In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/164174 |
Resumo: | Strongyloidiasis is a human parasitic disease caused by the helminth Strongyloides stercoralis whose treatment is particularly difficult in immunosuppressed patients due to their low responsiveness to conventional therapy. Carica papaya and its isolated compounds benzyl isothiocyanate, carpaine and carpasemine are promising compound for the treatment of Strongyloides infections due to their anthelmintic action. This study aims to examine the in vitro ovicidal and larvicidal activity of C. papaya seed hexane extract against Strongyloides venezuelensis, using egg hatching tests and larval motility tests as efficiency markers. The crude extract at the concentrations of 566 – 0.0566 mg/mL or the control with albendazole (0.025 mg/mL) and negative controls (water and PBS) were incubated with an equal volume of egg suspension (± 50 specimens) followed by counting of the specimens after 48 h. The same extract and dilutions were added to L3 larvae suspensions (±50 specimens) followed by analysis of larvae viability after 24, 48, and 72 h. The extract inhibited egg hatching with high efficiency at concentrations of 56.6 mg/mL (95.74%) and 5.66 mg/mL (92.16%). At the concentrations of 566 mg/mL (100%) and 56.66 mg/mL (97.32%), the extract inhibited larval motility as effectively as ivermectin (0.316 mg/mL; 100%), and more effectively than the other dilutions and the negative controls. The larvicidal effect depended on the extract concentration, but not on the treatment period. Therefore, C. papaya seed hexane extract has anthelmintic potential against S. venezuelensis and is a promising compound for the development of phytotherapies to treat strongyloidiasis. |
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In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensisCarica papayaSeed extractEgg hatching testLarval motility testAnthelmintic activityStrongyloidiasisStrongyloidesstercoralisStrongyloides venezuelensisStrongyloidiasis is a human parasitic disease caused by the helminth Strongyloides stercoralis whose treatment is particularly difficult in immunosuppressed patients due to their low responsiveness to conventional therapy. Carica papaya and its isolated compounds benzyl isothiocyanate, carpaine and carpasemine are promising compound for the treatment of Strongyloides infections due to their anthelmintic action. This study aims to examine the in vitro ovicidal and larvicidal activity of C. papaya seed hexane extract against Strongyloides venezuelensis, using egg hatching tests and larval motility tests as efficiency markers. The crude extract at the concentrations of 566 – 0.0566 mg/mL or the control with albendazole (0.025 mg/mL) and negative controls (water and PBS) were incubated with an equal volume of egg suspension (± 50 specimens) followed by counting of the specimens after 48 h. The same extract and dilutions were added to L3 larvae suspensions (±50 specimens) followed by analysis of larvae viability after 24, 48, and 72 h. The extract inhibited egg hatching with high efficiency at concentrations of 56.6 mg/mL (95.74%) and 5.66 mg/mL (92.16%). At the concentrations of 566 mg/mL (100%) and 56.66 mg/mL (97.32%), the extract inhibited larval motility as effectively as ivermectin (0.316 mg/mL; 100%), and more effectively than the other dilutions and the negative controls. The larvicidal effect depended on the extract concentration, but not on the treatment period. Therefore, C. papaya seed hexane extract has anthelmintic potential against S. venezuelensis and is a promising compound for the development of phytotherapies to treat strongyloidiasis.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2019-11-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/xmlhttps://www.revistas.usp.br/rimtsp/article/view/16417410.1590/S1678-9946201961059Revista do Instituto de Medicina Tropical de São Paulo; Vol. 61 (2019); e59Revista do Instituto de Medicina Tropical de São Paulo; Vol. 61 (2019); e59Revista do Instituto de Medicina Tropical de São Paulo; v. 61 (2019); e591678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/164174/157567https://www.revistas.usp.br/rimtsp/article/view/164174/157568Copyright (c) 2019 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessCabral, Eduardo Ramos MartinsMoraes, DayaneLevenhagen, Marcelo ArantesMatos, Ricardo Alexandre Figueiredo deCosta-Cruz, Julia MariaRodrigues, Rosângela Maria2019-11-18T17:29:33Zoai:revistas.usp.br:article/164174Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:50.584491Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis |
