Evaluation of the double diffusion, enzyme immunoassay and immunoblotting techniques, for the diagnosis of human hydatid disease in tropical areas
Autor(a) principal: | |
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Data de Publicação: | 1994 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/29155 |
Resumo: | Hydatid disease in tropical areas poses a serious diagnostic problem due to the high frequence of cross-reactivity with other endemic helminthic infections. The enzyme-linked-immunosorbent assay (ELISA) and the double diffusion arc 5 showed respectively a sensitivity of 73% and 57% and a specificity of 84-95% and 100%. However, the specificity of ELISA was greatly increased by using ovine serum and phosphorylcholine in the diluent buffer. The hydatic antigen obtained from ovine cyst fluid showed three main protein bands of 64,58 and 30 KDa using SDS PAGE and immunoblotting. Sera from patients with onchocerciasis, cysticercosis, toxocariasis and Strongyloides infection cross-reacted with the 64 and 58 KDa bands by immunoblotting. However, none of the analyzed sera recognized the 30 KDa band, that seems to be specific in this assay. The immunoblotting showed a sensitivity of 80% and a specificity of 100% when used to recognize the 30 KDa band. |
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Evaluation of the double diffusion, enzyme immunoassay and immunoblotting techniques, for the diagnosis of human hydatid disease in tropical areas Evaluación de las técnicas de Doble Difusión, Ensayo Inmunoenzimático e Inmunoblotting en el diagnóstico de la hidatidosis humana en áreas tropicales Hydatid DiseaseCross ReactivityDouble DiffusionELISAImmunoblotting Hydatid disease in tropical areas poses a serious diagnostic problem due to the high frequence of cross-reactivity with other endemic helminthic infections. The enzyme-linked-immunosorbent assay (ELISA) and the double diffusion arc 5 showed respectively a sensitivity of 73% and 57% and a specificity of 84-95% and 100%. However, the specificity of ELISA was greatly increased by using ovine serum and phosphorylcholine in the diluent buffer. The hydatic antigen obtained from ovine cyst fluid showed three main protein bands of 64,58 and 30 KDa using SDS PAGE and immunoblotting. Sera from patients with onchocerciasis, cysticercosis, toxocariasis and Strongyloides infection cross-reacted with the 64 and 58 KDa bands by immunoblotting. However, none of the analyzed sera recognized the 30 KDa band, that seems to be specific in this assay. The immunoblotting showed a sensitivity of 80% and a specificity of 100% when used to recognize the 30 KDa band. La Hidatidosis en áreas tropicales representa un serio problema diagnóstico por la alta frecuencia de reactividad cruzada con otras infecciones helmínticas. Las técnicas de inmunoensayo enzimático (ELISA) y doble difusión arco 5 (DD5) mostraron una sensibilidad de 73 y 57 % y una especificidad de 84-95% y 100%, respectivamente. La especificidad en la técnica de ELISA, fue mejorada sustancialmente al emplear como diluyente de los sueros una solución buffer conteniendo suero ovino normal y fosforilcolina. En líquido obtenido de hidátides de Echinococcus granulosus de origen ovino, se demostraron tres bandas de origen proteico de 64, 58 y 30 KDa de peso molecular, empleando SDS e inmunoblotting. Sueros de pacientes con estrongiloidiasis, oncocer-cosis, toxocariasis y cisticercosis reaccionaron con las bandas de 64 y 58 KDa en inmunoblotting. Sin embargo ninguno de los sueros reconoció la banda de 30 KD, la cual parece ser específica para este ensayo. La técnica de inmunobloting empleada para poner en evidencia la banda de 30 KDa con fines diagnósticos, exhibió una sensibilidad de 80% y una especificidad de 100%. