Prevention of electrocardiographic and histopathologic alterations in the murine model of Chagas' disease by preinoculation of an attenuated Trypanosoma cruzi strain
Autor(a) principal: | |
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Data de Publicação: | 1989 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28671 |
Resumo: | The effects of infection with Trypanosoma cruzi on the electrocardiographic tracings of mice were studied in 4.groups of animals: (1) normal; (2) infected with a pathogenic T. cruzi strain (TS COB); (3) immunized with 3 intraperitoneal inocula of 10(6) attenuated T. cruzi epimastigotes (TCC) and (4) immunized-infected, which sequentially received the treatments of groups 3 and 2. Infection and protection were confirmed by xenodiagnosis and histopathology. Isolated alterations such as extrasystolia, 1st degree atrioventricular block, arrhythmia and ST elevation were observed in normal as well as infected mice. However, tracings taken repeatedly on each mouse over a 293 day period revealed a set of alterations which were more frequently seen in infected (14/22) than in normal (4/27) animals (p = 0.00048). These alterations consisted of supraventricular tachycardia, sinus bradycardia and persisting, first degree AV blocks, often associated to pacemaker changes. Inoculation of attenuated T. cruzi (group 3) did not increase these alterations (2/27 mice) but significantly prevented their development after challenge with the pathogenic strain (1/19 versus 14/22 mice, p = 0.000095). Thus, preimmunization reduced not only parasitemia but also a pathogenic consequence of T. cruzi infection. This evidence is relevant for immunoprevention studies against Chagas' disease. |
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Prevention of electrocardiographic and histopathologic alterations in the murine model of Chagas' disease by preinoculation of an attenuated Trypanosoma cruzi strain Prevenção das alterações eletrocardiográficas e histopatológicas em modelo murino de doença de Chagas através da pré-inoculação de cepa atenuada de Trypanosoma cruzi Chagas' diseaseElectrocardiogramHistopathologyHeartImmunization The effects of infection with Trypanosoma cruzi on the electrocardiographic tracings of mice were studied in 4.groups of animals: (1) normal; (2) infected with a pathogenic T. cruzi strain (TS COB); (3) immunized with 3 intraperitoneal inocula of 10(6) attenuated T. cruzi epimastigotes (TCC) and (4) immunized-infected, which sequentially received the treatments of groups 3 and 2. Infection and protection were confirmed by xenodiagnosis and histopathology. Isolated alterations such as extrasystolia, 1st degree atrioventricular block, arrhythmia and ST elevation were observed in normal as well as infected mice. However, tracings taken repeatedly on each mouse over a 293 day period revealed a set of alterations which were more frequently seen in infected (14/22) than in normal (4/27) animals (p = 0.00048). These alterations consisted of supraventricular tachycardia, sinus bradycardia and persisting, first degree AV blocks, often associated to pacemaker changes. Inoculation of attenuated T. cruzi (group 3) did not increase these alterations (2/27 mice) but significantly prevented their development after challenge with the pathogenic strain (1/19 versus 14/22 mice, p = 0.000095). Thus, preimmunization reduced not only parasitemia but also a pathogenic consequence of T. cruzi infection. This evidence is relevant for immunoprevention studies against Chagas' disease. Os efeitos da infecção do Trypanosoma cruzi nos traçados eletrocardiográficos de camundongos foram estudados em quatro grupos de animais: 1. normal; 2. infectado com cepa patogênica de T. cruzi (TS.COB); 3. imunizados com três inoculações intra-peritoneais de 10(6) epimastigotas de T. cruzi atenuadas (TCC) e 4. imunizados e infectados, que receberam seqüencialmente os tratamentos dos grupos 3 e 2. A infecção e proteção foram confirmadas por xenodiagnóstico e histopatologia. Alterações isoladas tais como extrassistolia, bloqueio de primeiro grau átrio-ventricular, arritmia e elevação do ST. foram observados tanto em camundongos normais como infectados. Entretanto, os traçados tomados de maneira repetida em cada camundongo durante um período de 293 dias revelaram conjunto de alterações que foram vistas mais freqüentemente em animais infectados (14/22) que nos normais (4/27) (p = 0.00048). Estas alterações consistiram de taquicardia supra-ventricular, bradicardia sinusal e bloqueios de primeiro grau AV, persistentes, freqüentemente associadas a alterações do "pacemaker". A inoculação da cepa atenuada de T. cruzi(grupo 3) não aumentou estas alterações (2/27) mas preveniu de maneira significante o seu desenvolvimento após o desafio com a cepa patogênica (1/19 versus 14/22 camundon-gos, p = 0.000095). Portanto, a pré-imuniza-çâo reduziu não somente a parasitemia mas a conseqüência patogênica da infecção pelo T. cruzi. Esta evidência é relevante nos estudos de imunoprevenção em doença de Chagas. