Immunofluorescence assay reactivity patterns of serum samples presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/30546 |
Resumo: | Serum samples (n: 110) from blood donors and high risk individuals from Cordoba, Argentina with indeterminate HIV-1 and HTLV-I/II Wb profiles were studied for specific antibodies to HTLV-I/II and HIV-1 by indirect immunofluorescence assay (IFA) and for the presence or absence of HIV-1 and HTLV-I/II specific bands by Wb. This study was carried out in order to characterize their putative reactions with HIV-1 and HTLV-I/II proteins and to resolve the retrovirus infection status of these individuals. Results indicated that blood donors sera displaying indeterminate HIV-1 or HTLV-I/II Wb patterns were not immunoreactive to HTLV-I/II and HIV-1 on IFA. However, a high rate of indeterminate HIV-1 and HTLV-I/II Wb samples from high risk individuals had positive HTLV-I/II and HIV-1 IFA results respectively. Our study supports the growing evidence that HTLV-HIV indeterminate seroreactivity in low risk population is due to a cross reaction against nonviral antigens, and in high risk populations the indeterminate samples show serological cross-recognition between HIV-1 proteins and HTLV-I/II proteins on Wb. These results point out the necessity to investigate the HTLV-I/II reactivity in indeterminate HIV-1 samples and viceversa in order to confirm the diagnosis. Finally, this study shows the potential usefulness of IFA in elucidating the status of HIV-1 and HTLV-I/II infection of individuals with indeterminate Wb profiles, thus enabling resolution of retrovirus infection status. |
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Immunofluorescence assay reactivity patterns of serum samples presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina Parâmetros de reatividade de amostras de soro com resultados indeterminados por Western blot para anticorpos contra HIV-1 e HTLV I/II em Córdoba, Argentina RetrovirusesHIV-1HTLV-I/IIConfirmatory testingIndeterminate resultsWestern blotIndirect immunofluorescence assay Serum samples (n: 110) from blood donors and high risk individuals from Cordoba, Argentina with indeterminate HIV-1 and HTLV-I/II Wb profiles were studied for specific antibodies to HTLV-I/II and HIV-1 by indirect immunofluorescence assay (IFA) and for the presence or absence of HIV-1 and HTLV-I/II specific bands by Wb. This study was carried out in order to characterize their putative reactions with HIV-1 and HTLV-I/II proteins and to resolve the retrovirus infection status of these individuals. Results indicated that blood donors sera displaying indeterminate HIV-1 or HTLV-I/II Wb patterns were not immunoreactive to HTLV-I/II and HIV-1 on IFA. However, a high rate of indeterminate HIV-1 and HTLV-I/II Wb samples from high risk individuals had positive HTLV-I/II and HIV-1 IFA results respectively. Our study supports the growing evidence that HTLV-HIV indeterminate seroreactivity in low risk population is due to a cross reaction against nonviral antigens, and in high risk populations the indeterminate samples show serological cross-recognition between HIV-1 proteins and HTLV-I/II proteins on Wb. These results point out the necessity to investigate the HTLV-I/II reactivity in indeterminate HIV-1 samples and viceversa in order to confirm the diagnosis. Finally, this study shows the potential usefulness of IFA in elucidating the status of HIV-1 and HTLV-I/II infection of individuals with indeterminate Wb profiles, thus enabling resolution of retrovirus infection status. Amostras de soro sangüíneo (n: 110) de indivíduos de comportamento de risco e doadores de sangue da cidade de Córdoba, na Argentina, com perfis de reactividade para HIV-1 e HTLV-I/II indeterminada por Western blot (Wb), foram estudadas para anticorpos específicos contra HTLV-I/II e HIV-1 por meio do Ensaio de Imunofluorescência Indireta (IFI). Este estudo foi realizado para caracterizar as reações putativas com proteínas HIV-1 e HTLV-I/II e resolver o estado da infecção por retrovírus destes indivíduos. Os resultados mostram que os soros dos doadores sangüíneos que apresentam padrões indeterminados para HTLV-I/II e HIV-1 no Wb não são reagentes contra HTLV-I/II e HIV-1 por IFI. Mas, um alto índice de amostras de individuos com alto risco com resultado indeterminado no Wb para HIV-1 e HTLV-I/II apresentaram resultados positivos para HTLV-I/II e HIV-1 por IFI, respectivamente. Nosso estudo sugere que a reatividade indeterminada para HTLV-HIV na população de baixo risco deve-se a uma reação cruzada contra antígenos não virais; e que na população de alto risco as amostras indeterminadas apresentam reação cruzada entre as proteínas HIV-1 e HTLV-I/II no Wb. Estes resultados indicam que se faz preciso pesquisar a reatividade de HTLV-I/II nas amostras indeterminadas de HIV-1 e vice-versa, para confirmar o diagnóstico. Por último, este trabalho mostra a utilidade potencial da IFI para determinar o estado de infecção HIV-1 e HTLV-I/II dos indivíduos com perfis indeterminados por Wb, permitindo assim, a resolução do estado real de infecção por retrovírus. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2001-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30546Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 5 (2001); 277-282 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 5 (2001); 277-282 Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 5 (2001); 277-282 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30546/32430Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessGASTALDELLO, RenéGALLEGO, SandraISA, María BeatrizMATURANO, EduardoSILEONI, SantosNATES, SilviaMEDEOT, Silvia2012-07-07T13:35:32Zoai:revistas.usp.br:article/30546Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:19.902838Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Immunofluorescence assay reactivity patterns of serum samples presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina Parâmetros de reatividade de amostras de soro com resultados indeterminados por Western blot para anticorpos contra HIV-1 e HTLV I/II em Córdoba, Argentina |
title |
Immunofluorescence assay reactivity patterns of serum samples presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina |
spellingShingle |
Immunofluorescence assay reactivity patterns of serum samples presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina GASTALDELLO, René Retroviruses HIV-1 HTLV-I/II Confirmatory testing Indeterminate results Western blot Indirect immunofluorescence assay |
title_short |
Immunofluorescence assay reactivity patterns of serum samples presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina |
title_full |
Immunofluorescence assay reactivity patterns of serum samples presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina |
title_fullStr |
Immunofluorescence assay reactivity patterns of serum samples presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina |
title_full_unstemmed |
Immunofluorescence assay reactivity patterns of serum samples presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina |
title_sort |
Immunofluorescence assay reactivity patterns of serum samples presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina |
author |
GASTALDELLO, René |
author_facet |
GASTALDELLO, René GALLEGO, Sandra ISA, María Beatriz MATURANO, Eduardo SILEONI, Santos NATES, Silvia MEDEOT, Silvia |
author_role |
author |
author2 |
GALLEGO, Sandra ISA, María Beatriz MATURANO, Eduardo SILEONI, Santos NATES, Silvia MEDEOT, Silvia |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
GASTALDELLO, René GALLEGO, Sandra ISA, María Beatriz MATURANO, Eduardo SILEONI, Santos NATES, Silvia MEDEOT, Silvia |
dc.subject.por.fl_str_mv |
Retroviruses HIV-1 HTLV-I/II Confirmatory testing Indeterminate results Western blot Indirect immunofluorescence assay |
topic |
Retroviruses HIV-1 HTLV-I/II Confirmatory testing Indeterminate results Western blot Indirect immunofluorescence assay |
description |
Serum samples (n: 110) from blood donors and high risk individuals from Cordoba, Argentina with indeterminate HIV-1 and HTLV-I/II Wb profiles were studied for specific antibodies to HTLV-I/II and HIV-1 by indirect immunofluorescence assay (IFA) and for the presence or absence of HIV-1 and HTLV-I/II specific bands by Wb. This study was carried out in order to characterize their putative reactions with HIV-1 and HTLV-I/II proteins and to resolve the retrovirus infection status of these individuals. Results indicated that blood donors sera displaying indeterminate HIV-1 or HTLV-I/II Wb patterns were not immunoreactive to HTLV-I/II and HIV-1 on IFA. However, a high rate of indeterminate HIV-1 and HTLV-I/II Wb samples from high risk individuals had positive HTLV-I/II and HIV-1 IFA results respectively. Our study supports the growing evidence that HTLV-HIV indeterminate seroreactivity in low risk population is due to a cross reaction against nonviral antigens, and in high risk populations the indeterminate samples show serological cross-recognition between HIV-1 proteins and HTLV-I/II proteins on Wb. These results point out the necessity to investigate the HTLV-I/II reactivity in indeterminate HIV-1 samples and viceversa in order to confirm the diagnosis. Finally, this study shows the potential usefulness of IFA in elucidating the status of HIV-1 and HTLV-I/II infection of individuals with indeterminate Wb profiles, thus enabling resolution of retrovirus infection status. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30546 |
url |
https://www.revistas.usp.br/rimtsp/article/view/30546 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/30546/32430 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 5 (2001); 277-282 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 5 (2001); 277-282 Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 5 (2001); 277-282 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
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Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
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Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951643329331200 |