Detection of hepatitis A antibodies by ELISA using saliva as clinical samples

Detalhes bibliográficos
Autor(a) principal: OBA, Isabel Takano
Data de Publicação: 2000
Outros Autores: SPINA, Angela Maria Miranda, SARACENI, Cláudia Patara, LEMOS, Marcílio Figueiredo, SENHORAS, Rita de Cássia Ferreira Andrade, MOREIRA, Regina Célia, GRANATO, Celso Francisco Hernandes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30439
Resumo: The possibility of detecting acute infection and immunity using body fluids that are easier to collect than blood, mainly in children, would facilitate the investigation and follow-up of outbreaks of hepatitis A (HAV). Our study was carried out to evaluate the detection of anti-HAV IgM, IgA and total antibodies in saliva using serum samples as reference. Forty three paired serum and saliva samples were analyzed. From this total, 24 samples were obtained from children and 1 from one adult during the course of acute hepatitis A; an additional 18 samples were obtained from health professionals from Adolfo Lutz Institute. The sensitivity to detect anti-HAV IgM was 100% (95%CI: 79.1 to 100.0%), employing saliva as clinical samples. In detecting anti-HAV IgA, the sensitivity was 80.8% (95%CI: 60.0 to 92.7%) and for the total antibodies was 82.1% (95%CI: 62.4 to 93.2%). The specificity was 100% for each. The rate of agreement was high comparing the results of serum and saliva samples for detecting HAV antibodies. We conclude that saliva is an acceptable alternative specimen for diagnosing acute hepatitis A infection, and for screening individuals to receive hepatitis A vaccine or immunoglobulin.
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spelling Detection of hepatitis A antibodies by ELISA using saliva as clinical samples Detecção de anticorpos anti-VHA em amostras de saliva utilizando teste imuno-enzimático HepatitiSalAntibodVacc The possibility of detecting acute infection and immunity using body fluids that are easier to collect than blood, mainly in children, would facilitate the investigation and follow-up of outbreaks of hepatitis A (HAV). Our study was carried out to evaluate the detection of anti-HAV IgM, IgA and total antibodies in saliva using serum samples as reference. Forty three paired serum and saliva samples were analyzed. From this total, 24 samples were obtained from children and 1 from one adult during the course of acute hepatitis A; an additional 18 samples were obtained from health professionals from Adolfo Lutz Institute. The sensitivity to detect anti-HAV IgM was 100% (95%CI: 79.1 to 100.0%), employing saliva as clinical samples. In detecting anti-HAV IgA, the sensitivity was 80.8% (95%CI: 60.0 to 92.7%) and for the total antibodies was 82.1% (95%CI: 62.4 to 93.2%). The specificity was 100% for each. The rate of agreement was high comparing the results of serum and saliva samples for detecting HAV antibodies. We conclude that saliva is an acceptable alternative specimen for diagnosing acute hepatitis A infection, and for screening individuals to receive hepatitis A vaccine or immunoglobulin. A possibilidade de identificar infecções presentes ou passadas utilizando fluidos corpóreos que seriam mais facilmente coletados do que o sangue, principalmente em crianças, facilitaria grandemente a investigação e o acompanhamento de surtos de hepatite A, que ocorrem com muita freqüência em nosso meio. Nosso estudo foi desenvolvido com a finalidade de avaliar a detecção dos anticorpos anti-VHA, da classe IgA, IgM, e anticorpos totais em amostras de saliva, usando amostras de soro como padrão. Foram estudadas 43 amostras pareadas de saliva e de soro, colhidas de 24 crianças e de um adulto durante um surto de hepatite A, e de 18 funcionários do Instituto Adolfo Lutz. Empregando saliva como amostra clínica, a sensibilidade para a detecção de anti-VHA IgM foi de 100,0%, de anti-VHA IgA foi de 80,8% e de anti-VHA total foi de 82,1%. Não houve nenhum resultado falso-positivo, sendo a especificidade de 100%. A concordância foi alta entre os resultados das amostras de saliva e soro na detecção dos anticorpos, indicando que amostras de saliva podem ser utilizadas no diagnóstico de infecção aguda pelo VHA e na seleção de indivíduos para vacinação contra o VHA, para conter surtos. