The use of aminoacids in the study of Paracoccidioides brasiliensis growth and its role in fungus dimorphism
Autor(a) principal: | |
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Data de Publicação: | 1991 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/28842 |
Resumo: | Fiftenn Paracoccidioides brasiliensis strains, in the mycelial (M) and yeast like (Y), were cultivated in minimal medium (MM) and subcultivated to be adapted to the same medium supplemented with a pool of amminoacid in solution (MMS). Each of the aminoacids were studied separately of the solution to provide the auxological study. The prototrophism was demonstrated by nine strains in both M and Y forms, and the auxotrofism by the remaining strains. The heterologous results has not allowed us to draw an auxological characterization of the P. brasiliensis. As far as we could observe none of the aminoacid studied in this piece of research can be considered of absolute importance for to the growth and the morphogenesis maintainance of the fungus. Morphological alterations were only verified in the prototroph strains, which suggest that there could have been adaptative metabolism activity due to the absence of organic nitrogen compounds in the minimal medium (MM). |
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The use of aminoacids in the study of Paracoccidioides brasiliensis growth and its role in fungus dimorphism Utilização de aminoácidos no estudo do crescimento do Paracoccidioides brasiliensis: Influência sobre o dimorfismo Paracoccidioides brasiliensisAminoácidosDimorfismo Fiftenn Paracoccidioides brasiliensis strains, in the mycelial (M) and yeast like (Y), were cultivated in minimal medium (MM) and subcultivated to be adapted to the same medium supplemented with a pool of amminoacid in solution (MMS). Each of the aminoacids were studied separately of the solution to provide the auxological study. The prototrophism was demonstrated by nine strains in both M and Y forms, and the auxotrofism by the remaining strains. The heterologous results has not allowed us to draw an auxological characterization of the P. brasiliensis. As far as we could observe none of the aminoacid studied in this piece of research can be considered of absolute importance for to the growth and the morphogenesis maintainance of the fungus. Morphological alterations were only verified in the prototroph strains, which suggest that there could have been adaptative metabolism activity due to the absence of organic nitrogen compounds in the minimal medium (MM). Utilizamos 15 amostras de Paracoccidioides brasiliensis nas formas miceliana (M) e leveduriforme (L), cultivadas em meio mínimo (MM) e adaptadas ao mesmo meio suplementado com a solução de aminoácidos (MMS). Para a realização do estudo auxológico das amostras, foram preparadas soluções complementares das quais foram retirados um aminoácido de cada vez. Nove amostras foram prototróficas nas formas M e/ou L e as demais auxotróficas para os diferentes aminoácidos e bases nitrogenadas. A heterogeneidade dos resultados apresentados não permitiu a caracterização auxológica das 15 amostras de P. brasiliensis estudadas. Nenhum dos compostos nitrogenados demonstrou ser essencial para o crescimento ou para a manutenção da morfogênese do fungo. Alterações morfológicas (macro e microscópicas) também foram observadas, mas somente entre as amostras prototróficas, sugerindo a ativação de um mecanismo de adaptação desenvolvido pelo fungo mediante a ausência de substratos nitrogenados no meio de cultura (MM). Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1991-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28842Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 4 (1991); 319-324 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 4 (1991); 319-324 Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 4 (1991); 319-324 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/28842/30695Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCano, Maria Isabel NogueiraAguiar, Mônica S.M. Vidal de2012-07-02T01:30:51Zoai:revistas.usp.br:article/28842Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:36.574028Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
The use of aminoacids in the study of Paracoccidioides brasiliensis growth and its role in fungus dimorphism Utilização de aminoácidos no estudo do crescimento do Paracoccidioides brasiliensis: Influência sobre o dimorfismo |
title |
The use of aminoacids in the study of Paracoccidioides brasiliensis growth and its role in fungus dimorphism |
spellingShingle |
The use of aminoacids in the study of Paracoccidioides brasiliensis growth and its role in fungus dimorphism Cano, Maria Isabel Nogueira Paracoccidioides brasiliensis Aminoácidos Dimorfismo |
title_short |
The use of aminoacids in the study of Paracoccidioides brasiliensis growth and its role in fungus dimorphism |
title_full |
The use of aminoacids in the study of Paracoccidioides brasiliensis growth and its role in fungus dimorphism |
title_fullStr |
The use of aminoacids in the study of Paracoccidioides brasiliensis growth and its role in fungus dimorphism |
title_full_unstemmed |
The use of aminoacids in the study of Paracoccidioides brasiliensis growth and its role in fungus dimorphism |
title_sort |
The use of aminoacids in the study of Paracoccidioides brasiliensis growth and its role in fungus dimorphism |
author |
Cano, Maria Isabel Nogueira |
author_facet |
Cano, Maria Isabel Nogueira Aguiar, Mônica S.M. Vidal de |
author_role |
author |
author2 |
Aguiar, Mônica S.M. Vidal de |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Cano, Maria Isabel Nogueira Aguiar, Mônica S.M. Vidal de |
dc.subject.por.fl_str_mv |
Paracoccidioides brasiliensis Aminoácidos Dimorfismo |
topic |
Paracoccidioides brasiliensis Aminoácidos Dimorfismo |
description |
Fiftenn Paracoccidioides brasiliensis strains, in the mycelial (M) and yeast like (Y), were cultivated in minimal medium (MM) and subcultivated to be adapted to the same medium supplemented with a pool of amminoacid in solution (MMS). Each of the aminoacids were studied separately of the solution to provide the auxological study. The prototrophism was demonstrated by nine strains in both M and Y forms, and the auxotrofism by the remaining strains. The heterologous results has not allowed us to draw an auxological characterization of the P. brasiliensis. As far as we could observe none of the aminoacid studied in this piece of research can be considered of absolute importance for to the growth and the morphogenesis maintainance of the fungus. Morphological alterations were only verified in the prototroph strains, which suggest that there could have been adaptative metabolism activity due to the absence of organic nitrogen compounds in the minimal medium (MM). |
publishDate |
1991 |
dc.date.none.fl_str_mv |
1991-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28842 |
url |
https://www.revistas.usp.br/rimtsp/article/view/28842 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/28842/30695 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 4 (1991); 319-324 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 4 (1991); 319-324 Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 4 (1991); 319-324 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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