Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis

Detalhes bibliográficos
Autor(a) principal: Vidal, Mônica Scarpelli Martinelli
Data de Publicação: 2003
Outros Autores: Castro, Luis Guilherme Martins de, Cavalecate, Sônia Cristina, Lacaz, Carlos da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30761
Resumo: Chromoblastomycosis (CBM) is a chronic subcutaneous infection caused by several dematiaceous fungi. The most commonly etiological agent found in Brazil is Fonsecaea pedrosoi, which appears as thick walled, brownish colored cells with transverse and longitudinal division in the lesions, called "muriform cells". This disease is found worldwide but countries like Madagascar and Brazil have highest incidence. Diagnosis is made by clinical, direct and histopathologic examination and culture of specimens. Serological tests have been used to identify specific antibodies against Fonsecaea pedrosoi antigens, as well as immunotechniques have been used for CBM serological identification and diagnosis. In the present study double immunodiffusion (DID), counterimmunoelectrophoresis (CIE) and immunoenzymatic test (ELISA) have been used to evaluate humoral immune response in patients with CBM caused by F. pedrosoi. Metabolic antigen was used for immunoprecipitation tests (DID and CIE) while somatic antigen for ELISA. Our results demonstrated 53% sensitivity and 96% specificity for DID, while CIE presented 68% sensitivity and 90.5% specificity. ELISA demonstrated 78% sensibility and 83% specificity. Serological tests can be a useful tool to study different aspects of CBM, such as helping differential diagnosis, when culture of the pathogenic agent is impossible.
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spelling Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis Técnicas de imunoprecipitação e Elisa na detecção de anticorpos anti-Fonsecaea pedrosoi na cromoblastomicose ChromoblastomycosisFonsecaea pedrosoiSerologic tests and antigen Chromoblastomycosis (CBM) is a chronic subcutaneous infection caused by several dematiaceous fungi. The most commonly etiological agent found in Brazil is Fonsecaea pedrosoi, which appears as thick walled, brownish colored cells with transverse and longitudinal division in the lesions, called "muriform cells". This disease is found worldwide but countries like Madagascar and Brazil have highest incidence. Diagnosis is made by clinical, direct and histopathologic examination and culture of specimens. Serological tests have been used to identify specific antibodies against Fonsecaea pedrosoi antigens, as well as immunotechniques have been used for CBM serological identification and diagnosis. In the present study double immunodiffusion (DID), counterimmunoelectrophoresis (CIE) and immunoenzymatic test (ELISA) have been used to evaluate humoral immune response in patients with CBM caused by F. pedrosoi. Metabolic antigen was used for immunoprecipitation tests (DID and CIE) while somatic antigen for ELISA. Our results demonstrated 53% sensitivity and 96% specificity for DID, while CIE presented 68% sensitivity and 90.5% specificity. ELISA demonstrated 78% sensibility and 83% specificity. Serological tests can be a useful tool to study different aspects of CBM, such as helping differential diagnosis, when culture of the pathogenic agent is impossible. Cromoblastomicose (CBM) é infecção subcutânea causada por vários fungos demáceos. O agente mais importante no Brasil é Fonsecaea pedrosoi, que se apresenta nas lesões como células de coloração acastanhada, com divisão transversal e longitudinal, originando as denominadas "células muriformes". Esta infecção apresenta caráter universal, mas países como Madagascar e Brasil apresentam alta incidência. O diagnóstico é realizado através dos exames clínico, direto e histopatológico, acompanhado de cultura e identificação do agente etiológico. Os testes sorológicos foram aplicados para identificar anticorpos específicos frente a antígenos de Fonsecaea pedrosoi e várias metodologias têm sido empregadas para identificação sorológica e o diagnóstico da CBM. Neste estudo, avaliamos reações de imunodifusão dupla (DID), contraimunoeletroforese (CIE) e teste imunoenzimático (ELISA) para avaliar a resposta imune humoral na CBM causada por F. pedrosoi. Utilizamos antígeno metabólico para DID e CIE e antígeno somático para ELISA. Nossos dados revelaram 53% e 68% de sensibilidade e 96% e 90,5% de especificidade, respectivamente. O teste de ELISA demonstrou 78% de sensibilidade e 83% de especificidade. Estes resultados indicam que as reações sorológicas podem ser uma ferramenta útil no auxílio diagnóstico desta infecção, quando a cultura do agente não for possível. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30761Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 6 (2003); 315-318 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 6 (2003); 315-318 Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 6 (2003); 315-318 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30761/32645Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessVidal, Mônica Scarpelli MartinelliCastro, Luis Guilherme Martins deCavalecate, Sônia CristinaLacaz, Carlos da Silva2012-07-07T18:06:54Zoai:revistas.usp.br:article/30761Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:31.024996Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis
Técnicas de imunoprecipitação e Elisa na detecção de anticorpos anti-Fonsecaea pedrosoi na cromoblastomicose
title Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis
spellingShingle Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis
Vidal, Mônica Scarpelli Martinelli
Chromoblastomycosis
Fonsecaea pedrosoi
Serologic tests and antigen
title_short Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis
title_full Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis
title_fullStr Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis
title_full_unstemmed Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis
title_sort Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis
author Vidal, Mônica Scarpelli Martinelli
author_facet Vidal, Mônica Scarpelli Martinelli
Castro, Luis Guilherme Martins de
Cavalecate, Sônia Cristina
Lacaz, Carlos da Silva
author_role author
author2 Castro, Luis Guilherme Martins de
Cavalecate, Sônia Cristina
Lacaz, Carlos da Silva
author2_role author
author
author
dc.contributor.author.fl_str_mv Vidal, Mônica Scarpelli Martinelli
Castro, Luis Guilherme Martins de
Cavalecate, Sônia Cristina
Lacaz, Carlos da Silva
dc.subject.por.fl_str_mv Chromoblastomycosis
Fonsecaea pedrosoi
Serologic tests and antigen
topic Chromoblastomycosis
Fonsecaea pedrosoi
Serologic tests and antigen
description Chromoblastomycosis (CBM) is a chronic subcutaneous infection caused by several dematiaceous fungi. The most commonly etiological agent found in Brazil is Fonsecaea pedrosoi, which appears as thick walled, brownish colored cells with transverse and longitudinal division in the lesions, called "muriform cells". This disease is found worldwide but countries like Madagascar and Brazil have highest incidence. Diagnosis is made by clinical, direct and histopathologic examination and culture of specimens. Serological tests have been used to identify specific antibodies against Fonsecaea pedrosoi antigens, as well as immunotechniques have been used for CBM serological identification and diagnosis. In the present study double immunodiffusion (DID), counterimmunoelectrophoresis (CIE) and immunoenzymatic test (ELISA) have been used to evaluate humoral immune response in patients with CBM caused by F. pedrosoi. Metabolic antigen was used for immunoprecipitation tests (DID and CIE) while somatic antigen for ELISA. Our results demonstrated 53% sensitivity and 96% specificity for DID, while CIE presented 68% sensitivity and 90.5% specificity. ELISA demonstrated 78% sensibility and 83% specificity. Serological tests can be a useful tool to study different aspects of CBM, such as helping differential diagnosis, when culture of the pathogenic agent is impossible.
publishDate 2003
dc.date.none.fl_str_mv 2003-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30761
url https://www.revistas.usp.br/rimtsp/article/view/30761
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30761/32645
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 6 (2003); 315-318
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 6 (2003); 315-318
Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 6 (2003); 315-318
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
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instname_str Instituto de Medicina Tropical (IMT)
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reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
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