitinho, Maria da Luz Ribeiro Sedimentação em tubos Técnicas de sedimentação Coroscopia parasitológica Ovos de helmintos |
title_short |
Evaluation of a technique of fecal sedimentation in test tubes |
title_full |
Evaluation of a technique of fecal sedimentation in test tubes |
title_fullStr |
Evaluation of a technique of fecal sedimentation in test tubes |
title_full_unstemmed |
Evaluation of a technique of fecal sedimentation in test tubes |
title_sort |
Evaluation of a technique of fecal sedimentation in test tubes |
author |
Moitinho, Maria da Luz Ribeiro |
author_facet |
Moitinho, Maria da Luz Ribeiro Ferreira, Cláudio Santos |
author_role |
author |
author2 |
Ferreira, Cláudio Santos |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Moitinho, Maria da Luz Ribeiro Ferreira, Cláudio Santos |
dc.subject.por.fl_str_mv |
Sedimentação em tubos Técnicas de sedimentação Coroscopia parasitológica Ovos de helmintos |
topic |
Sedimentação em tubos Técnicas de sedimentação Coroscopia parasitológica Ovos de helmintos |
description |
The efficiency of sedimentation in test tubes for the detection of eggs of Ancylostomidae, Trichuris trichiura and Ascaris lumbricoides was tested in 80 mm-high columns of fecal suspensions. Dilutions of 2.0%, 1.5% and 1.0% were tested by using samples containing, respectively, 12.5,25.0, 50.0,100.0,150.0 and 200.0 eggs per gram (epg) of each helminth under study. Efficiency was evaluated in terms of frequency of positive results and eggs per slide of the sediment (0.078 ml). Both the frequencies of positive results and egg counts per slide increased with epg values, but were not apparently dependent from suspension dilutions within the range assessed. Sediments of 1.0% fecal suspensions were positive in 80.0% or more of the cases for epg values of 150.0. It is inferred that dilution values are not critical. Thus, it is not necessary to determinem fecal weights or volumes instrumentally. Considering overall results, it may be concluded that 1.0% fecal dilutions should be recommended for best results both in terms transparency of the preparations and chances of recovering eggs, particularly when egg counts are low. |
publishDate |
1994 |
dc.date.none.fl_str_mv |
1994-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29139 |
url |
https://www.revistas.usp.br/rimtsp/article/view/29139 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29139/30996 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 36 No. 2 (1994); 153-156 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 36 Núm. 2 (1994); 153-156 Revista do Instituto de Medicina Tropical de São Paulo; v. 36 n. 2 (1994); 153-156 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
_version_ |
1798951640616665088 |