Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30761 |
Resumo: | Chromoblastomycosis (CBM) is a chronic subcutaneous infection caused by several dematiaceous fungi. The most commonly etiological agent found in Brazil is Fonsecaea pedrosoi, which appears as thick walled, brownish colored cells with transverse and longitudinal division in the lesions, called "muriform cells". This disease is found worldwide but countries like Madagascar and Brazil have highest incidence. Diagnosis is made by clinical, direct and histopathologic examination and culture of specimens. Serological tests have been used to identify specific antibodies against Fonsecaea pedrosoi antigens, as well as immunotechniques have been used for CBM serological identification and diagnosis. In the present study double immunodiffusion (DID), counterimmunoelectrophoresis (CIE) and immunoenzymatic test (ELISA) have been used to evaluate humoral immune response in patients with CBM caused by F. pedrosoi. Metabolic antigen was used for immunoprecipitation tests (DID and CIE) while somatic antigen for ELISA. Our results demonstrated 53% sensitivity and 96% specificity for DID, while CIE presented 68% sensitivity and 90.5% specificity. ELISA demonstrated 78% sensibility and 83% specificity. Serological tests can be a useful tool to study different aspects of CBM, such as helping differential diagnosis, when culture of the pathogenic agent is impossible. |
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Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis Técnicas de imunoprecipitação e Elisa na detecção de anticorpos anti-Fonsecaea pedrosoi na cromoblastomicose ChromoblastomycosisFonsecaea pedrosoiSerologic tests and antigen Chromoblastomycosis (CBM) is a chronic subcutaneous infection caused by several dematiaceous fungi. The most commonly etiological agent found in Brazil is Fonsecaea pedrosoi, which appears as thick walled, brownish colored cells with transverse and longitudinal division in the lesions, called "muriform cells". This disease is found worldwide but countries like Madagascar and Brazil have highest incidence. Diagnosis is made by clinical, direct and histopathologic examination and culture of specimens. Serological tests have been used to identify specific antibodies against Fonsecaea pedrosoi antigens, as well as immunotechniques have been used for CBM serological identification and diagnosis. In the present study double immunodiffusion (DID), counterimmunoelectrophoresis (CIE) and immunoenzymatic test (ELISA) have been used to evaluate humoral immune response in patients with CBM caused by F. pedrosoi. Metabolic antigen was used for immunoprecipitation tests (DID and CIE) while somatic antigen for ELISA. Our results demonstrated 53% sensitivity and 96% specificity for DID, while CIE presented 68% sensitivity and 90.5% specificity. ELISA demonstrated 78% sensibility and 83% specificity. Serological tests can be a useful tool to study different aspects of CBM, such as helping differential diagnosis, when culture of the pathogenic agent is impossible. Cromoblastomicose (CBM) é infecção subcutânea causada por vários fungos demáceos. O agente mais importante no Brasil é Fonsecaea pedrosoi, que se apresenta nas lesões como células de coloração acastanhada, com divisão transversal e longitudinal, originando as denominadas "células muriformes". Esta infecção apresenta caráter universal, mas países como Madagascar e Brasil apresentam alta incidência. O diagnóstico é realizado através dos exames clínico, direto e histopatológico, acompanhado de cultura e identificação do agente etiológico. Os testes sorológicos foram aplicados para identificar anticorpos específicos frente a antígenos de Fonsecaea pedrosoi e várias metodologias têm sido empregadas para identificação sorológica e o diagnóstico da CBM. Neste estudo, avaliamos reações de imunodifusão dupla (DID), contraimunoeletroforese (CIE) e teste imunoenzimático (ELISA) para avaliar a resposta imune humoral na CBM causada por F. pedrosoi. Utilizamos antígeno metabólico para DID e CIE e antígeno somático para ELISA. Nossos dados revelaram 53% e 68% de sensibilidade e 96% e 90,5% de especificidade, respectivamente. O teste de ELISA demonstrou 78% de sensibilidade e 83% de especificidade. Estes resultados indicam que as reações sorológicas podem ser uma ferramenta útil no auxílio diagnóstico desta infecção, quando a cultura do agente não for possível. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30761Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 6 (2003); 315-318 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 6 (2003); 315-318 Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 6 (2003); 315-318 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30761/32645Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessVidal, Mônica Scarpelli MartinelliCastro, Luis Guilherme Martins deCavalecate, Sônia CristinaLacaz, Carlos da Silva2012-07-07T18:06:54Zoai:revistas.usp.br:article/30761Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:31.024996Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis Técnicas de imunoprecipitação e Elisa na detecção de anticorpos anti-Fonsecaea pedrosoi na cromoblastomicose |
title |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis |
spellingShingle |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis Vidal, Mônica Scarpelli Martinelli Chromoblastomycosis Fonsecaea pedrosoi Serologic tests and antigen |
title_short |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis |
title_full |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis |
title_fullStr |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis |
title_full_unstemmed |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis |
title_sort |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis |
author |
Vidal, Mônica Scarpelli Martinelli |
author_facet |
Vidal, Mônica Scarpelli Martinelli Castro, Luis Guilherme Martins de Cavalecate, Sônia Cristina Lacaz, Carlos da Silva |
author_role |
author |
author2 |
Castro, Luis Guilherme Martins de Cavalecate, Sônia Cristina Lacaz, Carlos da Silva |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Vidal, Mônica Scarpelli Martinelli Castro, Luis Guilherme Martins de Cavalecate, Sônia Cristina Lacaz, Carlos da Silva |
dc.subject.por.fl_str_mv |
Chromoblastomycosis Fonsecaea pedrosoi Serologic tests and antigen |
topic |
Chromoblastomycosis Fonsecaea pedrosoi Serologic tests and antigen |
description |
Chromoblastomycosis (CBM) is a chronic subcutaneous infection caused by several dematiaceous fungi. The most commonly etiological agent found in Brazil is Fonsecaea pedrosoi, which appears as thick walled, brownish colored cells with transverse and longitudinal division in the lesions, called "muriform cells". This disease is found worldwide but countries like Madagascar and Brazil have highest incidence. Diagnosis is made by clinical, direct and histopathologic examination and culture of specimens. Serological tests have been used to identify specific antibodies against Fonsecaea pedrosoi antigens, as well as immunotechniques have been used for CBM serological identification and diagnosis. In the present study double immunodiffusion (DID), counterimmunoelectrophoresis (CIE) and immunoenzymatic test (ELISA) have been used to evaluate humoral immune response in patients with CBM caused by F. pedrosoi. Metabolic antigen was used for immunoprecipitation tests (DID and CIE) while somatic antigen for ELISA. Our results demonstrated 53% sensitivity and 96% specificity for DID, while CIE presented 68% sensitivity and 90.5% specificity. ELISA demonstrated 78% sensibility and 83% specificity. Serological tests can be a useful tool to study different aspects of CBM, such as helping differential diagnosis, when culture of the pathogenic agent is impossible. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30761 |
url |
https://www.revistas.usp.br/rimtsp/article/view/30761 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30761/32645 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 6 (2003); 315-318 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 6 (2003); 315-318 Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 6 (2003); 315-318 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951644475424768 |