title |
In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis |
spellingShingle |
In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis Cabral, Eduardo Ramos Martins Carica papaya Seed extract Egg hatching test Larval motility test Anthelmintic activity Strongyloidiasis Strongyloidesstercoralis Strongyloides venezuelensis |
title_short |
In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis |
title_full |
In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis |
title_fullStr |
In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis |
title_full_unstemmed |
In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis |
title_sort |
In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis |
author |
Cabral, Eduardo Ramos Martins |
author_facet |
Cabral, Eduardo Ramos Martins Moraes, Dayane Levenhagen, Marcelo Arantes Matos, Ricardo Alexandre Figueiredo de Costa-Cruz, Julia Maria Rodrigues, Rosângela Maria |
author_role |
author |
author2 |
Moraes, Dayane Levenhagen, Marcelo Arantes Matos, Ricardo Alexandre Figueiredo de Costa-Cruz, Julia Maria Rodrigues, Rosângela Maria |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Cabral, Eduardo Ramos Martins Moraes, Dayane Levenhagen, Marcelo Arantes Matos, Ricardo Alexandre Figueiredo de Costa-Cruz, Julia Maria Rodrigues, Rosângela Maria |
dc.subject.por.fl_str_mv |
Carica papaya Seed extract Egg hatching test Larval motility test Anthelmintic activity Strongyloidiasis Strongyloidesstercoralis Strongyloides venezuelensis |
topic |
Carica papaya Seed extract Egg hatching test Larval motility test Anthelmintic activity Strongyloidiasis Strongyloidesstercoralis Strongyloides venezuelensis |
description |
Strongyloidiasis is a human parasitic disease caused by the helminth Strongyloides stercoralis whose treatment is particularly difficult in immunosuppressed patients due to their low responsiveness to conventional therapy. Carica papaya and its isolated compounds benzyl isothiocyanate, carpaine and carpasemine are promising compound for the treatment of Strongyloides infections due to their anthelmintic action. This study aims to examine the in vitro ovicidal and larvicidal activity of C. papaya seed hexane extract against Strongyloides venezuelensis, using egg hatching tests and larval motility tests as efficiency markers. The crude extract at the concentrations of 566 – 0.0566 mg/mL or the control with albendazole (0.025 mg/mL) and negative controls (water and PBS) were incubated with an equal volume of egg suspension (± 50 specimens) followed by counting of the specimens after 48 h. The same extract and dilutions were added to L3 larvae suspensions (±50 specimens) followed by analysis of larvae viability after 24, 48, and 72 h. The extract inhibited egg hatching with high efficiency at concentrations of 56.6 mg/mL (95.74%) and 5.66 mg/mL (92.16%). At the concentrations of 566 mg/mL (100%) and 56.66 mg/mL (97.32%), the extract inhibited larval motility as effectively as ivermectin (0.316 mg/mL; 100%), and more effectively than the other dilutions and the negative controls. The larvicidal effect depended on the extract concentration, but not on the treatment period. Therefore, C. papaya seed hexane extract has anthelmintic potential against S. venezuelensis and is a promising compound for the development of phytotherapies to treat strongyloidiasis. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-18 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/164174 10.1590/S1678-9946201961059 |
url |
https://www.revistas.usp.br/rimtsp/article/view/164174 |
identifier_str_mv |
10.1590/S1678-9946201961059 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/164174/157567 https://www.revistas.usp.br/rimtsp/article/view/164174/157568 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/xml |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 61 (2019); e59 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 61 (2019); e59 Revista do Instituto de Medicina Tropical de São Paulo; v. 61 (2019); e59 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
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