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2000-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30439Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 No. 4 (2000); 197-200 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 Núm. 4 (2000); 197-200 Revista do Instituto de Medicina Tropical de São Paulo; v. 42 n. 4 (2000); 197-200 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30439/32323Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessOBA, Isabel TakanoSPINA, Angela Maria MirandaSARACENI, Cláudia PataraLEMOS, Marcílio FigueiredoSENHORAS, Rita de Cássia Ferreira AndradeMOREIRA, Regina CéliaGRANATO, Celso Francisco Hernandes2012-07-07T09:35:24Zoai:revistas.usp.br:article/30439Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:12.693394Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Detection of hepatitis A antibodies by ELISA using saliva as clinical samples
Detecção de anticorpos anti-VHA em amostras de saliva utilizando teste imuno-enzimático
title Detection of hepatitis A antibodies by ELISA using saliva as clinical samples
spellingShingle Detection of hepatitis A antibodies by ELISA using saliva as clinical samples
OBA, Isabel Takano
Hepatiti
Sal
Antibod
Vacc
title_short Detection of hepatitis A antibodies by ELISA using saliva as clinical samples
title_full Detection of hepatitis A antibodies by ELISA using saliva as clinical samples
title_fullStr Detection of hepatitis A antibodies by ELISA using saliva as clinical samples
title_full_unstemmed Detection of hepatitis A antibodies by ELISA using saliva as clinical samples
title_sort Detection of hepatitis A antibodies by ELISA using saliva as clinical samples
author OBA, Isabel Takano
author_facet OBA, Isabel Takano
SPINA, Angela Maria Miranda
SARACENI, Cláudia Patara
LEMOS, Marcílio Figueiredo
SENHORAS, Rita de Cássia Ferreira Andrade
MOREIRA, Regina Célia
GRANATO, Celso Francisco Hernandes
author_role author
author2 SPINA, Angela Maria Miranda
SARACENI, Cláudia Patara
LEMOS, Marcílio Figueiredo
SENHORAS, Rita de Cássia Ferreira Andrade
MOREIRA, Regina Célia
GRANATO, Celso Francisco Hernandes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv OBA, Isabel Takano
SPINA, Angela Maria Miranda
SARACENI, Cláudia Patara
LEMOS, Marcílio Figueiredo
SENHORAS, Rita de Cássia Ferreira Andrade
MOREIRA, Regina Célia
GRANATO, Celso Francisco Hernandes
dc.subject.por.fl_str_mv Hepatiti
Sal
Antibod
Vacc
topic Hepatiti
Sal
Antibod
Vacc
description The possibility of detecting acute infection and immunity using body fluids that are easier to collect than blood, mainly in children, would facilitate the investigation and follow-up of outbreaks of hepatitis A (HAV). Our study was carried out to evaluate the detection of anti-HAV IgM, IgA and total antibodies in saliva using serum samples as reference. Forty three paired serum and saliva samples were analyzed. From this total, 24 samples were obtained from children and 1 from one adult during the course of acute hepatitis A; an additional 18 samples were obtained from health professionals from Adolfo Lutz Institute. The sensitivity to detect anti-HAV IgM was 100% (95%CI: 79.1 to 100.0%), employing saliva as clinical samples. In detecting anti-HAV IgA, the sensitivity was 80.8% (95%CI: 60.0 to 92.7%) and for the total antibodies was 82.1% (95%CI: 62.4 to 93.2%). The specificity was 100% for each. The rate of agreement was high comparing the results of serum and saliva samples for detecting HAV antibodies. We conclude that saliva is an acceptable alternative specimen for diagnosing acute hepatitis A infection, and for screening individuals to receive hepatitis A vaccine or immunoglobulin.
publishDate 2000
dc.date.none.fl_str_mv 2000-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30439
url https://www.revistas.usp.br/rimtsp/article/view/30439
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30439/32323
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 No. 4 (2000); 197-200
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 Núm. 4 (2000); 197-200
Revista do Instituto de Medicina Tropical de São Paulo; v. 42 n. 4 (2000); 197-200
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
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reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
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