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1989-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28671Revista do Instituto de Medicina Tropical de São Paulo; Vol. 31 No. 4 (1989); 248-255 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 31 Núm. 4 (1989); 248-255 Revista do Instituto de Medicina Tropical de São Paulo; v. 31 n. 4 (1989); 248-255 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28671/30524Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCuneo, Carlos AlbertoMolina de Raspi, EmmaBasombrio, Miguel Angel2012-07-02T01:10:24Zoai:revistas.usp.br:article/28671Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:25.105768Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Prevention of electrocardiographic and histopathologic alterations in the murine model of Chagas' disease by preinoculation of an attenuated Trypanosoma cruzi strain Prevenção das alterações eletrocardiográficas e histopatológicas em modelo murino de doença de Chagas através da pré-inoculação de cepa atenuada de Trypanosoma cruzi |
title |
Prevention of electrocardiographic and histopathologic alterations in the murine model of Chagas' disease by preinoculation of an attenuated Trypanosoma cruzi strain |
spellingShingle |
Prevention of electrocardiographic and histopathologic alterations in the murine model of Chagas' disease by preinoculation of an attenuated Trypanosoma cruzi strain Cuneo, Carlos Alberto Chagas' disease Electrocardiogram Histopathology Heart Immunization |
title_short |
Prevention of electrocardiographic and histopathologic alterations in the murine model of Chagas' disease by preinoculation of an attenuated Trypanosoma cruzi strain |
title_full |
Prevention of electrocardiographic and histopathologic alterations in the murine model of Chagas' disease by preinoculation of an attenuated Trypanosoma cruzi strain |
title_fullStr |
Prevention of electrocardiographic and histopathologic alterations in the murine model of Chagas' disease by preinoculation of an attenuated Trypanosoma cruzi strain |
title_full_unstemmed |
Prevention of electrocardiographic and histopathologic alterations in the murine model of Chagas' disease by preinoculation of an attenuated Trypanosoma cruzi strain |
title_sort |
Prevention of electrocardiographic and histopathologic alterations in the murine model of Chagas' disease by preinoculation of an attenuated Trypanosoma cruzi strain |
author |
Cuneo, Carlos Alberto |
author_facet |
Cuneo, Carlos Alberto Molina de Raspi, Emma Basombrio, Miguel Angel |
author_role |
author |
author2 |
Molina de Raspi, Emma Basombrio, Miguel Angel |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Cuneo, Carlos Alberto Molina de Raspi, Emma Basombrio, Miguel Angel |
dc.subject.por.fl_str_mv |
Chagas' disease Electrocardiogram Histopathology Heart Immunization |
topic |
Chagas' disease Electrocardiogram Histopathology Heart Immunization |
description |
The effects of infection with Trypanosoma cruzi on the electrocardiographic tracings of mice were studied in 4.groups of animals: (1) normal; (2) infected with a pathogenic T. cruzi strain (TS COB); (3) immunized with 3 intraperitoneal inocula of 10(6) attenuated T. cruzi epimastigotes (TCC) and (4) immunized-infected, which sequentially received the treatments of groups 3 and 2. Infection and protection were confirmed by xenodiagnosis and histopathology. Isolated alterations such as extrasystolia, 1st degree atrioventricular block, arrhythmia and ST elevation were observed in normal as well as infected mice. However, tracings taken repeatedly on each mouse over a 293 day period revealed a set of alterations which were more frequently seen in infected (14/22) than in normal (4/27) animals (p = 0.00048). These alterations consisted of supraventricular tachycardia, sinus bradycardia and persisting, first degree AV blocks, often associated to pacemaker changes. Inoculation of attenuated T. cruzi (group 3) did not increase these alterations (2/27 mice) but significantly prevented their development after challenge with the pathogenic strain (1/19 versus 14/22 mice, p = 0.000095). Thus, preimmunization reduced not only parasitemia but also a pathogenic consequence of T. cruzi infection. This evidence is relevant for immunoprevention studies against Chagas' disease. |
publishDate |
1989 |
dc.date.none.fl_str_mv |
1989-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28671 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28671 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28671/30524 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 31 No. 4 (1989); 248-255 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 31 Núm. 4 (1989); 248-255 Revista do Instituto de Medicina Tropical de São Paulo; v. 31 n. 4 (1989); 248-255 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951638208086016 |