Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/31069 |
Resumo: | Parvovirus B19 infection was first discovered in 1975 and it is implicated in fetal death from hydrops fetalis the world over. Diagnosis is usually made through histological identification of the intranuclear inclusion in placenta and fetal organs. However, these cells may be scarce or uncharacteristic, making definitive diagnosis difficult. We analyzed histologically placentas and fetal organs from 34 cases of non-immune hydrops fetalis, stained with Hematoxylin and Eosin (HE) and submitted to immunohistochemistry and polymerase chain reaction (PCR). Of 34 tissue samples, two (5.9%) presented typical intranuclear inclusion in circulating normoblasts seen in Hematoxylin and Eosin stained sections, confirmed by immunohistochemistry and PCR. However, PCR of fetal organs was negative in one case in which the placenta PCR was positive. We concluded that parvovirus B19 infection frequency is similar to the literature and that immunohistochemistry was the best detection method. It is highly specific and sensitive, preserves the morphology and reveals a larger number of positive cells than does HE with the advantage of showing cytoplasmic and nuclear positivity, making it more reliable. Although PCR is more specific and sensitive in fresh or ideally fixed material it is not so in formalin-fixed paraffin-embedded tissues, frequently the only one available in such cases. |
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Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues Detecção da infecção pelo parvovírus B19 em placenta e tecidos fetais fixados em formalina e embebidos em parafina ImmunohistochemistryHydrops fetalisParvovirus B19 Parvovirus B19 infection was first discovered in 1975 and it is implicated in fetal death from hydrops fetalis the world over. Diagnosis is usually made through histological identification of the intranuclear inclusion in placenta and fetal organs. However, these cells may be scarce or uncharacteristic, making definitive diagnosis difficult. We analyzed histologically placentas and fetal organs from 34 cases of non-immune hydrops fetalis, stained with Hematoxylin and Eosin (HE) and submitted to immunohistochemistry and polymerase chain reaction (PCR). Of 34 tissue samples, two (5.9%) presented typical intranuclear inclusion in circulating normoblasts seen in Hematoxylin and Eosin stained sections, confirmed by immunohistochemistry and PCR. However, PCR of fetal organs was negative in one case in which the placenta PCR was positive. We concluded that parvovirus B19 infection frequency is similar to the literature and that immunohistochemistry was the best detection method. It is highly specific and sensitive, preserves the morphology and reveals a larger number of positive cells than does HE with the advantage of showing cytoplasmic and nuclear positivity, making it more reliable. Although PCR is more specific and sensitive in fresh or ideally fixed material it is not so in formalin-fixed paraffin-embedded tissues, frequently the only one available in such cases. O parvovírus B19 foi detectado em 1975 e desde sua descoberta tem se mostrado um agente infeccioso importante em seres humanos, cujo diagnóstico pode ser feito pelo exame histológico através do encontro de inclusão nuclear em tecidos fetais ou placentários. No entanto, estas células podem ser escassas ou não apresentarem características típicas, dificultando o diagnóstico. Analisamos placentas e órgãos fetais de 34 casos de hidropisia fetal não-imune corados com Hematoxilina e Eosina (HE) e submetidos à reação em cadeia da polimerase (PCR) e imuno-histoquímica (IH). Em dois casos (5,9%) houve positividade na placenta pelo HE, IH e PCR. No entanto, PCR dos órgãos fetais foi negativa em um destes casos enquanto que a identificação pôde ser feita por IH e histologia. Concluímos que a freqüência do parvovírus B19 é similar à literatura e a reação IH foi o melhor método de detecção, com identificação mais específica e segura, permitindo identificação citoplasmática, o que não é possível pelo exame histopatológico. A PCR pode apresentar falsa negatividade, provavelmente pela fixação, não identifica as células e é mais dispendiosa. Embora mais específica e sensível em material a fresco ou idealmente fixado isto não ocorre com tecidos fixados em formalina e embebidos em parafina, freqüentemente os únicos disponíveis. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2007-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/31069Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 No. 2 (2007); 103-107 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 Núm. 2 (2007); 103-107 Revista do Instituto de Medicina Tropical de São Paulo; v. 49 n. 2 (2007); 103-107 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/31069/32953Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessQuemelo, Paulo Roberto VeigaLima, Danielle MaltaFonseca, Benedito Antônio Lopes daPeres, Luiz Cesar2012-07-07T19:00:17Zoai:revistas.usp.br:article/31069Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:46.215913Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues Detecção da infecção pelo parvovírus B19 em placenta e tecidos fetais fixados em formalina e embebidos em parafina |
title |
Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues |
spellingShingle |
Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues Quemelo, Paulo Roberto Veiga Immunohistochemistry Hydrops fetalis Parvovirus B19 |
title_short |
Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues |
title_full |
Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues |
title_fullStr |
Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues |
title_full_unstemmed |
Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues |
title_sort |
Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues |
author |
Quemelo, Paulo Roberto Veiga |
author_facet |
Quemelo, Paulo Roberto Veiga Lima, Danielle Malta Fonseca, Benedito Antônio Lopes da Peres, Luiz Cesar |
author_role |
author |
author2 |
Lima, Danielle Malta Fonseca, Benedito Antônio Lopes da Peres, Luiz Cesar |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Quemelo, Paulo Roberto Veiga Lima, Danielle Malta Fonseca, Benedito Antônio Lopes da Peres, Luiz Cesar |
dc.subject.por.fl_str_mv |
Immunohistochemistry Hydrops fetalis Parvovirus B19 |
topic |
Immunohistochemistry Hydrops fetalis Parvovirus B19 |
description |
Parvovirus B19 infection was first discovered in 1975 and it is implicated in fetal death from hydrops fetalis the world over. Diagnosis is usually made through histological identification of the intranuclear inclusion in placenta and fetal organs. However, these cells may be scarce or uncharacteristic, making definitive diagnosis difficult. We analyzed histologically placentas and fetal organs from 34 cases of non-immune hydrops fetalis, stained with Hematoxylin and Eosin (HE) and submitted to immunohistochemistry and polymerase chain reaction (PCR). Of 34 tissue samples, two (5.9%) presented typical intranuclear inclusion in circulating normoblasts seen in Hematoxylin and Eosin stained sections, confirmed by immunohistochemistry and PCR. However, PCR of fetal organs was negative in one case in which the placenta PCR was positive. We concluded that parvovirus B19 infection frequency is similar to the literature and that immunohistochemistry was the best detection method. It is highly specific and sensitive, preserves the morphology and reveals a larger number of positive cells than does HE with the advantage of showing cytoplasmic and nuclear positivity, making it more reliable. Although PCR is more specific and sensitive in fresh or ideally fixed material it is not so in formalin-fixed paraffin-embedded tissues, frequently the only one available in such cases. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31069 |
url |
https://www.revistas.usp.br/rimtsp/article/view/31069 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/31069/32953 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 No. 2 (2007); 103-107 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 49 Núm. 2 (2007); 103-107 Revista do Instituto de Medicina Tropical de São Paulo; v. 49 n. 2 (2007); 103-107 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951646208720896 |