Detection of hepatitis A antibodies by ELISA using saliva as clinical samples
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30439 |
Resumo: | The possibility of detecting acute infection and immunity using body fluids that are easier to collect than blood, mainly in children, would facilitate the investigation and follow-up of outbreaks of hepatitis A (HAV). Our study was carried out to evaluate the detection of anti-HAV IgM, IgA and total antibodies in saliva using serum samples as reference. Forty three paired serum and saliva samples were analyzed. From this total, 24 samples were obtained from children and 1 from one adult during the course of acute hepatitis A; an additional 18 samples were obtained from health professionals from Adolfo Lutz Institute. The sensitivity to detect anti-HAV IgM was 100% (95%CI: 79.1 to 100.0%), employing saliva as clinical samples. In detecting anti-HAV IgA, the sensitivity was 80.8% (95%CI: 60.0 to 92.7%) and for the total antibodies was 82.1% (95%CI: 62.4 to 93.2%). The specificity was 100% for each. The rate of agreement was high comparing the results of serum and saliva samples for detecting HAV antibodies. We conclude that saliva is an acceptable alternative specimen for diagnosing acute hepatitis A infection, and for screening individuals to receive hepatitis A vaccine or immunoglobulin. |
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Revista do Instituto de Medicina Tropical de São Paulo |
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Detection of hepatitis A antibodies by ELISA using saliva as clinical samples Detecção de anticorpos anti-VHA em amostras de saliva utilizando teste imuno-enzimático HepatitiSalAntibodVacc The possibility of detecting acute infection and immunity using body fluids that are easier to collect than blood, mainly in children, would facilitate the investigation and follow-up of outbreaks of hepatitis A (HAV). Our study was carried out to evaluate the detection of anti-HAV IgM, IgA and total antibodies in saliva using serum samples as reference. Forty three paired serum and saliva samples were analyzed. From this total, 24 samples were obtained from children and 1 from one adult during the course of acute hepatitis A; an additional 18 samples were obtained from health professionals from Adolfo Lutz Institute. The sensitivity to detect anti-HAV IgM was 100% (95%CI: 79.1 to 100.0%), employing saliva as clinical samples. In detecting anti-HAV IgA, the sensitivity was 80.8% (95%CI: 60.0 to 92.7%) and for the total antibodies was 82.1% (95%CI: 62.4 to 93.2%). The specificity was 100% for each. The rate of agreement was high comparing the results of serum and saliva samples for detecting HAV antibodies. We conclude that saliva is an acceptable alternative specimen for diagnosing acute hepatitis A infection, and for screening individuals to receive hepatitis A vaccine or immunoglobulin. A possibilidade de identificar infecções presentes ou passadas utilizando fluidos corpóreos que seriam mais facilmente coletados do que o sangue, principalmente em crianças, facilitaria grandemente a investigação e o acompanhamento de surtos de hepatite A, que ocorrem com muita freqüência em nosso meio. Nosso estudo foi desenvolvido com a finalidade de avaliar a detecção dos anticorpos anti-VHA, da classe IgA, IgM, e anticorpos totais em amostras de saliva, usando amostras de soro como padrão. Foram estudadas 43 amostras pareadas de saliva e de soro, colhidas de 24 crianças e de um adulto durante um surto de hepatite A, e de 18 funcionários do Instituto Adolfo Lutz. Empregando saliva como amostra clínica, a sensibilidade para a detecção de anti-VHA IgM foi de 100,0%, de anti-VHA IgA foi de 80,8% e de anti-VHA total foi de 82,1%. Não houve nenhum resultado falso-positivo, sendo a especificidade de 100%. A concordância foi alta entre os resultados das amostras de saliva e soro na detecção dos anticorpos, indicando que amostras de saliva podem ser utilizadas no diagnóstico de infecção aguda pelo VHA e na seleção de indivíduos para vacinação contra o VHA, para conter surtos. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2000-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30439Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 No. 4 (2000); 197-200 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 Núm. 4 (2000); 197-200 Revista do Instituto de Medicina Tropical de São Paulo; v. 42 n. 4 (2000); 197-200 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30439/32323Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessOBA, Isabel TakanoSPINA, Angela Maria MirandaSARACENI, Cláudia PataraLEMOS, Marcílio FigueiredoSENHORAS, Rita de Cássia Ferreira AndradeMOREIRA, Regina CéliaGRANATO, Celso Francisco Hernandes2012-07-07T09:35:24Zoai:revistas.usp.br:article/30439Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:12.693394Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples Detecção de anticorpos anti-VHA em amostras de saliva utilizando teste imuno-enzimático |
title |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples |
spellingShingle |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples OBA, Isabel Takano Hepatiti Sal Antibod Vacc |
title_short |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples |
title_full |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples |
title_fullStr |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples |
title_full_unstemmed |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples |
title_sort |
Detection of hepatitis A antibodies by ELISA using saliva as clinical samples |
author |
OBA, Isabel Takano |
author_facet |
OBA, Isabel Takano SPINA, Angela Maria Miranda SARACENI, Cláudia Patara LEMOS, Marcílio Figueiredo SENHORAS, Rita de Cássia Ferreira Andrade MOREIRA, Regina Célia GRANATO, Celso Francisco Hernandes |
author_role |
author |
author2 |
SPINA, Angela Maria Miranda SARACENI, Cláudia Patara LEMOS, Marcílio Figueiredo SENHORAS, Rita de Cássia Ferreira Andrade MOREIRA, Regina Célia GRANATO, Celso Francisco Hernandes |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
OBA, Isabel Takano SPINA, Angela Maria Miranda SARACENI, Cláudia Patara LEMOS, Marcílio Figueiredo SENHORAS, Rita de Cássia Ferreira Andrade MOREIRA, Regina Célia GRANATO, Celso Francisco Hernandes |
dc.subject.por.fl_str_mv |
Hepatiti Sal Antibod Vacc |
topic |
Hepatiti Sal Antibod Vacc |
description |
The possibility of detecting acute infection and immunity using body fluids that are easier to collect than blood, mainly in children, would facilitate the investigation and follow-up of outbreaks of hepatitis A (HAV). Our study was carried out to evaluate the detection of anti-HAV IgM, IgA and total antibodies in saliva using serum samples as reference. Forty three paired serum and saliva samples were analyzed. From this total, 24 samples were obtained from children and 1 from one adult during the course of acute hepatitis A; an additional 18 samples were obtained from health professionals from Adolfo Lutz Institute. The sensitivity to detect anti-HAV IgM was 100% (95%CI: 79.1 to 100.0%), employing saliva as clinical samples. In detecting anti-HAV IgA, the sensitivity was 80.8% (95%CI: 60.0 to 92.7%) and for the total antibodies was 82.1% (95%CI: 62.4 to 93.2%). The specificity was 100% for each. The rate of agreement was high comparing the results of serum and saliva samples for detecting HAV antibodies. We conclude that saliva is an acceptable alternative specimen for diagnosing acute hepatitis A infection, and for screening individuals to receive hepatitis A vaccine or immunoglobulin. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30439 |
url |
https://www.revistas.usp.br/rimtsp/article/view/30439 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30439/32323 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 No. 4 (2000); 197-200 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 Núm. 4 (2000); 197-200 Revista do Instituto de Medicina Tropical de São Paulo; v. 42 n. 4 (2000); 197-200 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951642795606016 |