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1994-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29155Revista do Instituto de Medicina Tropical de São Paulo; Vol. 36 No. 3 (1994); 205-210 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 36 Núm. 3 (1994); 205-210 Revista do Instituto de Medicina Tropical de São Paulo; v. 36 n. 3 (1994); 205-210 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29155/31012Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessPlanchart, SandraBotto, CarlosAlarcon de Noya, BelkisBonifacino, RosarioVivas, LiviaSpencer, LilianVivas, Sarai2012-07-02T01:37:37Zoai:revistas.usp.br:article/29155Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:54.448059Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Evaluation of the double diffusion, enzyme immunoassay and immunoblotting techniques, for the diagnosis of human hydatid disease in tropical areas Evaluación de las técnicas de Doble Difusión, Ensayo Inmunoenzimático e Inmunoblotting en el diagnóstico de la hidatidosis humana en áreas tropicales |
title |
Evaluation of the double diffusion, enzyme immunoassay and immunoblotting techniques, for the diagnosis of human hydatid disease in tropical areas |
spellingShingle |
Evaluation of the double diffusion, enzyme immunoassay and immunoblotting techniques, for the diagnosis of human hydatid disease in tropical areas Planchart, Sandra Hydatid Disease Cross Reactivity Double Diffusion ELISA Immunoblotting |
title_short |
Evaluation of the double diffusion, enzyme immunoassay and immunoblotting techniques, for the diagnosis of human hydatid disease in tropical areas |
title_full |
Evaluation of the double diffusion, enzyme immunoassay and immunoblotting techniques, for the diagnosis of human hydatid disease in tropical areas |
title_fullStr |
Evaluation of the double diffusion, enzyme immunoassay and immunoblotting techniques, for the diagnosis of human hydatid disease in tropical areas |
title_full_unstemmed |
Evaluation of the double diffusion, enzyme immunoassay and immunoblotting techniques, for the diagnosis of human hydatid disease in tropical areas |
title_sort |
Evaluation of the double diffusion, enzyme immunoassay and immunoblotting techniques, for the diagnosis of human hydatid disease in tropical areas |
author |
Planchart, Sandra |
author_facet |
Planchart, Sandra Botto, Carlos Alarcon de Noya, Belkis Bonifacino, Rosario Vivas, Livia Spencer, Lilian Vivas, Sarai |
author_role |
author |
author2 |
Botto, Carlos Alarcon de Noya, Belkis Bonifacino, Rosario Vivas, Livia Spencer, Lilian Vivas, Sarai |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Planchart, Sandra Botto, Carlos Alarcon de Noya, Belkis Bonifacino, Rosario Vivas, Livia Spencer, Lilian Vivas, Sarai |
dc.subject.por.fl_str_mv |
Hydatid Disease Cross Reactivity Double Diffusion ELISA Immunoblotting |
topic |
Hydatid Disease Cross Reactivity Double Diffusion ELISA Immunoblotting |
description |
Hydatid disease in tropical areas poses a serious diagnostic problem due to the high frequence of cross-reactivity with other endemic helminthic infections. The enzyme-linked-immunosorbent assay (ELISA) and the double diffusion arc 5 showed respectively a sensitivity of 73% and 57% and a specificity of 84-95% and 100%. However, the specificity of ELISA was greatly increased by using ovine serum and phosphorylcholine in the diluent buffer. The hydatic antigen obtained from ovine cyst fluid showed three main protein bands of 64,58 and 30 KDa using SDS PAGE and immunoblotting. Sera from patients with onchocerciasis, cysticercosis, toxocariasis and Strongyloides infection cross-reacted with the 64 and 58 KDa bands by immunoblotting. However, none of the analyzed sera recognized the 30 KDa band, that seems to be specific in this assay. The immunoblotting showed a sensitivity of 80% and a specificity of 100% when used to recognize the 30 KDa band. |
publishDate |
1994 |
dc.date.none.fl_str_mv |
1994-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29155 |
url |
https://www.revistas.usp.br/rimtsp/article/view/29155 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29155/31012 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 36 No. 3 (1994); 205-210 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 36 Núm. 3 (1994); 205-210 Revista do Instituto de Medicina Tropical de São Paulo; v. 36 n. 3 (1994); 205-210 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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1798951640972132352 |