Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/204777 |
Resumo: | Syphilis is caused by the bacterium Treponema pallidum. The diagnosis is based on clinical data and serological analysis; however, the sensitivity and specificity of such tests may vary depending on the type of test and stage of the infection. In order to overcome this premise, this study utilized the polymerase chain reaction (PCR) for the detection of T. pallidum DNA in whole blood samples of patients with syphilis. The blood samples from patients with or without symptoms of syphilis, but with positive results in enzyme-linked immunosorbent assay (ELISA), were included in this study. A venereal disease research laboratory (VDRL) test was performed for all collected sera samples. For PCR, the T. pallidum DNA was extracted from the collected blood samples and a specific primer set was designed to amplify 131 nucleotides of polA (Tp0105). The specificity of the primers was evaluated with the DNA of 17 different pathogens. From a total of 314 blood samples reactive in ELISA, 58.2% (183/314) of the samples were reactive in the VDRL test. In the PCR, 54% (168/314) of the ELISA-reactive samples were positive. In both tests (VDRL and PCR) 104 samples were positive. Of 104 positive samples for both tests, 71 were at the latent stage. Based on these results, it can be concluded that PCR with the designed set of primers can be utilized as a diagnostic method for T. pallidum detection in blood samples of patients with syphilis, especially those with latent infection. In addition, it can be utilized as a supplement for serological methods to improve the diagnosis of syphilis. |
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Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reactionSyphilispolA geneTreponema pallidum DNAWhole bloodSyphilis is caused by the bacterium Treponema pallidum. The diagnosis is based on clinical data and serological analysis; however, the sensitivity and specificity of such tests may vary depending on the type of test and stage of the infection. In order to overcome this premise, this study utilized the polymerase chain reaction (PCR) for the detection of T. pallidum DNA in whole blood samples of patients with syphilis. The blood samples from patients with or without symptoms of syphilis, but with positive results in enzyme-linked immunosorbent assay (ELISA), were included in this study. A venereal disease research laboratory (VDRL) test was performed for all collected sera samples. For PCR, the T. pallidum DNA was extracted from the collected blood samples and a specific primer set was designed to amplify 131 nucleotides of polA (Tp0105). The specificity of the primers was evaluated with the DNA of 17 different pathogens. From a total of 314 blood samples reactive in ELISA, 58.2% (183/314) of the samples were reactive in the VDRL test. In the PCR, 54% (168/314) of the ELISA-reactive samples were positive. In both tests (VDRL and PCR) 104 samples were positive. Of 104 positive samples for both tests, 71 were at the latent stage. Based on these results, it can be concluded that PCR with the designed set of primers can be utilized as a diagnostic method for T. pallidum detection in blood samples of patients with syphilis, especially those with latent infection. In addition, it can be utilized as a supplement for serological methods to improve the diagnosis of syphilis.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2022-11-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/20477710.1590/S1678-9946202264075Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e75Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e75Revista do Instituto de Medicina Tropical de São Paulo; v. 64 (2022); e751678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/204777/188405Copyright (c) 2022 Júlio Henrique Ferreira de Sá Queiroz, Maisa Estopa Correa, Tiago da Silva Ferreira, Michele Ferreira Marques, Marcelo dos Santos Barbosa, Silvana Beutinger Marchioro, Simone Simionattohttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessQueiroz, Júlio Henrique Ferreira de Sá Correa, Maisa Estopa Ferreira, Tiago da Silva Marques, Michele Ferreira Barbosa, Marcelo dos Santos Marchioro, Silvana Beutinger Simionatto, Simone 2022-11-22T17:28:36Zoai:revistas.usp.br:article/204777Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:54:11.301884Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction |
title |
Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction |
spellingShingle |
Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction Queiroz, Júlio Henrique Ferreira de Sá Syphilis polA gene Treponema pallidum DNA Whole blood |
title_short |
Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction |
title_full |
Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction |
title_fullStr |
Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction |
title_full_unstemmed |
Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction |
title_sort |
Detection of Treponema pallidum in whole blood samples of patients with syphilis by the polymerase chain reaction |
author |
Queiroz, Júlio Henrique Ferreira de Sá |
author_facet |
Queiroz, Júlio Henrique Ferreira de Sá Correa, Maisa Estopa Ferreira, Tiago da Silva Marques, Michele Ferreira Barbosa, Marcelo dos Santos Marchioro, Silvana Beutinger Simionatto, Simone |
author_role |
author |
author2 |
Correa, Maisa Estopa Ferreira, Tiago da Silva Marques, Michele Ferreira Barbosa, Marcelo dos Santos Marchioro, Silvana Beutinger Simionatto, Simone |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Queiroz, Júlio Henrique Ferreira de Sá Correa, Maisa Estopa Ferreira, Tiago da Silva Marques, Michele Ferreira Barbosa, Marcelo dos Santos Marchioro, Silvana Beutinger Simionatto, Simone |
dc.subject.por.fl_str_mv |
Syphilis polA gene Treponema pallidum DNA Whole blood |
topic |
Syphilis polA gene Treponema pallidum DNA Whole blood |
description |
Syphilis is caused by the bacterium Treponema pallidum. The diagnosis is based on clinical data and serological analysis; however, the sensitivity and specificity of such tests may vary depending on the type of test and stage of the infection. In order to overcome this premise, this study utilized the polymerase chain reaction (PCR) for the detection of T. pallidum DNA in whole blood samples of patients with syphilis. The blood samples from patients with or without symptoms of syphilis, but with positive results in enzyme-linked immunosorbent assay (ELISA), were included in this study. A venereal disease research laboratory (VDRL) test was performed for all collected sera samples. For PCR, the T. pallidum DNA was extracted from the collected blood samples and a specific primer set was designed to amplify 131 nucleotides of polA (Tp0105). The specificity of the primers was evaluated with the DNA of 17 different pathogens. From a total of 314 blood samples reactive in ELISA, 58.2% (183/314) of the samples were reactive in the VDRL test. In the PCR, 54% (168/314) of the ELISA-reactive samples were positive. In both tests (VDRL and PCR) 104 samples were positive. Of 104 positive samples for both tests, 71 were at the latent stage. Based on these results, it can be concluded that PCR with the designed set of primers can be utilized as a diagnostic method for T. pallidum detection in blood samples of patients with syphilis, especially those with latent infection. In addition, it can be utilized as a supplement for serological methods to improve the diagnosis of syphilis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/204777 10.1590/S1678-9946202264075 |
url |
https://www.revistas.usp.br/rimtsp/article/view/204777 |
identifier_str_mv |
10.1590/S1678-9946202264075 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/204777/188405 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e75 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e75 Revista do Instituto de Medicina Tropical de São Paulo; v. 64 (2022); e75 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951659421827072 |