Spotted fever: Rickettsia isolation in skin biopsy sample

Detalhes bibliográficos
Autor(a) principal: Melles, Heloisa Helana B.
Data de Publicação: 1992
Outros Autores: Colombo, Silvia, Silva, Marcos Vinícius da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28889
Resumo: A 2 years old child living in an area of the State of São Paulo, known in the past as endemic for rickettsiosis developed clinical evidences of spotted fever after a tick bite. Rickettsiae were isolated from guinea pigs inoculated with a skin homogenate. In sera tested by indirect immunofluorescence with Rickettsia rickettsii standard antigen, IgG specific antibody titers raised from 1:512 in the first sample to 1:2048 in the third one; IgM specific antibody titer was 1:128 in the three samples. Also positive were sera obtained from the inoculated guinea pigs. In the last 20 years no other case of rickettsial spotted fever has been confirmed by isolation of the agent in Brasil. To our knowlwdge, there are no previous reports of isolation of Rickettsiae through inoculation of skin biopsy homogenates.
id IMT-1_98a7a4a81449a140df6b2fe5cf6dea66
oai_identifier_str oai:revistas.usp.br:article/28889
network_acronym_str IMT-1
network_name_str Revista do Instituto de Medicina Tropical de São Paulo
repository_id_str
spelling Spotted fever: Rickettsia isolation in skin biopsy sample Febre maculosa: isolamento de Rickettsia em amostra de biópsia de pele Febre MaculosaRickettsia rickettsiiRickettsiose-sorologiaRickettsiose-cultivo A 2 years old child living in an area of the State of São Paulo, known in the past as endemic for rickettsiosis developed clinical evidences of spotted fever after a tick bite. Rickettsiae were isolated from guinea pigs inoculated with a skin homogenate. In sera tested by indirect immunofluorescence with Rickettsia rickettsii standard antigen, IgG specific antibody titers raised from 1:512 in the first sample to 1:2048 in the third one; IgM specific antibody titer was 1:128 in the three samples. Also positive were sera obtained from the inoculated guinea pigs. In the last 20 years no other case of rickettsial spotted fever has been confirmed by isolation of the agent in Brasil. To our knowlwdge, there are no previous reports of isolation of Rickettsiae through inoculation of skin biopsy homogenates. Presença de Rickettsia na pele de doente de Febre Maculosa foi evidenciada por inoculação intraperitoneal em cobaio. O diagnóstico sorológico por imunoflüorescência indireta revelou diferença de título de anticorpos específicos para Rickettsia rickettsii, de 4 vezes entre a 1º e a 3º amostra. Imunoglobulina M (IgM) específica foi detectada nas amostras de sangue, evidência de infecção em atividade ou recente. Foi também detectada a presença de anticorpos específicos para R. rickettsii no soro dos cobaios inoculados. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1992-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28889Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 1 (1992); 37-41 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 1 (1992); 37-41 Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 1 (1992); 37-41 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/28889/30743Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessMelles, Heloisa Helana B.Colombo, SilviaSilva, Marcos Vinícius da2012-07-02T01:32:24Zoai:revistas.usp.br:article/28889Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:39.238026Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Spotted fever: Rickettsia isolation in skin biopsy sample
Febre maculosa: isolamento de Rickettsia em amostra de biópsia de pele
title Spotted fever: Rickettsia isolation in skin biopsy sample
spellingShingle Spotted fever: Rickettsia isolation in skin biopsy sample
Melles, Heloisa Helana B.
Febre Maculosa
Rickettsia rickettsii
Rickettsiose-sorologia
Rickettsiose-cultivo
title_short Spotted fever: Rickettsia isolation in skin biopsy sample
title_full Spotted fever: Rickettsia isolation in skin biopsy sample
title_fullStr Spotted fever: Rickettsia isolation in skin biopsy sample
title_full_unstemmed Spotted fever: Rickettsia isolation in skin biopsy sample
title_sort Spotted fever: Rickettsia isolation in skin biopsy sample
author Melles, Heloisa Helana B.
author_facet Melles, Heloisa Helana B.
Colombo, Silvia
Silva, Marcos Vinícius da
author_role author
author2 Colombo, Silvia
Silva, Marcos Vinícius da
author2_role author
author
dc.contributor.author.fl_str_mv Melles, Heloisa Helana B.
Colombo, Silvia
Silva, Marcos Vinícius da
dc.subject.por.fl_str_mv Febre Maculosa
Rickettsia rickettsii
Rickettsiose-sorologia
Rickettsiose-cultivo
topic Febre Maculosa
Rickettsia rickettsii
Rickettsiose-sorologia
Rickettsiose-cultivo
description A 2 years old child living in an area of the State of São Paulo, known in the past as endemic for rickettsiosis developed clinical evidences of spotted fever after a tick bite. Rickettsiae were isolated from guinea pigs inoculated with a skin homogenate. In sera tested by indirect immunofluorescence with Rickettsia rickettsii standard antigen, IgG specific antibody titers raised from 1:512 in the first sample to 1:2048 in the third one; IgM specific antibody titer was 1:128 in the three samples. Also positive were sera obtained from the inoculated guinea pigs. In the last 20 years no other case of rickettsial spotted fever has been confirmed by isolation of the agent in Brasil. To our knowlwdge, there are no previous reports of isolation of Rickettsiae through inoculation of skin biopsy homogenates.
publishDate 1992
dc.date.none.fl_str_mv 1992-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28889
url https://www.revistas.usp.br/rimtsp/article/view/28889
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28889/30743
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 1 (1992); 37-41
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 1 (1992); 37-41
Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 1 (1992); 37-41
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
_version_ 1798951639381442560

Registros relacionados